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Dive into the research topics where Isheng J. Tsai is active.

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Featured researches published by Isheng J. Tsai.


Nature | 2013

The genomes of four tapeworm species reveal adaptations to parasitism.

Isheng J. Tsai; Magdalena Zarowiecki; Nancy Holroyd; Alejandro Garciarrubio; Alejandro Sanchez-Flores; Karen Brooks; Alan Tracey; Raúl J. Bobes; Gladis Fragoso; Edda Sciutto; Martin Aslett; Helen Beasley; Hayley M. Bennett; Jianping Cai; Federico Camicia; Richard M. Clark; Marcela Cucher; Nishadi De Silva; Tim A. Day; Peter Deplazes; Karel Estrada; Cecilia Fernández; Peter W. H. Holland; Junling Hou; Songnian Hu; Thomas Huckvale; Stacy S. Hung; Laura Kamenetzky; Jacqueline A. Keane; Ferenc Kiss

Tapeworms (Cestoda) cause neglected diseases that can be fatal and are difficult to treat, owing to inefficient drugs. Here we present an analysis of tapeworm genome sequences using the human-infective species Echinococcus multilocularis, E. granulosus, Taenia solium and the laboratory model Hymenolepis microstoma as examples. The 115- to 141-megabase genomes offer insights into the evolution of parasitism. Synteny is maintained with distantly related blood flukes but we find extreme losses of genes and pathways that are ubiquitous in other animals, including 34 homeobox families and several determinants of stem cell fate. Tapeworms have specialized detoxification pathways, metabolism that is finely tuned to rely on nutrients scavenged from their hosts, and species-specific expansions of non-canonical heat shock proteins and families of known antigens. We identify new potential drug targets, including some on which existing pharmaceuticals may act. The genomes provide a rich resource to underpin the development of urgently needed treatments and control.


Genome Biology | 2010

Improving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps

Isheng J. Tsai; Thomas D. Otto; Matthew Berriman

Advances in sequencing technology allow genomes to be sequenced at vastly decreased costs. However, the assembled data frequently are highly fragmented with many gaps. We present a practical approach that uses Illumina sequences to improve draft genome assemblies by aligning sequences against contig ends and performing local assemblies to produce gap-spanning contigs. The continuity of a draft genome can thus be substantially improved, often without the need to generate new data.


PLOS Neglected Tropical Diseases | 2012

A systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni.

Anna V. Protasio; Isheng J. Tsai; A. K. Babbage; Sarah Nichol; Martin Hunt; Martin Aslett; Nishadi De Silva; Giles S. Velarde; Timothy J. C. Anderson; Richard Clark; Claire Davidson; Gary P. Dillon; Nancy Holroyd; Philip T. LoVerde; Christine Lloyd; Jacquelline McQuillan; Guilherme Oliveira; Thomas D. Otto; Sophia J. Parker-Manuel; Michael A. Quail; R. Alan Wilson; Adhemar Zerlotini; David W. Dunne; Matthew Berriman

Schistosomiasis is one of the most prevalent parasitic diseases, affecting millions of people in developing countries. Amongst the human-infective species, Schistosoma mansoni is also the most commonly used in the laboratory and here we present the systematic improvement of its draft genome. We used Sanger capillary and deep-coverage Illumina sequencing from clonal worms to upgrade the highly fragmented draft 380 Mb genome to one with only 885 scaffolds and more than 81% of the bases organised into chromosomes. We have also used transcriptome sequencing (RNA-seq) from four time points in the parasites life cycle to refine gene predictions and profile their expression. More than 45% of predicted genes have been extensively modified and the total number has been reduced from 11,807 to 10,852. Using the new version of the genome, we identified trans-splicing events occurring in at least 11% of genes and identified clear cases where it is used to resolve polycistronic transcripts. We have produced a high-resolution map of temporal changes in expression for 9,535 genes, covering an unprecedented dynamic range for this organism. All of these data have been consolidated into a searchable format within the GeneDB (www.genedb.org) and SchistoDB (www.schistodb.net) databases. With further transcriptional profiling and genome sequencing increasingly accessible, the upgraded genome will form a fundamental dataset to underpin further advances in schistosome research.


PLOS Pathogens | 2011

Genomic insights into the origin of parasitism in the emerging plant pathogen Bursaphelenchus xylophilus.

Taisei Kikuchi; James A. Cotton; Jonathan J. Dalzell; Koichi Hasegawa; Natsumi Kanzaki; Paul McVeigh; Takuma Takanashi; Isheng J. Tsai; Samuel A. Assefa; Peter J. A. Cock; Thomas D. Otto; Martin Hunt; Adam J. Reid; Alejandro Sanchez-Flores; Kazuko Tsuchihara; Toshiro Yokoi; Mattias C. Larsson; Johji Miwa; Aaron G. Maule; Norio Sahashi; John T. Jones; Matthew Berriman

Bursaphelenchus xylophilus is the nematode responsible for a devastating epidemic of pine wilt disease in Asia and Europe, and represents a recent, independent origin of plant parasitism in nematodes, ecologically and taxonomically distinct from other nematodes for which genomic data is available. As well as being an important pathogen, the B. xylophilus genome thus provides a unique opportunity to study the evolution and mechanism of plant parasitism. Here, we present a high-quality draft genome sequence from an inbred line of B. xylophilus, and use this to investigate the biological basis of its complex ecology which combines fungal feeding, plant parasitic and insect-associated stages. We focus particularly on putative parasitism genes as well as those linked to other key biological processes and demonstrate that B. xylophilus is well endowed with RNA interference effectors, peptidergic neurotransmitters (including the first description of ins genes in a parasite) stress response and developmental genes and has a contracted set of chemosensory receptors. B. xylophilus has the largest number of digestive proteases known for any nematode and displays expanded families of lysosome pathway genes, ABC transporters and cytochrome P450 pathway genes. This expansion in digestive and detoxification proteins may reflect the unusual diversity in foods it exploits and environments it encounters during its life cycle. In addition, B. xylophilus possesses a unique complement of plant cell wall modifying proteins acquired by horizontal gene transfer, underscoring the impact of this process on the evolution of plant parasitism by nematodes. Together with the lack of proteins homologous to effectors from other plant parasitic nematodes, this confirms the distinctive molecular basis of plant parasitism in the Bursaphelenchus lineage. The genome sequence of B. xylophilus adds to the diversity of genomic data for nematodes, and will be an important resource in understanding the biology of this unusual parasite.


Genome Biology | 2013

The genome and transcriptome of Haemonchus contortus, a key model parasite for drug and vaccine discovery

Roz Laing; Taisei Kikuchi; Axel Martinelli; Isheng J. Tsai; Robin N. Beech; Elizabeth Redman; Nancy Holroyd; David J. Bartley; Helen Beasley; Collette Britton; David M. Curran; Eileen Devaney; Aude Gilabert; Martin Hunt; Frank Jackson; Stephanie L Johnston; Ivan Kryukov; Keyu Li; Alison A. Morrison; Adam J. Reid; Neil Sargison; Gary Ian Saunders; James D. Wasmuth; Adrian J. Wolstenholme; Matthew Berriman; John S. Gilleard; James A. Cotton

BackgroundThe small ruminant parasite Haemonchus contortus is the most widely used parasitic nematode in drug discovery, vaccine development and anthelmintic resistance research. Its remarkable propensity to develop resistance threatens the viability of the sheep industry in many regions of the world and provides a cautionary example of the effect of mass drug administration to control parasitic nematodes. Its phylogenetic position makes it particularly well placed for comparison with the free-living nematode Caenorhabditis elegans and the most economically important parasites of livestock and humans.ResultsHere we report the detailed analysis of a draft genome assembly and extensive transcriptomic dataset for H. contortus. This represents the first genome to be published for a strongylid nematode and the most extensive transcriptomic dataset for any parasitic nematode reported to date. We show a general pattern of conservation of genome structure and gene content between H. contortus and C. elegans, but also a dramatic expansion of important parasite gene families. We identify genes involved in parasite-specific pathways such as blood feeding, neurological function, and drug metabolism. In particular, we describe complete gene repertoires for known drug target families, providing the most comprehensive understanding yet of the action of several important anthelmintics. Also, we identify a set of genes enriched in the parasitic stages of the lifecycle and the parasite gut that provide a rich source of vaccine and drug target candidates.ConclusionsThe H. contortus genome and transcriptome provide an essential platform for postgenomic research in this and other important strongylid parasites.


Nature Protocols | 2012

A post-assembly genome-improvement toolkit (PAGIT) to obtain annotated genomes from contigs

Martin T. Swain; Isheng J. Tsai; Samual A Assefa; Chris Newbold; Matthew Berriman; Thomas D. Otto

Genome projects now produce draft assemblies within weeks owing to advanced high-throughput sequencing technologies. For milestone projects such as Escherichia coli or Homo sapiens, teams of scientists were employed to manually curate and finish these genomes to a high standard. Nowadays, this is not feasible for most projects, and the quality of genomes is generally of a much lower standard. This protocol describes software (PAGIT) that is used to improve the quality of draft genomes. It offers flexible functionality to close gaps in scaffolds, correct base errors in the consensus sequence and exploit reference genomes (if available) in order to improve scaffolding and generating annotations. The protocol is most accessible for bacterial and small eukaryotic genomes (up to 300 Mb), such as pathogenic bacteria, malaria and parasitic worms. Applying PAGIT to an E. coli assembly takes ∼24 h: it doubles the average contig size and annotates over 4,300 gene models.


Genome Biology | 2014

The genome and life-stage specific transcriptomes of Globodera pallida elucidate key aspects of plant parasitism by a cyst nematode

James A. Cotton; Catherine J. Lilley; Laura M. Jones; Taisei Kikuchi; Adam J. Reid; Peter Thorpe; Isheng J. Tsai; Helen Beasley; Vivian C. Blok; Peter J. A. Cock; Sebastian Eves-van den Akker; Nancy Holroyd; Martin Hunt; Sophie Mantelin; Hardeep Naghra; Arnab Pain; Juan E. Palomares-Rius; Magdalena Zarowiecki; Matthew Berriman; John T. Jones; Peter E. Urwin

BackgroundGlobodera pallida is a devastating pathogen of potato crops, making it one of the most economically important plant parasitic nematodes. It is also an important model for the biology of cyst nematodes. Cyst nematodes and root-knot nematodes are the two most important plant parasitic nematode groups and together represent a global threat to food security.ResultsWe present the complete genome sequence of G. pallida, together with transcriptomic data from most of the nematode life cycle, particularly focusing on the life cycle stages involved in root invasion and establishment of the biotrophic feeding site. Despite the relatively close phylogenetic relationship with root-knot nematodes, we describe a very different gene family content between the two groups and in particular extensive differences in the repertoire of effectors, including an enormous expansion of the SPRY domain protein family in G. pallida, which includes the SPRYSEC family of effectors. This highlights the distinct biology of cyst nematodes compared to the root-knot nematodes that were, until now, the only sedentary plant parasitic nematodes for which genome information was available. We also present in-depth descriptions of the repertoires of other genes likely to be important in understanding the unique biology of cyst nematodes and of potential drug targets and other targets for their control.ConclusionsThe data and analyses we present will be central in exploiting post-genomic approaches in the development of much-needed novel strategies for the control of G. pallida and related pathogens.


Science | 2013

Genetic and Molecular Basis of Drug Resistance and Species-Specific Drug Action in Schistosome Parasites

Claudia L L Valentim; Donato Cioli; Frédéric D. Chevalier; Xiaohang Cao; Alexander B. Taylor; Stephen P. Holloway; Livia Pica-Mattoccia; Alessandra Guidi; Annalisa Basso; Isheng J. Tsai; Matthew Berriman; Claudia Carvalho-Queiroz; Marcio Almeida; Hector R. Aguilar; Doug E. Frantz; P. John Hart; Philip T. LoVerde; Timothy J. C. Anderson

Blood Fluke Resistance The larval stages of the blood fluke Schistosoma mansoni are disseminated via a replicative cycle in freshwater snails. When people come into contact with contaminated water, the larvae attach to and penetrate the skin. The resulting disease, bilharzia or schistosomiasis, afflicts approximately 67 million people in Africa and South America. Unfortunately, the parasite is showing resistance to one of the available therapeutic drugs, oxamniquine, which means that schistosome control relies on a single drug, praziquantel. Valentim et al. (p. 1385, published online 21 November) analyzed the genetic and molecular basis of resistance to oxamniquine through a combination of genetic linkage mapping, genome sequencing, functional genomics analysis, and x-ray crystallography. Mutations in a distinctive sulfotransferase are responsible for oxamniquine resistance in a human blood fluke. Oxamniquine resistance evolved in the human blood fluke (Schistosoma mansoni) in Brazil in the 1970s. We crossed parental parasites differing ~500-fold in drug response, determined drug sensitivity and marker segregation in clonally derived second-generation progeny, and identified a single quantitative trait locus (logarithm of odds = 31) on chromosome 6. A sulfotransferase was identified as the causative gene by using RNA interference knockdown and biochemical complementation assays, and we subsequently demonstrated independent origins of loss-of-function mutations in field-derived and laboratory-selected resistant parasites. These results demonstrate the utility of linkage mapping in a human helminth parasite, while crystallographic analyses of protein-drug interactions illuminate the mode of drug action and provide a framework for rational design of oxamniquine derivatives that kill both S. mansoni and S. haematobium, the two species responsible for >99% of schistosomiasis cases worldwide.


Nature Genetics | 2014

Whipworm genome and dual-species transcriptome analyses provide molecular insights into an intimate host-parasite interaction

Bernardo J. Foth; Isheng J. Tsai; Adam J. Reid; Allison J. Bancroft; Sarah Nichol; Alan Tracey; Nancy Holroyd; James A. Cotton; Eleanor Stanley; Magdalena Zarowiecki; Jimmy Z. Liu; Thomas Huckvale; Philip J Cooper; Richard K. Grencis; Matthew Berriman

Whipworms are common soil-transmitted helminths that cause debilitating chronic infections in man. These nematodes are only distantly related to Caenorhabditis elegans and have evolved to occupy an unusual niche, tunneling through epithelial cells of the large intestine. We report here the whole-genome sequences of the human-infective Trichuris trichiura and the mouse laboratory model Trichuris muris. On the basis of whole-transcriptome analyses, we identify many genes that are expressed in a sex- or life stage–specific manner and characterize the transcriptional landscape of a morphological region with unique biological adaptations, namely, bacillary band and stichosome, found only in whipworms and related parasites. Using RNA sequencing data from whipworm-infected mice, we describe the regulated T helper 1 (TH1)-like immune response of the chronically infected cecum in unprecedented detail. In silico screening identified numerous new potential drug targets against trichuriasis. Together, these genomes and associated functional data elucidate key aspects of the molecular host-parasite interactions that define chronic whipworm infection.


Nature Genetics | 2016

The genomic basis of parasitism in the Strongyloides clade of nematodes

Vicky L. Hunt; Isheng J. Tsai; Avril Coghlan; Adam J. Reid; Nancy Holroyd; Bernardo J. Foth; Alan Tracey; James A. Cotton; Eleanor Stanley; Helen Beasley; Hayley M. Bennett; Karen Brooks; Bhavana Harsha; Rei Kajitani; Arpita Kulkarni; Dorothee Harbecke; Eiji Nagayasu; Sarah Nichol; Yoshitoshi Ogura; Michael A. Quail; Nadine P. Randle; Dong Xia; Norbert W. Brattig; Hanns Soblik; Diogo M Ribeiro; Alejandro Sanchez-Flores; Tetsuya Hayashi; Takehiko Itoh; Dee R. Denver; Warwick N. Grant

Soil-transmitted nematodes, including the Strongyloides genus, cause one of the most prevalent neglected tropical diseases. Here we compare the genomes of four Strongyloides species, including the human pathogen Strongyloides stercoralis, and their close relatives that are facultatively parasitic (Parastrongyloides trichosuri) and free-living (Rhabditophanes sp. KR3021). A significant paralogous expansion of key gene families—families encoding astacin-like and SCP/TAPS proteins—is associated with the evolution of parasitism in this clade. Exploiting the unique Strongyloides life cycle, we compare the transcriptomes of the parasitic and free-living stages and find that these same gene families are upregulated in the parasitic stages, underscoring their role in nematode parasitism.

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Matthew Berriman

Wellcome Trust Sanger Institute

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Nancy Holroyd

Wellcome Trust Sanger Institute

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James A. Cotton

Wellcome Trust Sanger Institute

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Adam J. Reid

Wellcome Trust Sanger Institute

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Alan Tracey

Wellcome Trust Sanger Institute

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Martin Hunt

Wellcome Trust Sanger Institute

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Eleanor Stanley

Wellcome Trust Sanger Institute

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