J. C. Woodworth

Interactive effects between paylean™ (Ractopamine HCl) and dietary lysine on finishing pig growth performance, carcass characteristics and tissue accretion

Summary A total of 432 pigs were used to evaluate the effects of Paylean and dietary lysine on finishing pig growth performance, carcass characteristics and tissue accretion. The 12 dietary treatments included Paylean (0, 4.5, and 9.0 g/ton) and 4 levels of lysine. For pigs fed no Paylean, lysine levels were 0.60, 0.80, 1.00, and 1.20%. For pigs fed 4.5 or 9.0 g/ton of Paylean, lysine levels were 0.80, 1.00, 1.20, and 1.40%. The results indicate that pigs fed Paylean need at least 1.0% dietary lysine to optimize growth, carcass parameters, and tissue accretion.

Effects of l-carnitine supplementation on quality characteristics of fresh pork bellies from pigs fed 3 levels of corn oil1

Crossbred pigs (n = 216) were used to test the effect of supplemental L-carnitine (CARN) on the fatty acid composition and quality characteristics of fresh pork bellies from pigs fed diets formulated with different inclusion levels of corn oil. Pigs were blocked by BW (43.6 ± 1.0 kg) and allotted randomly to pens of 6 pigs within blocks. Then, within blocks, pens were assigned randomly to 1 of 6 dietary treatments in a 2 × 3 factorial arrangement, with either 0 or 100 mg/kg of supplemental CARN and 3 dietary inclusion levels (0, 2, or 4%) of corn oil (CO). When the lightest block weighed 125.0 kg, all pigs were slaughtered, and left-side bellies were captured during carcass fabrication for quality data collection. Fresh pork bellies were evaluated for length, width, thickness, and firmness (bar-suspension and Instron-compression methods) before a 2.5-cm-wide strip of belly was removed and subsequently dissected into subcutaneous fat, primary lean (latissimus dorsi), secondary lean (cutaneous trunci), and intermuscular fat for fatty acid composition determination. Although belly length, width, and thickness of fresh pork bellies were not affected by CARN (P ≥ 0.128) or CO (P ≥ 0.073), belly firmness decreased linearly (P < 0.001) with increasing dietary CO, but there was no (P ≥ 0.137) effect of CARN on any belly firmness measure. Dietary CARN increased (P < 0.05) the proportion of total SFA in the intermuscular fat layer, increased (P < 0.05) the proportion of total MUFA in the primary and secondary lean layers, and decreased (P < 0.05) the proportion of total PUFA in the intermuscular fat and secondary lean layers of pork bellies. Moreover, the SFA and MUFA compositions decreased linearly (P < 0.001) with increasing dietary CO, and the rate of the decrease in SFA composition was greater (P < 0.001) in the fat layers than the lean layers. Conversely, the PUFA content increased linearly (P < 0.001) with increasing dietary CO, and the rate of the increase in PUFA was greater (P < 0.001) in the fat than the lean layers, and greater (P = 0.022) in the primary than secondary lean layer. Results from this study would indicate that differences in the amount and rate of fatty acid deposition associated with feeding increased amounts of CO, along with moisture differences among the belly layers, combine to negatively affect fresh pork belly firmness.

Maternal Dietary l-Carnitine Supplementation Influences Fetal Carnitine Status and Stimulates Carnitine Palmitoyltransferase and Pyruvate Dehydrogenase Complex Activities in Swine

Effects of increasing maternal L-carnitine on carnitine status and energy metabolism in the fetus were evaluated by feeding pregnant swine a corn-soybean-based diet containing either 0 or 50 mg/kg added L-carnitine (n = 10/treatment) during the first 70 d of gestation. Carnitine, carnitine palmitoyltransferase (CPT), and pyruvate dehydrogenase complex (PDHC) activities were analyzed in tissues collected from fetuses on d 55 and 70. Maternal L-carnitine supplementation increased both fetal free and long-chain carnitine concentrations by 45% in liver and free carnitine by 31% in heart tissues but did not affect kidney tissue. Elevations in free and acylcarnitines increased with gestational age from 55 to 70 d in liver but not in heart and kidney. The increased carnitine concentrations resulted in a 45% increase in PDHC activity in heart and liver on d 70 of gestation but did not affect kidney and liver on d 55 of gestation. The increases in carnitine concentrations were accompanied by a 70% increase in hepatic CPT activity in 70-d-old fetuses, but activities in heart and kidney were unaffected. The Michaelis constant (K(m)) of CPT for carnitine in fetal tissues was not influenced by carnitine supplementation (P > 0.1). Notably, the concentrations of carnitine measured on d 70 were only 25-40% of the K(m) values in liver, 60-70% in heart, and 30-40% in kidney (P < 0.001). We conclude that carnitine ingestion during pregnancy increases fetal carnitine concentrations and stimulates heart PDHC and liver CPT activity without altering carnitine K(m).

Effects of feeding modified tall oil and supplemental potassium and magnesium on growth performance, carcass characteristics, and meat quality of growing-finishing pigs

Eighty crossbred gilts (initially 45.9 kg) were allotted randomly to one of four dietary treatments by weight and ancestry. The trial was arranged as a 2 × 2 factorial with two levels of modified tall oil (MTO) (0 or 0.50%) and added K2SO4-2MgSO4 (0 or 2%), equating to daily K and Mg intakes of 10.84 and 7.75 g, respectively. The corn-soybean meal diets were fed in two phases [45.9 to 76.2 and 76.2 to 118.1 kg body weight (BW)], and supplemental K/Mg was added in place of corn for the final 7 d preslaughter (starting at 114.1 kg BW). Dietary treatment did not affect (P > 0.10) average daily gain (ADG), average daily feed intake (ADFI), or gain to feed ratio (G/F). Feeding MTO decreased average backfat (P = 0.05) and increased intramuscular marbling (P = 0.04). Modified tall oil increased (P = 0.02) percentage lean, and K/Mg supplementation lowered (P = 0.04) longissimus muscle glycogen content. Dietary treatment did not affect (P > 0.10) other carcass characteristics or measures of meat quality. Feeding M...

Effects of dietary L-carnitine and ractopamine HCl on the metabolic response to handling in finishing pigs,

Two experiments (384 pigs; C22 × L326; PIC) were conducted to determine the interactive effect of dietary L-carnitine and ractopamine HCl (RAC) on the metabolic response of pigs to handling. Experiments were arranged as split-split plots with handling as the main plot and diets as subplots (4 pens per treatment). Dietary L-carnitine (0 or 50 mg/kg) was fed from 36.0 kg to the end of the experiments (118 kg), and RAC (0 or 20 mg/kg) was fed the last 4 wk of each experiment. At the end of each experiment, 4 pigs per pen were assigned to 1 of 2 handling treatments. Gently handled pigs were moved at a moderate walking pace 3 times through a 50-m course and up and down a 15° loading ramp. Aggressively handled pigs were moved as fast as possible 3 times through the same course, but up and down a 30° ramp, and shocked 3 times with an electrical prod. Blood was collected immediately before and after handling in Exp. 1 and immediately after and 1 h after handling in Exp. 2. Feeding RAC increased (P < 0.01) ADG and G:F, but there was no effect (P > 0.10) of L-carnitine on growth performance. In Exp. 1 and 2, aggressive handling increased (P < 0.01) blood lactate dehydrogenase (LDH), lactate, cortisol, and rectal temperature and decreased blood pH. In Exp. 1, there was a RAC × handling interaction (P < 0.06) for the difference in pre- and posthandling blood pH and rectal temperature. Aggressively handled pigs fed RAC had decreased blood pH and increased rectal temperature compared with gently handled pigs, demonstrating the validity of the handling model. Pigs fed RAC had increased (P < 0.01) LDH compared with pigs not fed RAC. Pigs fed L-carnitine had increased (P < 0.03) lactate compared with pigs not fed L-carnitine. In Exp. 2, pigs fed RAC had lower (P < 0.02) blood pH immediately after handling, but pH returned to control levels by 1 h posthandling. Lactate, LDH, cortisol, and rectal temperature changes from immediately posthandling to 1 h posthandling were not different (P > 0.10) between pigs fed L-carnitine and those fed RAC, indicating that L-carnitine did not decrease recovery time of pigs subjected to aggressive handling. These results suggest that pigs fed 20 mg/kg of RAC are more susceptible to stress when handled aggressively compared with pigs not fed RAC. Dietary L-carnitine fed in combination with RAC did not alleviate the effects of stress. This research emphasizes the importance of using proper animal handling techniques when marketing finishing pigs fed RAC.

Effects of feeding L-carnitine to gilts through day 70 of gestation on litter traits and the expression of insulin-like growth factor system components and L-carnitine concentration in foetal tissues.

We investigated the influence of supplemental L-carnitine on foetal blood metabolites, litter characteristics, L-carnitine concentration in skeletal muscle and insulin-like growth factor (IGF) axis components in foetal hepatic and skeletal muscle tissues at day 40, 55 and 70 of gestating gilts. A total of 59 gilts (body weight = 137.7 kg) received a constant feed allowance of 1.75 kg/day and a top-dress containing either 0 or 50 ppm of L-carnitine starting on the first day of breeding through the allotted gestation length. Foetuses from the gilts fed diets with L-carnitine tended to be heavier (p = 0.06) and the circulating IGF-II tended to be lower (p = 0.09) at day 70, compared with the foetuses from the control gilts. Insulin-like growth factor-I messenger RNA (mRNA) was lower (p = 0.05) in hepatic tissue in the foetuses collected from gilts fed L-carnitine. Free and total carnitine concentration increased (p < 0.05) in the skeletal muscle from the foetuses collected from gilts fed supplemental L-carnitine. This study showed that L-carnitine had beneficial effects on the average foetal weight at day 70 of gestation, associated with changes in the foetal IGF system.

Effects of dietary additions of modified tall oil, chromium nicotinate, and L-carnitine on growth performance, carcass characteristics, and bacon characteristics of growing-finishing pigs

Eighty gilts were supplemented with modified tall oil (MTO), chromium nicotinate (CrNic), and L-carnitine to determine effects on growth and meat quality characteristics. Pigs were assigned to one of eight treatments in a 2 × 2 × 2 factorial with main effects of MTO (0 or 0.5%), CrNic (0 or 50 μg kg-1), and L-carnitine (0 or 50 mg kg-1). Pigs fed MTO had increased (P = 0.03) average daily gain and pigs fed CrNic had improved (P = 0.02) gain:feed. Bellies from pigs supplemented with MTO with no CrNic were firmer (P 0.05) were detected for longissimus muscle (LM) visual or objective color values. Furthermore, no differences (P > 0.05) were detected for LM Warner-Bratzler shear force or sensory traits. Bacon from pigs fed MTO had firmer (P < 0.05) slices than bacon from pigs fed no MTO.These data suggest improvements in growth performance from addition of 0.50% MTO and(or) 50 μg kg-1 CrNic to diets of finishing gilts. Supplementi...

Interactive effects of dietary ractopamine HCl and L-carnitine on finishing pigs: I. Growth performance.

A total of 2,152 pigs (C22 × 336 PIC) were used in 4 experiments to determine the interactive effects of dietary l-carnitine and ractopamine HCl (RAC) on finishing pig growth performance. All trials were arranged as factorial arrangements with main effects of l-carnitine (0, 25, or 50 mg/kg in Exp. 1 and 2 and 0 or 50 mg/kg in Exp. 3 and 4) and RAC (0, 5, or 10 mg/kg in Exp. 1 and 0 or 10 mg/kg in Exp. 2, 3, and 4). Dietary carnitine was fed from 38 to 109 kg (Exp. 1 and 3) or for the last 4 or 3 wk before slaughter (118 kg; Exp. 2 and 4, respectively). Ractopamine HCl was fed for 4 wk (Exp. 1, 2, and 3) or 3 wk (Exp. 4) before slaughter. Experiments 1 and 2 were conducted in university research facilities, and Exp. 3 and 4 were conducted in a commercial research facility. All diets were formulated to contain 1.00% total Lys during the last phase of each experiment. In all experiments, pigs fed RAC had increased (P < 0.05) ADG and G:F compared with pigs fed no RAC. Feeding l-carnitine before the RAC feeding period did not affect pig growth performance. In Exp. 1 and 2, l-carnitine did not affect ADG during the last 4 wk; however, in Exp. 2, G:F tended (quadratic; P = 0.07) to improve with increasing l-carnitine. In Exp. 3, l-carnitine × RAC interactions were observed (P < 0.04) for ADG and G:F. Both added l-carnitine and RAC improved performance, but the response was not additive. In Exp. 4, pigs fed l-carnitine had increased (P < 0.04) ADG (0.88 vs. 0.84 kg) and G:F (0.36 vs. 0.35) compared with pigs fed no l-carnitine, and the response was additive to that of RAC. Analysis of treatments common to all experiments showed that pigs fed RAC had increased (P < 0.01) ADG (1.03 vs. 0.93 kg) and G:F (0.40 vs. 0.35) compared with pigs fed no RAC. Pigs fed l-carnitine tended to have increased (P = 0.07) ADG (1.00 vs. 0.96 kg) and improved (P < 0.01) G:F (0.38 vs. 0.37) compared with pigs not fed l-carnitine. These results confirm that RAC improves growth performance of finishing pigs. Added l-carnitine improved growth performance of finishing pigs, and the greatest response was observed in Exp. 3 and 4, which were conducted in commercial research environments. These experiments imply that adding l-carnitine to a finishing diet does not enhance the growth effects of RAC and that effects of RAC and l-carnitine on ADG and G:F are independent.

Interactive effects of dietary ractopamine HCl and L-carnitine on finishing pigs: II. Carcass characteristics and meat quality

Three experiments using 1,356 pigs (C22 × 336 PIC) were conducted to determine the interactive effects of dietary L-carnitine and ractopamine hydrochloride (RAC) on carcass characteristics and meat quality of finishing pigs. Experiments were arranged as factorials with main effects of L-carnitine and RAC; L-carnitine levels were 0, 25, or 50 mg/kg in Exp. 1 and 2 and 0 or 50 mg/kg in Exp. 3, and RAC levels of 0, 5, or 10 mg/kg in Exp. 1 and 0 or 10 mg/kg in Exp. 2 and 3. Dietary L-carnitine was fed from 38 kg to slaughter (109 and 118 kg in Exp. 1 and 3, respectively) or for 4 wk before slaughter (109 kg in Exp. 2). Ractopamine HCl was fed for 4 wk in all experiments. Exp. 1 and 2 were conducted at university research facilities (2 pigs per pen), and Exp. 3 was conducted in a commercial research barn (23 pigs per pen). In Exp. 1, an L-carnitine × RAC interaction (P < 0.02) was observed for LM visual color, L*, and a*/b*. In pigs fed RAC, increasing L-carnitine decreased L* and increased visual color scores and a*/b* compared with pigs not fed RAC. Ultimate pH tended to increase (linear, P < 0.07) with increasing L-carnitine. Drip loss decreased (linear, P < 0.04) in pigs fed increasing L-carnitine. In Exp. 2, firmness scores decreased in pigs fed increasing L-carnitine when not fed RAC, but firmness scores increased and drip losses decreased with increasing L-carnitine when RAC was added to the diet (L-carnitine × RAC interaction, P < 0.04). Percentage lean was greater (P < 0.01) for pigs fed RAC in Exp. 2. In Exp. 3, fat thickness decreased and lean percentage increased in pigs fed L-carnitine or RAC, but the responses were not additive (L-carnitine × RAC interaction, P < 0.03). Furthermore, pigs fed L-carnitine tended (P < 0.06) to have decreased LM drip loss percentage whereas pigs fed RAC had decreased (P < 0.05) 10th rib and average backfat and decreased drip loss than pigs fed diets without RAC. These results suggest that dietary RAC increased carcass leanness and supplemental L-carnitine reduced LM drip loss when fed in combination with RAC.

Re-evaluating Floor Space Allowance and Removal Strategy Effects on the Growth of Heavyweight Finishing Pigs

This study was performed to evaluate the impact of initial floor space allowance and various topping strategies (removal of the heaviest pigs in a pen prior to marketing the finishing group) on the growth performance of heavyweight finishing pigs. A total of 1,092 pigs (initially 80.1 lb) were allotted to one of 4 experimental treatments with 14 pens per treatment. The first treatment stocked pigs at 9.7 ft2 (15 pigs/pen) throughout the study. The other three treatments initially stocked pigs at 6.9 ft2. The second treatment (2:2:2) topped the two heaviest pigs on d 64 (203 lb), d 76 (227 lb), and d 95 (264 lb), which coincided with the time floor space allowance became limiting, as predicted by Gonyou et al. (2006). The third treatment (2:4) topped the 2 heaviest pigs and the 4 heaviest pigs at an average BW of 240 (d 76) and 280 lb (d 105), respectively. The fourth treatment (6) topped the 6 heaviest pigs at an average BW of 280 lb (d 105). All pigs remaining in pens after topping events were marketed on d 117 of the study. Overall (d 0 to 117), pigs in pens stocked at 9.7 ft2 had increased (P < 0.05) ADG compared to pigs in pens on either the 2:4 or 6 topping strategies, but ADG was not different from pigs in pens on the 2:2:2 topping strategy. This suggests that prediction equations developed by Gonyou et al. (2006) for ADG are useful for predicting the effects of floor space on heavyweight pig ADG. Pigs in pens stocked at 9.7 ft2 had increased (P < 0.05) ADFI compared to pigs in pens initially stocked at 6.9 ft2 regardless of topping strategy. Total weight gain per pen was greater (P < 0.05) for pens initially stocked at 6.9 ft2 compared to pens stocked at 9.7 ft2; however, total weight gain per pig was greater for pigs in pens stocked at 9.7 ft2 compared to pigs in pens initially stocked at 6.9 ft2. Pigs in pens on the 2:2:2 topping strategy had less weight gain (P < 0.05) than pigs in pens on the 6 topping strategy. Feed usage per pen was decreased for pens stocked at 9.7 ft2 compared to those initially stocked at 6.9 ft2; however, per pig feed usage was increased (P < 0.05) for pigs in pens stocked at 9.7 ft2 compared to pigs in pens initially stocked at 6.9 ft2. Pens on the 2:2:2 topping strategy had less (P < 0.05) feed usage, either on a pen or pig basis, than those on the 2:4 or the 6 topping strategy. Interestingly, there was a tendency (P < 0.10) for pigs in pens on the 2:4 topping strategy to have less feed usage than pigs in pens on the 6 topping strategy. Income over feed and facility cost (IOFFC) was decreased (P < 0.05), either on a pen or pig basis, for pens stocked at 9.7 ft2. Pigs in pens on the 2:2:2 topping strategy had numerically less IOFFC when revenue was high and feed cost was low compared to pigs in pens on the 2:4 or 6 topping strategy. In conclusion, increasing the floor space allowance or the time points at which pigs are removed from the pen improved the performance of pigs remaining in the pen; however, IOFFC may be reduced due to fewer pigs marketed from each pen (in the case of lower stocking density) or from reducing total weight produced (in pens where pigs are topped earlier at lighter weights).