James S. Bennett

Spatially-resolved rotational microrheology with an optically-trapped sphere

We have developed a microrheometer, based on optical tweezers, in which hydrodynamic coupling between the probe and fluid boundaries is dramatically reduced relative to existing microrheometers. Rotational Brownian motion of a birefringent microsphere within an angular optical trap is observed by measuring the polarisation shifts of transmitted light. Data gathered in this manner, in the strongly viscoelastic fluid Celluvisc, quantitatively agree with the results of conventional (bulk) rheometry. Our technique will significantly reduce the smallest sample volumes which may be reliably probed, further extending the study of rare, difficult to obtain or highly nonhomogeneous fluids.

A quantum optomechanical interface beyond the resolved sideband limit

Mechanical oscillators which respond to radiation pressure are a promising means of transferring quantum information between light and matter. Optical--mechanical state swaps are a key operation in this setting. Existing proposals for optomechanical state swap interfaces are only effective in the resolved sideband limit. Here, we show that it is possible to fully and deterministically exchange mechanical and optical states outside of this limit, in the common case that the cavity linewidth is larger than the mechanical resonance frequency. This high-bandwidth interface opens up a significantly larger region of optomechanical parameter space, allowing generation of non-classical motional states of high-quality, low-frequency mechanical oscillators.

Factors affecting the f× Q product of 3C-SiC microstrings: what is the upper limit for sensitivity?

The fn × Q (Hz) is a crucial sensitivity parameter for micro-electro-mechanical sensing. We have recently shown a fn × Q product of ∼1012 Hz for microstrings made of cubic silicon carbide on silicon, establishing a new state-of-the-art and opening new frontiers for mass sensing applications. In this work, we analyse the main parameters influencing the frequency and quality factor of silicon carbide microstrings (material properties, microstring geometry, clamping condition, and environmental pressure) and investigate the potential for approaching the theoretical upper limit. We indicate that our previous result is only about a factor 2 lower than the thermoelastic dissipation limit. For fully reaching this upper limit, a substantial reduction of the defects in the silicon carbide thin film would be required, while maintaining a high residual tensile stress in the perfect-clamped strings.

Coherent control and feedback cooling in a remotely coupled hybrid atom–optomechanical system

Cooling to the motional ground state is an important first step in the preparation of nonclassical states of mesoscopic mechanical oscillators. Light-mediated coupling to a remote atomic ensemble has been proposed as a method to reach the ground state for low frequency oscillators. The ground state can also be reached using optical measurement followed by feedback control. Here we investigate the possibility of enhanced cooling by combining these two approaches. The combination, in general, outperforms either individual technique, though atomic ensemble-based cooling and feedback cooling each individually dominate over large regions of parameter space.

Phenotypic and molecular dissection of Metaplastic Breast Cancer and the prognostic implications: Prognostic features of Metaplastic breast cancer.

Metaplastic breast carcinoma (MBC) is relatively rare but accounts for a significant proportion of global breast cancer mortality. This group is extremely heterogeneous and by definition exhibits metaplastic change to squamous and/or mesenchymal elements, including spindle, squamous, chondroid, osseous, and rhabdomyoid features. Clinically, patients are more likely to present with large primary tumours (higher stage), distant metastases, and overall, have shorter 5‐year survival compared to invasive carcinomas of no special type. The current World Health Organisation (WHO) diagnostic classification for this cancer type is based purely on morphology – the biological basis and clinical relevance of its seven sub‐categories are currently unclear. By establishing the Asia‐Pacific MBC (AP‐MBC) Consortium, we amassed a large series of MBCs (n = 347) and analysed the mutation profile of a subset, expression of 14 breast cancer biomarkers, and clinicopathological correlates, contextualising our findings within the WHO guidelines. The most significant indicators of poor prognosis were large tumour size (T3; p = 0.004), loss of cytokeratin expression (lack of staining with pan‐cytokeratin AE1/3 antibody; p = 0.007), EGFR overexpression (p = 0.01), and for ‘mixed’ MBC, the presence of more than three distinct morphological entities (p = 0.007). Conversely, fewer morphological components and EGFR negativity were favourable indicators. Exome sequencing of 30 cases confirmed enrichment of TP53 and PTEN mutations, and intriguingly, concurrent mutations of TP53, PTEN, and PIK3CA. Mutations in neurofibromatosis‐1 (NF1) were also overrepresented [16.7% MBCs compared to ∼5% of breast cancers overall; enrichment p = 0.028; mutation significance p = 0.006 (OncodriveFM)], consistent with published case reports implicating germline NF1 mutations in MBC risk. Taken together, we propose a practically minor but clinically significant modification to the guidelines: all WHO_1 mixed‐type tumours should have the number of morphologies present recorded, as a mechanism for refining prognosis, and that EGFR and pan‐cytokeratin expression are important prognostic markers. Copyright

Rapid mechanical squeezing with pulsed optomechanics

Macroscopic mechanical oscillators can be prepared in quantum states and coherently manipulated using the optomechanical interaction. This has recently been used to prepare squeezed mechanical states. However, the scheme used in these experiments relies on slow, dissipative evolution that destroys the systems memory of its initial state. In this paper we propose a protocol based on a sequence of four pulsed optomechanical interactions. In addition to being coherent, our scheme executes in a time much shorter than a mechanical period. We analyse applications in impulsive force sensing and preservation of Schrodinger cat states, which are useful in continuous-variable quantum information protocols.

‘Wiggler-Waggler’ — Optical measurements of complex viscoelastic moduli

We demonstrate the simultaneous optical measurement of the viscoelastic modulus at many frequencies by active rotational ‘Fourier’ microrheology. This was achieved by employing a birefringent vaterite microsphere probe in a dual-beam optical tweezers apparatus.

'Wiggler-Waggler': towards optical measurements of the complex shear modulus

Microrheology, the study of flow at the microscopic scale, has benefited immensely from a variety of optical micromanipulation techniques developed over the past two decades. However, very few present procedures allow the rapid measurement of the viscoelastic properties of fluid samples with volumes on the order of tens of picolitres over a wide frequency range. We detail preliminary construction and analysis of an active rotational microrheological method which promises to achieve this. Rotational microrheology was performed by optically trapping a birefringent probe particle in a linearly polarised dual-beam trap and rapidly rotating the polarisation direction through a xed angle. This provides measurements of the low-frequency fluid response, whilst passive monitoring of thermal motion is used to determine high-frequency components. Our method is less sensitive to boundary effects and probe particle asphericity than analogous translational microrheological techniques, so will be ideal for microfluidic applications and analysis of fluids which are generally available in volumes which preclude the use of existing experimental techniques.