John M. Macauley

DISSOLVED OXYGEN CONDITIONS IN NORTHERN GULF OF MEXICO ESTUARIES

Because deficient dissolved oxygen (DO) levels may have severe detrimental effects on estuarine and marine life, DO has been widely used as an indicator of ecological conditions by environmental monitoring programs. The U.S. EPAs Environmental Monitoring and Assessment Program for Estuaries (EMAP-E) monitored DO conditions in the estuaries of the Gulf of Mexico from 1991 to 1994. DO was measured in two ways: 1) instantaneous profiles from the surface to the bottom were taken during the day, and 2) continuous measurements were taken near the bottom at 15 min intervals for at least 12 h. This information was summarized to assess the spatial distribution and severity of DO conditions in these estuaries. Depending on the criteria used to define hypoxia (DO concentrations usually <2 mg L-1 or <5 mg L-1) and the method by which DO is measured, we estimate that between 5.2 and 29.3% of the total estuarine area in the Louisianian Province was affected by low DO conditions.

Effects of light reduction on growth of the submerged macrophyte Vallisneria americana and the community of root-associated heterotrophic bacteria

Abstract A shading experiment was conducted over a growing season to measure the effects of light reduction on Vallisneria americana in Perdido Bay on the Florida–Alabama border and to determine the response of heterotrophic bacteria in the rhizosphere. Plants subjected to 92% light reduction showed the most pronounced effects in chlorophyll a concentration, above- and below-ground biomass, and leaf dimensions. These results further suggested that the V. americana life cycle, as exhibited in temperate waters, was impaired. Heterotrophic bacteria were enumerated and identified (i) from the roots and sediments of fully illuminated plants and from unvegetated sediments at three intervals and (ii) from the roots of plants that have been subjected to 92% light reduction for 3 months. Up to two orders of magnitude greater numbers of bacteria were enumerated from root samples than sediment samples on a dry weight basis. Bacteria enumerated from the roots of plants subjected to light reduction (1.3±1.1×10 8 CFU g −1 ) were significantly higher than numbers of bacteria enumerated from the roots of fully illuminated plants (4.8±1.8×10 7 g −1 in the summer) or sediment samples (1.4±0.03×10 6 g −1 ). This suggests the roots of seagrasses stressed by light reduction provided more nutrients for bacterial growth. Higher percentages of Gram-negative bacteria were isolated from roots (up to 85% in the fall) than sediments (0–15%). Examination of isolates for traits characteristic of rhizosphere bacteria (siderophore production, formation of the phytohormone indole-3-acetic acid, and antifungal activity) did not show a clear distinction between root-associated and sediment isolates. Taxonomic identifications of root-associated bacteria based on MIDI analysis of fatty acid methyl esters were consistent with bacteria known to be associated with other plants or found at oxic–anoxic interfaces. In addition, the bacterial identifications showed most species were associated with only roots or only sediments. These results support studies suggesting seagrass rhizospheres harbor distinct bacterial communities.

Seasonal changes in the standing crop and chlorophyll content of Thalassia testudinum Banks ex König and its epiphytes in the northern Gulf of Mexico

Abstract The seasonal cycles for standing crop and chlorophyll content of Thalassia testudinum Banks ex Konig and its epiphytes are described from monitoring data collected at a study site in Santa Rosa Sound, northwestern Florida, from December 1983 through March 1987. Water temperature correlated more highly with standing crop and chlorophyll measurements than did salinity or incident light. The seasonal cycle described for Thalassia was positively correlated with temperature, whereas epiphyte standing crop was negatively correlated with water temperature.

An integrated approach to assess broad-scale condition of coastal wetlands—the Gulf of Mexico Coastal Wetlands pilot survey

The Environmental Protection Agency (EPA) and U.S. Geological Survey (USGS) initiated a two-year regional pilot survey in 2007 to develop, test, and validate tools and approaches to assess the condition of northern Gulf of Mexico (GOM) coastal wetlands. Sampling sites were selected from estuarine and palustrine wetland areas with herbaceous, forested, and shrub/scrub habitats delineated by the US Fish and Wildlife Service National Wetlands Inventory Status and Trends (NWI S&T) program and contained within northern GOM coastal watersheds. A multi-level, stepwise, iterative survey approach is being applied to multiple wetland classes at 100 probabilistically-selected coastal wetlands sites. Tier 1 provides information at the landscape scale about habitat inventory, land use, and environmental stressors associated with the watershed in which each wetland site is located. Tier 2, a rapid assessment conducted through a combination of office and field work, is based on best professional judgment and on-site evidence. Tier 3, an intensive site assessment, involves on-site collection of vegetation, water, and sediment samples to establish an integrated understanding of current wetland condition and validate methods and findings from Tiers 1 and 2. The results from this survey, along with other similar regional pilots from the Mid-Atlantic, West Coast, and Great Lakes Regions will contribute to a design and implementation approach for the National Wetlands Condition Assessment to be conducted by EPA’s Office of Water in 2011.

The ecological condition of south Florida estuaries

An assessment of the ecological condition ofsouth Florida estuaries based on regional probabilisticmonitoring was conducted during the summer of 1995. Samples anddata were collected on water and sediment quality, benthos, andfish tissue contaminants. Elevated concentrations of metals andpesticides were measured in both sediments and fish tissue withsome exceedances of guidance values. Bottom dissolved oxygenlevels over 23–37% of the area were below state criteria. Eighty-eight percent of surface waters had greater than10% penetration of ambient light to a depth of 1.0 m. Ninepercent of the area studied in South Florida exhibited degradedbiology and impaired use based on a calculated index ofecological condition. Using the probability-based monitoringdesign, useful information of this type can be provided toresource managers regarding estuarine condition on a regional scale.

Periphyton and Sediment Bioassessment in North Florida Bay

Periphyton colonization and sediment bioassessment were used ina survey to compare the relative environmental condition ofsampling sites located in Florida Bay and four peripheral sloughareas during the summer of 1995. Periphyton biomass, pigmentcontent, tissue quality and community composition weredetermined. In addition, benthic community composition and thetoxicities of whole sediments and associated pore waters weredetermined for two species of rooted macrophytes, an epibenthicinvertebrate and bioluminescent bacteria. Several locational differences were observed for the response parameters.Periphyton biomass was significantly greater in the Taylor Riverand the least in Shell Creek (P < 0.05). Most sediments were notacutely toxic to mysid shrimp nor phytotoxic. However, sedimentsfrom the Taylor River were more phytostimulatory than others (P< 0.05). Contaminant bioresidues were similar at most sites,however, mercury, chromium and nickel concentrations weregreater for periphyton colonized in the Taylor River and TroutCreek areas. Structural characteristics of the periphytic algalcommunity usually were statistically similar but a consistenttrend of lower density and diversity was evident for ShellCreek. The benthic community was the least diverse and dense inthe Canal C-111. The results of this study provide an initialindication of differences in the role of several slough areas aspossible sources of bioavailable contaminants to Florida Baywhich warrants additional investigation.

An assessment of water quality and primary productivity in Perdido Bay, a Northern Gulf of Mexico Estuary.

Perdido Bay is a shallow estuarine system of approximately 130 km2 with three major freshwater inputs. On a seasonal basis the productivity and chlorophyll a concentration of phytoplankton in Perdido Bay are controlled by temperature. One input, Eleven Mile Creek, is influenced by a paper mill discharge. Eleven Mile Creek exhibits high levels of light attenuation, high concentrations of dissolved nutrients, and low rates of carbon fixation that are significantly different from the other inputs or areas of Perdido Bay and productivity in Eleven Mile Creek is light limited. Upper Perdido Bay had slightly elevated concentrations of dissolved nutrients which correlate with significantly higher rates of carbon fixation and phytoplankton biomass. Nutrients and color from Eleven Mile Creck are diluted by the Perdido River inflow, restricting nutrient and light limitations to the area at the mouth of Eleven Mile Creek.

Effects of drilling fluids on Thalassia testudinum and its epiphytic algae

Abstract A flow-through microcosm system was developed to assess the potential influence of drilling fluids on Thalassia testudinum and its epiphytic algae. Two treatments (drilling fluid and a montmorillonite clay) and a control were used for seven tests: two 10-day, 200 μl/l exposures; two 10-day, 1000 μl/l; and three 6-week, 190 μl/l. Six-week exposure to drilling fluid reduced epiphyte biomass (measured as ash free dry weight/cm2), but surviving algae did not differ (measured as chlorophyll a/g epiphyte ash free dry weight) from controls. Thalassia productivity (carbon uptake and growth rate) was reduced by 10-day exposure to drilling fluid concentrations of 200 μl/l and 1000 μl/l. Thalassia productivity was reduced by drilling fluid exposure in summer and fall but not in spring. The variation in response is attributed to seasonal changes in Thalassia allotment and storage of carbohydrates. The effect of montmorillonite clay exposure varied inconsistently among all tests for both Thalassia and epiphytes.

Sediment toxicity and community composition of benthos and colonized periphyton in the Everglades-Florida Bay transitional zone.

This survey provides information on sediment toxicity and structural characteristics of the macrobenthic and periphytic algal communities at 10 locations in northeast Florida Bay. Whole sediments were not acutely toxic to Mysidopsis bahia (marine invertebrate) and Hyalella azteca (freshwater invertebrate) relative to reference sediment. Survival was between 80% and 100%. Community structure of the macrobenthos and algal-periphyton varied spatially. A total of 116 benthic species were identified at the 10 locations; mean density was greater in Shell Creek (10,017 organisms/m2) and least in Canal C-103 (441 organisms/m2). Tubificids and the crustacean Halmyrapseudes bahamensis (Family: Apseudidae) dominated the benthos at 4 of 10 locations. One hundred and six species of periphytic algae representing 52 genera were identified on substrates colonized for 21days. Mean algal density was greater in Florida Bay (19,440 cells/cm2) and least in Long Sound (10cells/cm2). Diatoms and blue green algae dominated the algal-periphyton. Major diatom genera were Navicula,Brachysira and Nitzschia. The more abundant and widely occurring blue–green taxa were species of Oscillatoria,Polycystis and Lyngbya. Ash free dry weight and chlorophyll a were significantly greater for periphyton colonized in Canal C-111 and Florida Bay and the least in Long Sound. Spatial variation and the availability of reference areas are important issues that need consideration in future biomonitoring efforts conducted in this region to ensure relevancy of results.

Sediment quality in near coastal waters of the Gulf of Mexico: Influence of Hurricane Katrina

The results of the present study represent a synoptic analysis of sediment quality in coastal waters of Lake Pontchartrain and Mississippi Sound two months after the landfall of Hurricane Katrina. Posthurricane conditions were compared to prehurricane (2000-2004) conditions, for sediment quality data. There were no exceedances of effects range median (ERM) sediment quality guideline values for chemical contaminants in any of the sediment samples collected from the Lake Pontchartrain or the Mississippi Sound study areas following the hurricane. Lower threshold effects range low (ERL) values were exceeded for As, Cd, and Ni at several stations in both survey areas, similar to levels of contamination observed prior to the hurricane. The comparison of sediment quality indicators before and after the hurricane suggests considerable stability of these systems with respect to short-term ecological impacts. Although other studies have shown storm-related changes could be detected (e.g., effects on benthic communities associated with shifts in salinity), there were no indications of widespread sediment contamination.