José Carlos Albuquerque do Prado Carvalho

Campylobacter jejuni/coli : Methodology of isolation and possible interfering factors in primary culture

The prevalence of Campylobacter jejuni and Campylobacter coli was investigated in 64 samples of fresh retail chicken purchased from commercial slaughterhouses located in Brazil. Campylobacter spp. were isolated from 40 (62.5%) of 64 analyzed samples. The strains biotyped according to Lior were classified as C. jejuni biotypes I and II, and C. coli biotypes I and II. The efficiency of different procedures for recovering Campylobacter spp. from chicken carcasses was tested. The enrichment procedure was significantly less effective than direct plating (P < 0.05), detecting 19 of 40 (47.5%) as opposed to 38 of 40 (95%) positive samples. Using direct plating the efficiency of Blasers selective supplement was significantly more effective (P < 0.05) than Skirrows selective supplement. To verify which factors could be affecting Campylobacter spp. growth in enrichment broth, the pH was measured after incubation for 48 h at 42°C and lactobacilli, coliforms, and enterococci were enumerated. Most of the Campylobacter -negative samples presented high levels of indicator microorganisms, which may have hindered the recovery of Campylobacter spp. during the enrichment procedure.

Avaliação bacteriológica de carne suína (carré = "Longissimus dorsi +base óssea") comercializada em Niterói e São Gonçalo, Estado do Rio de Janeiro, Brasil

O presente trabalho procurou avaliar as condicoes higienico-sanitarias de como e comercializada a carne suina(carre) em Niteroi e Sao Goncalo, Estado do Rio de Janeiro.Foram adquiridas 30 amostras de carne suina emdiversos acougues dos municipios citados e submetidasas seguintes analises bacteriologicas: contagem de bacteriasheterotroficas aerobias mesofilas viaveis; contagemde bacterias heterotroficas psicrotroficas viaveis; enumeracaode coliformes fecais; isolamento e identificacao deEscherichia co/i e contagem, isolamento e identificacaode Staphylococcus aureus. Os resultados obtidos foramconfrontados com os padroes vigentes da legislacao nacionale/ou com aqueles sugeridos por autores da bibliografiaconsultada. Na contagem de bacterias mesofila viaveis1 O amostras (33,3%) apresentaram valores superioresa 1 os UFCs/g; na contagem de bacteriaspsicrotroficas viaveis 8 amostras (26,6%) apresentaramvalores superiores a 1 06 UFCs/g que, segundo os PadroesBacteriologicos dos Alimentos Portugueses citados emRibeiro(1974), teriam extrapolado os limites maximos de1 os UFCs/g e 106 UFCs/g respectivamente. Para a contagemde coliformes fecais e E. co/i 11 amostras (36, 7%)foram classificadas como produto improprio para consumosegundo a portaria 001 da Divisao Nacional de Alimentosde 1987 (Brasil, 1987). Com relacao a contageme identificacao de S. aureus duas amostras (6, 7%) estariamcondenadas para o consumo por conterem contagensacima do estabeleciado pela DINAL (Brasil, 1987).