Junken Aoki
Japan Agency for Medical Research and Development
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Publication
Featured researches published by Junken Aoki.
Journal of Biological Chemistry | 2017
Yusuke Hirata; Miki Takahashi; Yuki Kudoh; Kuniyuki Kano; Hiroki Kawana; Kumiko Makide; Yasuharu Shinoda; Yasushi Yabuki; Kohji Fukunaga; Junken Aoki; Takuya Noguchi; Atsushi Matsuzawa
Food-borne trans-fatty acids (TFAs) are mainly produced as byproducts during food manufacture. Recent epidemiological studies have revealed that TFA consumption is a major risk factor for various disorders, including atherosclerosis. However, the underlying mechanisms in this disease etiology are largely unknown. Here we have shown that TFAs potentiate activation of apoptosis signal-regulating kinase 1 (ASK1) induced by extracellular ATP, a damage-associated molecular pattern leaked from injured cells. Major food-associated TFAs such as elaidic acid (EA), linoelaidic acid, and trans-vaccenic acid, but not their corresponding cis isomers, dramatically enhanced extracellular ATP-induced apoptosis, accompanied by elevated activation of the ASK1-p38 pathway in a macrophage-like cell line, RAW264.7. Moreover, knocking out the ASK1-encoding gene abolished EA-mediated enhancement of apoptosis. We have reported previously that extracellular ATP induces apoptosis through the ASK1-p38 pathway activated by reactive oxygen species generated downstream of the P2X purinoceptor 7 (P2X7). However, here we show that EA did not increase ATP-induced reactive oxygen species generation but, rather, augmented the effects of calcium/calmodulin-dependent kinase II-dependent ASK1 activation. These results demonstrate that TFAs promote extracellular ATP-induced apoptosis by targeting ASK1 and indicate novel TFA-associated pathways leading to inflammatory signal transduction and cell death that underlie the pathogenesis and progression of TFA-induced atherosclerosis. Our study thus provides insight into the pathogenic mechanisms of and proposes potential therapeutic targets for these TFA-related disorders.
Archive | 2012
Junken Aoki; 青木 淳賢; Kumiko Makide; 久美子 巻出; Asuka Inoue; 飛鳥 井上; Tomohiko Ohwada; 智彦 大和田; Masaya Ikubo; 仁也 井久保; Yuko Otani; 優子 尾谷
The Japanese Biochemical Society/The Molecular Biology Society of Japan | 2017
Kouki Kawakami; Asuka Inoue; Takeaki Shibata; Junken Aoki
The Japanese Biochemical Society/The Molecular Biology Society of Japan | 2017
Takumi Sugawara; Kuniyuki Kano; Junken Aoki
The Japanese Biochemical Society/The Molecular Biology Society of Japan | 2017
Ryoji Kise; Yuji Shinjo; Kuniyuki Kano; Asuka Inoue; Junken Aoki
The Japanese Biochemical Society/The Molecular Biology Society of Japan | 2017
Kairn Itoh; Masaki Tsuchiya; Kohjiro Nagao; Junken Aoki; Tomohiko Ohwada; Yasunori Takayama; Makoto Tominaga; Yuji Hara; Masato Umeda
The Japanese Biochemical Society/The Molecular Biology Society of Japan | 2017
Takayuki Kishi; Asuka Inoue; Junken Aoki
The Japanese Biochemical Society/The Molecular Biology Society of Japan | 2017
Naoki Omae; Hitomi Oto; Kuniyuki Kano; Junken Aoki
The Japanese Biochemical Society/The Molecular Biology Society of Japan | 2017
Ryohei Okasato; Ryoji Kise; Kuniyuki Kano; Atsuo Kawahara; Junken Aoki
The Japanese Biochemical Society/The Molecular Biology Society of Japan | 2017
Yuki Ono; Asuka Inoue; Satoru Ishida; Kouki Kawakami; Junken Aoki