Karen Harper

Effect of probiotic Bacillus amyloliquefaciens strain H57 on productivity and the incidence of diarrhoea in dairy calves

A spore-forming probiotic, Bacillus amyloliquefaciens strain H57 (H57), was administered to dairy calves in starter pellets to determine effects on liveweight gain, feed conversion efficiency and animal health under summer feeding conditions, without antibiotics. Twenty-four male and female calves were allocated into two groups and from 4 weeks of age individually offered 6 L/day of whole milk and ad libitum starter pellets impregnated with H57 (3.16 × 108 cfu per kg DM) or without (Control) until 12 weeks of age. The calves were housed in a non-air-conditioned animal house, with deep-straw bedding over concrete, under typically challenging subtropical summer conditions. After 12 weeks the calves were released into a grazing paddock as one group and were supplemented ad libitum with control pellets and hay, until 19 weeks of age. From Weeks 4 to 12, liveweight and feed intakes were measured weekly and health status was monitored daily. Rumen fluid and blood were collected at Weeks 4 and 12, and to test for persistence after cessation of feeding H57, each were measured again at Week 19. From Weeks 4 to 12, the H57 calves grew faster (767 vs 551 g/day, P = 0.01), tended to consume more pellets (1013 vs 740 g DM/day, P = 0.07) and were 19% more feed conversion efficiency (2.43 vs 2.90 kg milk + starter DM/kg weight gain, P = 0.01) compared with the Control calves. The mean duration of each diarrhoea event was 2 days less for the H57 calves than the Control (P = 0.01). The H57 calves weaned 9 days earlier (P = 0.02) and were heavier at Week 19 (155 vs 139 kg, P = 0.03) than the Control calves. The only effect of H57 on rumen volatile fatty acid concentrations was an elevation in valerate at Week 12 (4.10 vs 2.47 mmol/L, P = 0.03). Plasma β-hydroxy butyrate was also elevated in the H57 calves at Week 19 (0.24 vs 0.20 mmol/L), indicating the potential of H57 to improve rumen development. H57 can be used to improve the nutritional performance and reduce the risk of diarrhoea in dairy calves as they transition from milk to dry feed.

Effect of time and diet change on the bacterial community structure throughout the gastrointestinal tract and in faeces of the northern brown bandicoot, Isoodon macrourus

Abstract. A significant gap, in not only peramelid nutritional physiology but marsupial nutrition as a whole, is the lack of information relating to microorganisms of the gastrointestinal tract. This research is a preliminary investigation that will provide a baseline for comparisons among peramelids. The high degree of 16S rRNA gene clones identified in this research that are closely related to culturable bacteria suggests that additional research will enable a more complete description of the gastrointestinal bacteria of I. macrourus. Most identifiable clones belonged to Clostridium and Ruminococcus. This research has confirmed that the hindgut of I. macrourus, the caecum, proximal colon and distal colon, are the main sites for microbial activity.