Kathryn Page

A review of sampling designs for the measurement of soil organic carbon in Australian grazing lands

The accurate measurement of the soil organic carbon (SOC) stock in Australian grazing lands is important due to the major role that SOC plays in soil productivity and the potential influence of soil C cycling on Australia’s greenhouse gas emissions. However, the current sampling methodologies for SOC stock are varied and potentially conflicting. It was the objective of this paper to review the nature of, and reasons for, SOC variability; the sampling methodologies commonly used; and to identify knowledge gaps for SOC measurement in grazing lands. Soil C consists of a range of biological materials, in various SOC pools such as dissolved organic C, micro- and meso-fauna (microbial biomass), fungal hyphae and fresh plant residues in or on the soil (particulate organic C, light-fraction C), the products of decomposition (humus, slow pool C) and complexed organic C, and char and phytoliths (inert, passive or resistant C); and soil inorganic C (carbonates and bicarbonates). Microbial biomass and particulate or light-fraction organic C are most sensitive to management or land-use change; resistant organic C and soil carbonates are least sensitive. The SOC present at any location is influenced by a series of complex interactions between plant growth, climate, soil type or parent material, topography and site management. Because of this, SOC stock and SOC pools are highly variable on both spatial and temporal scales. This creates a challenge for efficient sampling. Sampling methods are predominantly based on design-based (classical) statistical techniques, crucial to which is a randomised sampling pattern that negates bias. Alternatively a model-based (geostatistical) analysis can be used, which does not require randomisation. Each approach is equally valid to characterise SOC in the rangelands. However, given that SOC reporting in the rangelands will almost certainly rely on average values for some aggregated scale (such as a paddock or property), we contend that the design-based approach might be preferred. We also challenge soil surveyors and their sponsors to realise that: (i) paired sites are the most efficient way of detecting a temporal change in SOC stock, but destructive sampling and cumulative measurement errors decrease our ability to detect change; (ii) due to (i), an efficient sampling scheme to estimate baseline status is not likely to be an efficient sampling scheme to estimate temporal change; (iii) samples should be collected as widely as possible within the area of interest; (iv) replicate of laboratory analyses is a critical step in being able to characterise temporal change. Sampling requirements for SOC stock in Australian grazing lands are yet to be explicitly quantified and an examination of a range of these ecosystems is required in order to assess the sampling densities and techniques necessary to detect specified changes in SOC stock and SOC pools. An examination of techniques that can help reduce sampling requirements (such as measurement of the SOC fractions that are most sensitive to management changes and/or measurement at specific times of the year – preferably before rapid plant growth – to decrease temporal variability), and new technologies for in situ SOC measurement is also required.

Organic carbon stocks in cropping soils of Queensland, Australia, as affected by tillage management, climate, and soil characteristics

Research both nationally and internationally has indicated that no-till (NT) management used in combination with stubble retention has the potential to increase soil organic carbon (SOC) stocks in cropping soils relative to conventional tillage (CT). However, rates of SOC increase can vary depending on cropping system, climate, and soil type, making the quantification of carbon change difficult on a regional level. Various long-term trials and commercial sites throughout Queensland were used to compare rates of SOC change under CT and NT management in cropping soils, and to determine how climate and soil type interact to influence rates of change. It was observed that NT management was not capable of increasing SOC stocks under the crop–fallow rotation systems practised throughout Queensland, and was unlikely even to hold SOC stocks steady under current management practices. However, SOC losses under NT systems did appear to be slower than under CT, indicating that NT may slow SOC loss following a period of organic carbon input, for example, from a pasture ley. On a regional scale, biomass production (estimated through remote sensing), climate (specifically the vapour pressure deficit), and soil sand content could be used to adequately predict SOC stocks on commercial sites, indicating the importance of considering these factors when assessing SOC stocks following management change across the region.

Processes and magnitude of CO2, CH4, and N2O fluxes from liming of Australian acidic soils: a review.

Increases in soil acidification have led to large increases in the application of aglime to Australian agricultural soils. The addition of aglime has the potential to increase greenhouse gas (GHG) emissions due to the release of CO2 during the chemical dissolution of aglime and due to pH-induced changes to soil biological processes. Currently, Australia’s GHG accounting system assumes that all the carbon contained in aglime is released to the atmosphere during dissolution in accordance with the Tier 1 methodology of the IPCC. However, a recent approach by TO West and AC McBride has questioned this assumption, hypothesising that a proportion of the carbon from riverine-transported aglime may be sequestered in seawater. In addition, there is presently no capacity within Australia’s carbon accounting system to quantify changes to GHG emissions from lime-induced changes to soil biological processes. Therefore, the primary objective of this review was to examine the chemical and biological processes occurring during the application of aglime and the subsequent fluxes in CO2, N2O, and CH4 from soil, with particular reference to the Australian environment. Estimates for CO2 emissions from aglime application in Australia using the contrasting methodologies of the IPCC and West and McBride were compared. Using the methodology of the IPCC it was determined that from the aglime applied in Australia in 2002, 0.995 Tg of CO2 would have been emitted, whereas this figure was reduced to 0.659–0.860 Tg of CO2 using the methodology of West and McBride. However, the accuracy of these estimates is currently limited by poor understanding of the manner in which aglime moves within the Australian landscapes. In addition, there are only a very small number of Australian studies that have examined the effect of aglime on GHG emissions due to changes in soil biological processes, limiting the ability of Australian modellers to accurately incorporate these processes within the carbon accounting system.

Climate and soil properties limit the positive effects of land use reversion on carbon storage in Eastern Australia

Australia’s “Direct Action” climate change policy relies on purchasing greenhouse gas abatement from projects undertaking approved abatement activities. Management of soil organic carbon (SOC) in agricultural soils is an approved activity, based on the expectation that land use change can deliver significant changes in SOC. However, there are concerns that climate, topography and soil texture will limit changes in SOC stocks. This work analyses data from 1482 sites surveyed across the major agricultural regions of Eastern Australia to determine the relative importance of land use vs. other drivers of SOC. Variation in land use explained only 1.4% of the total variation in SOC, with aridity and soil texture the main regulators of SOC stock under different land uses. Results suggest the greatest potential for increasing SOC stocks in Eastern Australian agricultural regions lies in converting from cropping to pasture on heavy textured soils in the humid regions.

Changes in total soil organic carbon stocks and carbon fractions in sugarcane systems as affected by tillage and trash management in Queensland, Australia

The use of sugarcane trash (tops and residue) retention systems has been reported to lead to increases in total soil organic carbon (TOC) stocks. However, these increases have generally been small and confined to the top 0.05 m of the soil profile. It has been hypothesised that the amount of TOC sequestered could be increased if the intensive tillage that occurs at the end of a sugarcane ratoon cycle, which is known to decrease TOC, could be eliminated. This research examined the effect of no-till management and/or trash retention on four trial sites throughout Queensland, to assess the ability of this management to increase TOC stocks. Management effects on particulate organic carbon (POC), humus organic carbon (HOC), and resistant organic carbon (ROC) stocks were also assessed using mid-infrared spectroscopy. No significant changes in TOC, POC, HOC, or ROC were observed over either 0–0.1 or 0–0.3 m depth at any of the sites examined, when sites were considered as a whole. The results indicate that these management practices currently have limited capacity to increase TOC stocks on these soil types over 0–0.1 or 0–0.3 m depth for the purposes of carbon sequestration.

Nitrate ammonification and its relationship to the accumulation of ammonium in a Vertisol subsoil

High concentrations of ammonium ( up to 270 kg N/ha) have been observed in a Vertisol soil below 1 m depth near Warra in south-east Queensland. This study examined the possibility that increased water movement into the subsoil after the removal of native vegetation, and a subsequent increase in periods of waterlogging, could have triggered nitrate ammonification and be responsible for the production of ammonium. Two incubation experiments were conducted to test this hypothesis. The first involved the incubation of repacked cores that had been amended with 30 mg N/kg of 5 atom% N-15 nitrate under low oxygen conditions for a period of 360 days. Over this time period the N-15 enrichment of the exchangeable ammonium fraction was monitored in order to detect any reduction of nitrate to ammonium. The second experiment involved the incubation of soil amended with 30 mg N/ kg of 5 atom% N-15 nitrate under waterlogged and low oxygen conditions for 75 days. During this period the redox potential of the soil was monitored using a field test to determine if reducing conditions would develop in this soil over a period of waterlogging, combined with the monitoring of any nitrate reduction to ammonium. The results of these experiments indicated that a small amount of nitrate ammonification (< 0.1 mg N/ kg) could be observed in the Warra subsoil, but that unless the rate of reduction were to significantly increase with time, this could not account for the accumulation of ammonium observed in the field. The environmental conditions that would make either dissimilatory or abiotic nitrate ammonification favourable were not observed to develop. Consequently, it has been concluded that the observed nitrate ammonification occurred via an assimilatory pathway. Due to the low rate of microbial activity in this subsoil it is considered unlikely that this process was responsible for the subsoil ammonium accumulation at Warra.

Using quantity/intensity relationships to assess the potential for ammonium leaching in a Vertosol

High concentrations of ammonium (up to 0.1 cmol/kg) have been observed below 1 m depth in a Vertosol soil near Warra in south-eastern Queensland. This study examined whether ammonium leaching could be responsible for the ammonium accumulation observed in the Warra soil. This was done by using quantity/intensity (Q/I) relationships to compare the ammonium retention capacity of the Warra soil with other similar soils throughout the region that did not contain elevated subsoil ammonium concentrations. Analysis of Q/I curves revealed that in the concentration range studied, the amount of ammonium retained on high affinity adsorption sites in all 3 soils was low, and the Warra soil was not significantly different from the other 2 soils. The ability of the soils to retain ammonium in the soil solution against leaching [i.e. their potential buffer capacity (PBC)] did differ between soils and was greatest at Warra. This indicates that at any one time the Warra soil holds more ammonium on the exchange complex and less in solution than the other soils examined. It was concluded that ammonium is no more likely to leach through the surface horizons of the Warra soil than the other soils examined. Indeed, the data indicated that the Warra soil probably has greater capacity to retain ammonium against leaching due to its greater PBC. Consequently, it is considered unlikely that leaching of ammonium has been a major contributor to the subsoil ammonium concentrations at Warra.

Subsoil nitrogen mineralisation and its potential to contribute to NH4 accumulation in a Vertosol

High concentrations of NH4+ (up to 270 kg N/ha) have been observed in a Vertosol below 1 m depth in south-east Queensland. This study examined the possibility that mineralisation associated with the removal of native vegetation (Acacia harpophylla) for cropping was responsible for the production of NH4+. Particularly, the potential contribution of decomposing root material and/or dissolved organic nitrogen (DON) leached into the subsoil after clearing was investigated. The amount of N that was contained within native vegetation root material was determined from an area of native vegetation adjacent to the cleared site containing elevated NH4+ concentrations. In addition, the amount of NH4+ that could be mineralised in the native vegetation soil was determined by monitoring NH4+ concentrations over 360 days in intact cores, and by conducting waterlogged incubations. To determine the rate at which a source of DON leached into the subsoil would mineralise, soil was amended with glutamic acid at a rate of 250 mg N/kg and placed under waterlogged incubation. The possibility that the acidic pH of the subsoil, or the lack of a significant subsoil microbial population, was inhibiting mineralisation was also examined by increasing soil pH from 4.4 to 7.0, and inoculating the subsoil with surface soil microorganisms during waterlogged incubations. Low concentrations of N, approximately 90 kg N/ha between 1.2 and 3 m, were found in the native vegetation root material. In addition, no net N mineralisation was observed in either the extended incubation of intact cores or in the control samples of the waterlogged incubations. Net N mineralisation was also not detected when the subsoil was amended with a source of organic N. Results indicate that this lack of mineralisation is largely due to pH inhibition of the microbial population. It is concluded that the mineralisation of either in situ organic material, or DON transported to the subsoil during leaching events, is unlikely to have significantly contributed to the subsoil NH4 accumulation at the study site.

How useful are MIR predictions of total, particulate, humus, and resistant organic carbon for examining changes in soil carbon stocks in response to different crop management? A case study

Measures of particulate organic carbon (POC), humus organic carbon (HOC), and resistant organic carbon (ROC) (primarily char) are often used to represent the active, slow, and inert carbon pools used in soil carbon models. However, these fractions are difficult to measure directly, and mid infrared (MIR) spectroscopic techniques are increasingly being investigated to quantify these fractions and total organic carbon (TOC). This study examined the change in MIR-predicted pools of TOC, POC, HOC, and ROC in response to different crop management between two time periods (1981 and 2008) in a long-term wheat cropping trial in Queensland, Australia. The aims were (i) to assess the ability of MIR to detect changes in carbon stocks compared with direct measurements of TOC (LECO-TOC); and (ii) to assess how well the behaviour of POC, HOC, and ROC corresponded with the active, slow, and inert conceptual carbon pools. Significant declines in carbon stocks were observed over time using both LECO-TOC and MIR-predicted stocks of TOC, POC, HOC, and ROC, although MIR-TOC under-estimated loss by 27–30% compared with LECO-TOC. The decline in MIR-POC and MIR-HOC was consistent with the expected behaviour of the active and slow conceptual pools; however, the decline in ROC was not consistent with that of the inert pool. In addition, MIR measurements did not accurately detect differences in the rate of carbon loss under different crop management practices.

Native forest management and greenhouse gas emissions – how much do we really know?

The modelling system currently used to calculate emissions and sequestrations from Australian forestry operations – FullCAM – is underpinned by a great deal of research. However, certain aspects of forestry operations, including harvesting within native forests, are yet to be fully incorporated into this system. Recently, researchers from Queensland Department of Environment and Resource Management and CSIRO reviewed current knowledge of changes to GHG emissions and carbon cycling following harvesting in Australian native forests, and identified areas requiring further research. ‘Off site’ GHG emissions from harvested products (e.g. when wood waste is sent to landfill) were not considered in the review.