Kathy Burns

Organochlorine Pesticide Residues in Soils and Sediments of the Herbert and Burdekin River Regions, North Queensland – Implications for Contamination of the Great Barrier Reef

Organochlorine pesticides were widely used in the Australian sugarcane industry from the early 1950s until the late 1980s. Erosion of sugarcane soils and subsequent transport of sediment bound contaminants in river run-off to the Great Barrier Reef lagoon is a growing concern as the cane industry continues to expand. Organochlorine pesticide residues can be used as tracers to examine the worst-case scenario of the spatial extent to which currently used, though less persistent, organic agricultural pesticides might extend. The coastal alluvial flood-plains of the Herbert and Burdekin Rivers in North Queensland have sugarcane growing as the major coastal land-use. Sediment cores and surface sediment samples were collected from near-shore coastal regions of the Herbert and Burdekin Rivers. In addition, soil samples from cane-fields in the two catchments were collected. Analyses of the marine surface sediment samples and three sediment cores revealed the absence of detectable concentrations of organochlorine pesticides (<5 pg/g). However, easily detectable concentrations were found in the sugarcane soil samples (0.01–45 ng/g).

Dispersion and fate of produced formation water constituents in an Australian Northwest Shelf shallow water ecosystem

This was a study of produced formation water (PFW) discharged into a shallow tropical marine ecosystem on the Northwest Shelf of Australia. A combination of oceanographic techniques, geochemical tracer studies, chemical and biological assessment methods, and dispersion modelling was used to describe the distribution and fate of petroleum hydrocarbons and added nutrients discharged from an offshore production platform. Using fine scale volatile hydrocarbon data, the horizontal and vertical diffusion parameters for a three dimensional dispersion model were calibrated under local conditions. Trace hydrocarbon chemistry studies and integration of the data into a mass balance model, facilitated a comprehensive description of dispersion and degradation pathways and rates. Bio-accumulation into bivalves and water column microbial growth inhibition studies confirmed the chemistry and model predictions that the area of potential biological impact extended to 0.5 nautical miles (∼900 m) from the discharge with additional skewing in the direction of the predominant tidal flows. Impact would be expected to be concentrated in transient surface slicks and near surface seawater. Dispersion and degradation processes were fast enough to prevent any long-term build-up of contamination within the system. Trace levels of oil in the near field sandy sediments were directly related to the magnitude of the daily discharge. The study is a benchmark to help predict the effects of further oil industry expansion in this pristine coastal region.

Assessing the oil degradation potential of endogenous micro‐organisms in tropical marine wetlands

As part of a larger study on the bioremediation of oil spills in tropical mangrove habitats, we conducted a series of flask experiments to test for the presence of hydrocarbon degrading micro‐organisms in representative wetland habitats. Also tested was the biodegradation of selected oils (Gippsland Crude, Arabian Light Crude and Bunker C), that are transported along the Australian coast. We also tested for potential inhibition of biodegradation by natural organics in the mangrove pore waters and evaluated the ability of an oxygen release compound (ORC) to stimulate biodegradative processes. Evaporation was a significant factor in removing the light alkane and aromatic hydrocarbons from air and nitrogen sparged flasks. Evaporation removed ∼27% of the Gippsland, ∼37%of the Arabian, and ∼10% of the Bunker oils. Oxygen was necessary to support biodegradation as expected. The micro‐organisms were capable of biodegrading the non‐volatile saturate fraction of each oil. Degradation removed another 14 of the Gippsland, 30 of the Arabian, and 22 of the Bunker C oils. Normalisation of the individual aromatic hydrocarbon classes to internal triterpane biomarkers indicated some degradation of aromatics in the Arabian Light and Bunker C oils. Although alkane degradation rates were comparable in the three oils, the Gippsland oil had a higher wax content and after 14 days incubation, still contained as much as 25 of the alkanes present in the original oil. Thus, degradation of its aromatic fraction may have been delayed. Based on these results we estimate that Arabian Light Crude oil would have a shorter residence time than the other oils in mangrove sediment. It has a higher content of light hydrocarbons, which are readily removed by both physical and microbial processes. The Bunker C would be expected to have the longest residence time in mangrove sediment, because it contains a larger percentage of higher molecular weight, unresolved components. Comparison of the efficiency of inoculates from three tropical intertidal habitats (Avicennia and Rhizophora mangroves, plus salt marsh sediments) indicated the presence of hydrocarbon degrading micro‐organisms in all three habitats. There was no known history of oil contamination in the soil source area. There was no inhibition of degradation due to addition of mangrove pore waters. The ORC did not facilitate degradation in closed laboratory experiments. These results were used to formulate a bioremediation strategy to treat oiled sediments in mangrove forests in Queensland Australia, which was based on forced aeration and nutrient addition.Evaporation was a significant factor in removing the light alkane and aromatic hydrocarbons from air and nitrogen sparged flasks. Evaporation removed ∼27% of the Gippsland, ∼37% of the Arabian, and ∼10% of the Bunker oils. Oxygen was necessary to support biodegradation as expected. The micro-organisms were capable of biodegrading the non-volatile saturate fraction of each oil. Degradation removed another 14% of the Gippsland, 30% of the Arabian, and 22% of the Bunker C oils. Normalisation of the individual aromatic hydrocarbon classes to internal triterpane biomarkers indicated some degradation of aromatics in the Arabian Light and Bunker C oils. Although alkane degradation rates were comparable in the three oils, the Gippsland oil had a higher wax content and after 14 days incubation, still contained as much as 25% of the alkanes present in the original oil. Thus, degradation of its aromatic fraction may have been delayed. Based on these results we estimate that Arabian Light Crude oil would have a shorter residence time than the other oils in mangrove sediment. It has a higher content of light hydrocarbons, which are readily removed by both physical and microbial processes. The Bunker C would be expected to have the longest residence time in mangrove sediment, because it contains a larger percentage of higher molecular weight, unresolved components. Comparison of the efficiency of inoculates from three tropical intertidal habitats (Avicennia and Rhizophora mangroves, plus salt marsh sediments) indicated the presence of hydrocarbon degrading micro-organisms in all three habitats. There was no known history of oil contamination in the soil source area. There was no inhibition of degradation due to addition of mangrove pore waters. The ORC did not facilitate degradation in closed laboratory experiments.These results were used to formulate a bioremediation strategy to treat oiled sediments in mangrove forests in Queensland Australia, which was based on forced aeration and nutrient addition.