Susan Codi

Gladstone, Australia Field Studies: Weathering and Degradation of Hydrocarbons in Oiled Mangrove and Salt Marsh Sediments With and Without the Application of an Experimental Bioremediation Protocol

This field study was a combined chemical and biological investigation of the relative rates of weathering and biodegradation of oil spilled in sediments and testing the influence of a bioremediation protocol. The aim of the chemistry work presented here was to determine whether the bioremediation protocol affected the rate of penetration, dissipation or long-term retention of a medium range crude oil (Gippsland) and a Bunker C oil stranded in tropical Rhizophora sp. mangrove and Halosarcia sp. salt marsh environments. Permission for the planned oil spills was granted in the Port Authority area of Gladstone, Queensland (Australia). Sediment cores from three replicate plots of each treatment for mangroves and four replicate plots for the salt marsh (oil only and oil plus bioremediation) were analysed for total hydrocarbons (THC) and for individual alkane markers using gas chromatography with flame ionization detection (GC-FID). Sediments were collected at day 2, then 1, 2, 5 or 6 and 12 or 13 months post-spill for mangroves and day 2, 1, 3 and 9 months post-spill for salt marshes. Over this time, hydrocarbons in all of the oil treated plots decreased exponentially, There was no statistical difference in initial oil concentrations, penetration of oil to depth, or in the rates of oil dissipation between untreated oil and bioremediated oil in the mangrove plots. The salt marsh plots treated with the waxy Gippsland oil showed a faster rate of biodegradation of the oil in the bioremediated plots. In this case only, the degradation rate significantly impacted the mass balance of remaining oil. The Bunker C oil contained only minor amounts of highly degradable il-alkanes and bioremediation did not significantly impact its rate of loss in the salt marsh sediments, At the end of each experiment, there were still n-alkanes visible in the gas chromatograms of residual oils. Thus it was concluded that there was unlikely to be any change in the stable internal biomarkers of the oils over this time period. The predominant removal processes in both habitats were evaporation and dissolution, with a lag-phase of 1-2 months before the start of microbial degradation

Dispersion and fate of produced formation water constituents in an Australian Northwest Shelf shallow water ecosystem

This was a study of produced formation water (PFW) discharged into a shallow tropical marine ecosystem on the Northwest Shelf of Australia. A combination of oceanographic techniques, geochemical tracer studies, chemical and biological assessment methods, and dispersion modelling was used to describe the distribution and fate of petroleum hydrocarbons and added nutrients discharged from an offshore production platform. Using fine scale volatile hydrocarbon data, the horizontal and vertical diffusion parameters for a three dimensional dispersion model were calibrated under local conditions. Trace hydrocarbon chemistry studies and integration of the data into a mass balance model, facilitated a comprehensive description of dispersion and degradation pathways and rates. Bio-accumulation into bivalves and water column microbial growth inhibition studies confirmed the chemistry and model predictions that the area of potential biological impact extended to 0.5 nautical miles (∼900 m) from the discharge with additional skewing in the direction of the predominant tidal flows. Impact would be expected to be concentrated in transient surface slicks and near surface seawater. Dispersion and degradation processes were fast enough to prevent any long-term build-up of contamination within the system. Trace levels of oil in the near field sandy sediments were directly related to the magnitude of the daily discharge. The study is a benchmark to help predict the effects of further oil industry expansion in this pristine coastal region.

Assessing the oil degradation potential of endogenous micro‐organisms in tropical marine wetlands

As part of a larger study on the bioremediation of oil spills in tropical mangrove habitats, we conducted a series of flask experiments to test for the presence of hydrocarbon degrading micro‐organisms in representative wetland habitats. Also tested was the biodegradation of selected oils (Gippsland Crude, Arabian Light Crude and Bunker C), that are transported along the Australian coast. We also tested for potential inhibition of biodegradation by natural organics in the mangrove pore waters and evaluated the ability of an oxygen release compound (ORC) to stimulate biodegradative processes. Evaporation was a significant factor in removing the light alkane and aromatic hydrocarbons from air and nitrogen sparged flasks. Evaporation removed ∼27% of the Gippsland, ∼37%of the Arabian, and ∼10% of the Bunker oils. Oxygen was necessary to support biodegradation as expected. The micro‐organisms were capable of biodegrading the non‐volatile saturate fraction of each oil. Degradation removed another 14 of the Gippsland, 30 of the Arabian, and 22 of the Bunker C oils. Normalisation of the individual aromatic hydrocarbon classes to internal triterpane biomarkers indicated some degradation of aromatics in the Arabian Light and Bunker C oils. Although alkane degradation rates were comparable in the three oils, the Gippsland oil had a higher wax content and after 14 days incubation, still contained as much as 25 of the alkanes present in the original oil. Thus, degradation of its aromatic fraction may have been delayed. Based on these results we estimate that Arabian Light Crude oil would have a shorter residence time than the other oils in mangrove sediment. It has a higher content of light hydrocarbons, which are readily removed by both physical and microbial processes. The Bunker C would be expected to have the longest residence time in mangrove sediment, because it contains a larger percentage of higher molecular weight, unresolved components. Comparison of the efficiency of inoculates from three tropical intertidal habitats (Avicennia and Rhizophora mangroves, plus salt marsh sediments) indicated the presence of hydrocarbon degrading micro‐organisms in all three habitats. There was no known history of oil contamination in the soil source area. There was no inhibition of degradation due to addition of mangrove pore waters. The ORC did not facilitate degradation in closed laboratory experiments. These results were used to formulate a bioremediation strategy to treat oiled sediments in mangrove forests in Queensland Australia, which was based on forced aeration and nutrient addition.Evaporation was a significant factor in removing the light alkane and aromatic hydrocarbons from air and nitrogen sparged flasks. Evaporation removed ∼27% of the Gippsland, ∼37% of the Arabian, and ∼10% of the Bunker oils. Oxygen was necessary to support biodegradation as expected. The micro-organisms were capable of biodegrading the non-volatile saturate fraction of each oil. Degradation removed another 14% of the Gippsland, 30% of the Arabian, and 22% of the Bunker C oils. Normalisation of the individual aromatic hydrocarbon classes to internal triterpane biomarkers indicated some degradation of aromatics in the Arabian Light and Bunker C oils. Although alkane degradation rates were comparable in the three oils, the Gippsland oil had a higher wax content and after 14 days incubation, still contained as much as 25% of the alkanes present in the original oil. Thus, degradation of its aromatic fraction may have been delayed. Based on these results we estimate that Arabian Light Crude oil would have a shorter residence time than the other oils in mangrove sediment. It has a higher content of light hydrocarbons, which are readily removed by both physical and microbial processes. The Bunker C would be expected to have the longest residence time in mangrove sediment, because it contains a larger percentage of higher molecular weight, unresolved components. Comparison of the efficiency of inoculates from three tropical intertidal habitats (Avicennia and Rhizophora mangroves, plus salt marsh sediments) indicated the presence of hydrocarbon degrading micro-organisms in all three habitats. There was no known history of oil contamination in the soil source area. There was no inhibition of degradation due to addition of mangrove pore waters. The ORC did not facilitate degradation in closed laboratory experiments.These results were used to formulate a bioremediation strategy to treat oiled sediments in mangrove forests in Queensland Australia, which was based on forced aeration and nutrient addition.

Contrasting impacts of localised versus catastrophic oil spills in mangrove sediments

Several truckloads of mixed waste oil were dumped onto a short section of road and into the intertidal wetlands near Cairns, Queensland in January, 1994. The oil contaminated a band of mangroves 15–44 m wide along approximately 200 m of road. Impacted marsh included Melaleuca forest and high-intertidal mangroves. The initial concentrations of petroleum hydrocarbons in surface sediments reached 17% of the dry weight in heavily impacted areas. These high concentrations observed in limited spatial areas were similar to those observed over large spatial areas after a catastrophic oil spill in Panama in 1986. No large scale biological damage was observed from this localised spill. Clean up efforts and natural dissipation processes reduced sediment hydrocarbon loads to non-acutely toxic levels in 1.5 years in the intertidal mangroves. High hydrocarbon concentrations remained in the Melaleuca sediments for at least two years post spill. Internal molecular markers were used to detail hydrocarbon dissipation and degradation rates. This study provides a contrast between impacts of localised versus catastrophic oil spills in tropical mangrove habitats.

Identifying sources of organic matter in sediments from a detritivorous coral reef fish territory

Sediment and filamentous algae were collected from territories of the detritivorous blenny, Salarias patzneri, to identify sources of dietary detritus. Samples were collected during summer and winter and analysed for fatty acid, hydrocarbon and sterol biomarkers. Sediments predominantly contained: even carbon number fatty acids, a high percentage of polyunsaturated fatty acids and a prevalence of n-heptadecane and n-pentadecane. This composition of lipids is typical of organic matter derived from recently deposited algae, or living microalgae. Similarities between sediment and filamentous algal lipids imply filamentous algae may be a major source of detritus in the sediments. Sediments did however have a higher percentage of 16:1ω7 than filamentous algae samples and this is most likely due to inputs to the sediments from diatoms and bacteria. Based on 20:5ω3 concentrations, it was estimated that diatoms accounted for 18% of the organic matter in sediments during summer and 4% in the winter, whilst 18:1ω7 concentrations suggest bacteria accounted for 10% of organic matter in both seasons. In addition, lipid biomarkers indicated that dinoflagellates, corals, cyanobacteria and zooplankton also contribute to sediments, providing a diverse range of dietary nutrients. It is this combination of inputs to sedimentary detritus that provides S. patzneri with essential dietary nutrients.

Barramundi as an indicator species for environmental monitoring in North Queensland, Australia: laboratory versus field studies.

The dose-response relationship for hepatic 7-ethoxyresorufin-O-deethylase (EROD) induction in barramundi (Lates calcarifer) was examined under controlled laboratory conditions for 15 d using farm-reared barramundi. These results were compared with EROD activity measured in barramundi collected from two rivers catchments (impacted and nonimpacted) in northern Queensland, Australia. Barramundi were dosed by intraperitoneal injection with a known cytochrome P4501A (CYP1A) inducer, beta-naphthoflavone (beta-NF), at 5, 10, and 50 mg kg(-1) using two controls: A vehicle control (corn-oil injected) and an experimental control (no injection). The EROD induction occurred within 4 h in the 5, 10, and 50 mg beta-NF kg(-1) exposures, reaching mean maximum activities of 88.6 (+/-51.9), 85.5 (+/-91.7), and 149.1 (+/-106.4) pmol min(-1) mg protein(-1), respectively. Mean EROD activities remained low in the corn-oil controls (2.1+/-1.8 pmol min(-1) mg protein(-1)) and experimental controls (5.3+/-4.4 pmol min(-1) mg protein(-1)) throughout the study. Barramundi demonstrated a rapid response curve, which was dose dependent (50 > 10 > 5 mg beta-NF kg(-1)) and decreased progressively over time from induction. Measurement of total cytochrome P450 content (nmol mg protein(-1)) was not dose dependent. The EROD activities from field-collected barramundi from the Johnstone River (impacted) and Olive River (nonimpacted) suggest exposure to low-level contaminants in the Johnstone River fish only. With more controlled laboratory and field studies, barramundi have the potential to become a major indicator species in assessing exposure to environmental contaminants in coastal areas throughout northern Queensland, Australia.