Kerkil Choi

Multiframe image estimation for coded aperture snapshot spectral imagers

A coded aperture snapshot spectral imager (CASSI) estimates the three-dimensional spatiospectral data cube from a snapshot two-dimensional coded projection, assuming that the scene is spatially and spectrally sparse. For less spectrally sparse scenes, we show that the use of multiple nondegenerate snapshots can make data cube recovery less ill-posed, yielding improved spatial and spectral reconstruction fidelity. Additionally, data acquisition can be easily scaled to meet the time/resolution requirements of the scene with little modification or extension of the original CASSI hardware. A multiframe reconstruction of a 640 × 480 × 53 voxel datacube with 450-650 nm white-light illumination of a scene reveals substantial improvement in the reconstruction fidelity, with limited increase in acquisition and reconstruction time.

Video-rate compressive holographic microscopic tomography

Compressive holography enables 3D reconstruction from a single 2D holographic snapshot for objects that can be sparsely represented in some basis. The snapshot mode enables tomographic imaging of microscopic moving objects. We demonstrate video-rate tomographic image acquisition of two live water cyclopses with 5.2 μm spatial resolution and 60 μm axial resolution.

Compressive holography of diffuse objects

We propose an estimation-theoretic approach to the inference of an incoherent 3D scattering density from 2D scattered speckle field measurements. The object density is derived from the covariance of the speckle field. The inference is performed by a constrained optimization technique inspired by compressive sensing theory. Experimental results demonstrate and verify the performance of our estimates.

Identification of fluorescent beads using a coded aperture snapshot spectral imager

We apply a coded aperture snapshot spectral imager (CASSI) to fluorescence microscopy. CASSI records a two-dimensional (2D) spectrally filtered projection of a three-dimensional (3D) spectral data cube. We minimize a convex quadratic function with total variation (TV) constraints for data cube estimation from the 2D snapshot. We adapt the TV minimization algorithm for direct fluorescent bead identification from CASSI measurements by combining a priori knowledge of the spectra associated with each bead type. Our proposed method creates a 2D bead identity image. Simulated fluorescence CASSI measurements are used to evaluate the behavior of the algorithm. We also record real CASSI measurements of a ten bead type fluorescence scene and create a 2D bead identity map. A baseline image from filtered-array imaging system verifies CASSIs 2D bead identity map.

Generalized sampling using a compound-eye imaging system for multi-dimensional object acquisition.

In this paper, we propose generalized sampling approaches for measuring a multi-dimensional object using a compact compound-eye imaging system called thin observation module by bound optics (TOMBO). This paper shows the proposed system model, physical examples, and simulations to verify TOMBO imaging using generalized sampling. In the system, an object is modulated and multiplied by a weight distribution with physical coding, and the coded optical signal is integrated on to a detector array. A numerical estimation algorithm employing a sparsity constraint is used for object reconstruction.

Inline holographic coherent anti-Stokes Raman microscopy

We demonstrate a simple approach for inline holographic coherent anti-Stokes Raman scattering (CARS) microscopy, in which a layer of uniform nonlinear medium is placed in front of a specimen to be imaged. The reference wave created by four-wave mixing in the nonlinear medium can interfere with the CARS signal generated in the specimen to result in an inline hologram. We experimentally and theoretically investigate the inline CARS holography and show that it has chemical selectivity and can allow for three-dimensional imaging.

Image-based registration for synthetic aperture holography

High pixel count apertures for digital holography may be synthesized by scanning smaller aperture detector arrays. Characterization and compensation for registration errors in the detector array position and pitch and for phase instability between the reference and object field is a major challenge in scanned systems. We use a secondary sensor to monitor phase and image-based registration parameter estimators to demonstrate near diffraction-limited resolution from a 63.4 mm aperture synthesized by scanning a 5.28 mm subaperture over 144 transverse positions. We demonstrate 60 μm resolution at 2 m range.

Imaging through turbulence using compressive coherence sensing.

Previous studies have shown that the isoplanatic distortion due to turbulence and the image of a remote object may be jointly estimated from the 4D mutual intensity across an aperture. This Letter shows that decompressive inference on a 2D slice of the 4D mutual intensity, as measured by a rotational shear interferometer, is sufficient for estimation of sparse objects imaged through turbulence. The 2D slice is processed using an iterative algorithm that alternates between estimating the sparse objects and estimating the turbulence-induced phase screen. This approach may enable new systems that infer object properties through turbulence without exhaustive sampling of coherence functions.

Thin holographic camera with integrated reference distribution

Off-axis digital holography typically uses a beam splitter to combine reference and object waves at an angle matched to the sampling period of the sensor array. The beam splitter determines the thickness of the recording system. This paper describes and demonstrates a total internal reflection hologram that replaces the beam splitter and enables hologram recording over a large aperture with a thin camera.

Compressive Holographic Microscopy

We demonstrate a snapshot 3-D holographic microscopy using decompressive inference to infer a tomographic image from a Gabor hologram and to remove autocorrelation and twin-image terms.