Kilder Dantas Filgueira

Study on coinfecting vector-borne pathogens in dogs and ticks in Rio Grande do Norte, Brazil

Since dogs presenting several vector borne diseases can show none or nonspecific clinical signs depending on the phase of infection, the assessment of the particular agents involved is mandatory. The present study aimed to investigate the presence of Babesia spp., Ehrlichia spp., Anaplasma spp., Hepatozoon spp. and Leishmania spp. in blood samples and ticks, collected from two dogs from Rio Grande do Norte showing suggestive tick-borne disease by using molecular techniques. DNA of E. canis, H. canis and L. infantum were detected in blood samples and R. sanguineus ticks collected from dogs. Among all samples analyzed, two showed the presence of multiple infections with E. canis, H. canis and L. infantum chagasi. Here we highlighted the need for molecular differential diagnosis in dogs showing nonspecific clinical signs.

Morphometric Alteration of Intestinal Epithelium of Rats (Rattus norvegicus) Submitted to the Technique of Enteropexy

Background: Due to the numerous complications that enteropexy may cause in domestic animals and humans, this study aimed at investigating the microscopic level, the damage generated by this technique in mice, in order to investigate the morphometric changes caused by enteropexy technique in rats. Materials, Methods & Results: Eighteen Wistar rats were submitted to surgical technique of enteropexy. To evaluate intestinal disorders they were euthanized at 30 (Group I), 60 (group II) and 90 (group III) days after the procedure. The animals were perfused with 2.5% glutaraldehyde and 4% paraformaldehyde for the removal of intestinal fragments for analysis in conventional microscopy and scanning electron microscopy. In classical histopathology, group I presented mild lesions with necrosis of the epithelium and infiltration of mononuclear cells. After 60 days, extensive ischemic necrosis area was observed, characterized by the denudation of epithelium cells and cellular debris in the lumen surface and loss of intestinal crypts. After 90 days (group III) lesions were evident and were characterized by strong coagulative necrosis of epithelial cells and intestinal crypts. Under electron microscopy, group I showed early fusion of the adjacent intestinal walls. After 60 days there was adherence of luminal stenosis and intestinal walls. In group III loss of epithelium and substitution of necrotic tissue were identified. Discussion: In the current study it was noticed microscopically that the groups had inflammatory reactions to foreign bodies. It is known that the sutures, independent of manufacture, behave as foreign bodies that induce tissue inflammation of the recipient organism and could harm the scar repair. Among the surgical threads, the nylon type (which was chosen for the current experiment) is indicated in the approach tissues in general and bandages, including cardiovascular, ophthalmic and neurological procedures. This category wire induces minimal inflammation of the receptor tissue. In studies of the histopathological analysis of the tissue reactions produced by the wire implant or nylon thread clamp in rats, it was observed that when the local action of two implants were compared, inflammation showed qualitatively similar responses, although they had different characteristics regarding their course. Inflammation is proportional to the proliferation of fibroblasts and the presence of fibrous tissue around the suture. This quote confirms the analyzed samples, in which, in all postoperative times showed inflammatory process, although at different magnitudes. Surgical research in laboratory animals has expanded in recent decades, mainly due to better anesthetic support, the sophistication of infrastructure, material for perioperative continuous monitoring and an incessant search for species that reproduce human morbid conditions. The main focus of these studies have been improving the knowledge about the pathophysiological mechanisms of disease, undertake therapeutic trials with new drugs, studying biomarkers and evaluate new techniques to be applied prospects in man. The most common sites of occurrence of intestinal are ileocolic intussusception junction and jejunojejunal segment. The enteroplication has been used to reduce the occurrence of intussusception, promoting the adhesion of the adjacent serosa layers of intestinal segments and thereby decreasing intestinal motility, their applicability is reported in humans, dogs and cats. The enteropexy technique causes morphological changes in intestinal epithelium of rats, evidenced in conventional and electron microscopy, progress with the passing of time the procedure.

Canine brucellosis: a pratical review for the clinical veterinarian of small animals

Brucellosis is a bacterial disease, caused by the genus Brucella, which compromises several species of domestic mammals, among which the canine species stands out. One of the major sources of contamination is direct contact with expelled tissue or genital secretions of bitches that have recently aborted. Brucella infection in dogs causes several clinical manifestations. In infected pregnant bitches, it can result in embryonic death, abortion and subsequent prolonged vaginal discharge. In males, infection is Revista Brasileira de Higiene e Sanidade Animal Brazilian Journal of Hygiene and Animal Sanity ISSN: 1981-2965 I

Association of chlorambucil and prednisolone in the treatment of canine acute myeloid leukemia

Acute myeloid leukemia (AML) is a type of neoplasm that is characterized by infiltration, especially in the bone marrow and peripheral blood, by undifferentiated cells of the hematopoietic system. In general, chemotherapy in dogs with AML is ineffective and patients have an unfavorable prognosis. The objective of this study was to describe a therapeutic protocol for the control of AML in dogs. An eight-year-old poodle bitch presented the history of apathy and weight loss. Further examinations were requested. These corresponded to hemogram, serum biochemistry (alanine Revista Brasileira de Higiene e Sanidade Animal Brazilian Journal of Hygiene and Animal Sanity ISSN: 1981-2965

Myasthenia gravis canine: A differential diagnosis for infectious diseases of origin neurological

Myasthenia gravis (MG) is a neuromuscular disorder caused by a reduction in the number of functional nicotinic acetylcholine receptors in the postsynaptic membrane. Some infectious neurological diseases can mimic this situation. The study describes a case of MG in dogs, showing the conduct in diagnosis and therapy, in addition to providing the Revista Brasileira de Higiene e Sanidade Animal Brazilian Journal of Hygiene and Animal Sanity ISSN: 1981-2965

Prostate endogenous calculi in a dog

In dogs, prostatic calculi in most cases are asymptomatic and are diagnosed through routine imaging method being benign prostatic hyperplasia (BPH) is more frequent prostatic disease that affects dogs. The objective of this manuscript is to describes one case of endogenous prostatic calculi in a dog with BPH. A 18-year-old male non neutered mongrel dog with a 3-day history of decreased appetite, bloody feces and abdominal pain. Swelling occurred in rectal palpation of the prostate. Abdominal ultrasound visualized an increase of prostate and cavitations in its parenchyma. The animal died and at necropsy was viewed a bilateral and symmetrical increase of the prostate with cystic cavitation. During the section of the same, was visualized calculations were compounds by carbonate, phosphate, calcium, magnesium and ammonia, after analyzing physical chemistry. Histopathological prostatic tissue indicated BPH. Prostatic calculi in dogs are incidentally found on routine at veterinary clinics.

Mammary neoplasias in domestic female cats: possible influence of the diet on the etiology

1 Academicas de Medicina Veterinaria, Universidade Federal Rural do Semi-Arido (Mossoro-RN) geyannanunes@yahoo.com.br 2 Profa. Dra., Departamento de Ciencias Animais, Universidade Federal Rural do SemiArido (Mossoro-RN) – valeria@ufersa.edu.br 3 Medico Veterinario, Hospital Veterinario, Universidade Federal Rural do Semi-Arido (Mossoro-RN) – cisneiros@ufersa.edu.br 4 Medico Veterinario M. Sc., Hospital Veterinario, Universidade Federal Rural do SemiArido (Mossoro-RN) – kilder@ufersa.edu.br ____________________________________________________________________

HEMANGIOMA SUBCUTÂNEO ASSOCIADO A TECIDO ECTÓPICO PULMONAR EM BOVINO NEONATO.

Na especie bovina, as neoplasias cutâneas congenitas sao raras. Todavia quando observadas, pode-se citar o hemangioma, um tumor benigno vascular. Sua associacao com tecido de origem embriologica distinta, resultante de processos ectopicos, nao sao relatados comumente. Nesse sentido, o presente trabalho objetivou a descricao de um hemangioma congenito com tecido pulmonar ectopico num bezerro. Um bovino, macho, sem raca definida, com quatro horas de vida, foi atendido com o historico de neoformacao proxima ao ânus. O paciente foi submetido a exame fisico e cirurgia para a excisao de tal neoformacao. O material obtido foi encaminhado para histopatologia. O tumor perianal era de abrangencia subcutânea e possuia 25 cm de diâmetro. A superficie de corte demonstrava a existencia de um tecido pulmonar ectopico. A analise histopatologica foi compativel com hemangioma associado a material pulmonar. O conhecimento de tais lesoes torna-se importante, pois contribui com os reduzidos relatos na literatura sobre neoplasias cutâneas congenitas em bovinos, principalmente, no que diz respeito a sua apresentacao clinico-patologica. PALAVRAS-CHAVE: Hemangioma. Bovino. Tecido ectopico. Pulmao.