Muriel Magda Lustosa Pimentel

Equine chorionic gonadotrophin improved vascularization of feline ovarian tissue xenografted into immunosuppressed mice

The objective of this study was to evaluate effects of eCG on vascularization and development of feline ovarian tissue xenografted to immunosuppressed mice. Feline ovarian fragments (∼1 mm3) were transplanted under the renal capsule of 20 adult, ovariectomized, C57BL/6 SCID female mice. At 45 d after transplantation, 10 mice (controls) were euthanized and the remainder given 10 IU of eCG (and sacrificed 48 h later). Transplants were recovered immediately after death, fixed, sectioned, and stained with periodic acid-Schiff (PAS). Fragment volume (Cavallieri principle) and vascularization were assessed. Mean xenotransplant volume for control and treatment groups was 0.17 ± 0.03 and 0.37 ± 0.13 mm3, respectively (P = 0.0952); vascular volume density, 30.3 ± 11.3 and 49.1 ± 8.9% (P = 0.0281); surface density, 4.1 ± 2.4 and 6.2 ± 1.7 μm-1 (P = 0.2222); and vessel total surface, 0.63 ± 0.24 μm2 and 2.28 ± 1.05 μm2 (P = 0.0079). In conclusion, eCG significantly increased vascular volume density of xenotransplanted ovarian tissue and improved its development.

Hematological and biochemical profile of BALB/c nude and C57BL/6 SCID female mice after ovarian xenograft

Hematological and biochemical profile studies help to evaluate functional changes of animals used in experiments. The aim of this study was to determine the hematological and biochemical profile of immunosuppressed BALB/c nude and C57BL/6 SCID mice after bovine ovarian xenotransplantation. Therefore, a total of 74 female mice were divided into four groups: non-xenotransplanted animals, xenotransplanted animals, xenotransplanted animals treated with eCG and xenotransplanted animals treated with FSH + LH. After anesthesia, blood samples were collected and hematologic and biochemical values were evaluated. The results showed no significant differences (p ≤ 0.05) for hematological parameters between the control group and the treatment groups of both strains. However, considering the biochemical profile, it was observed an increase of AST concentrations (p ≤ 0.05) in both strains and a decrease of ALT concentrations (p ≤ 0.05) only in C57BL/6 SCID strain of the groups subjected to hormonal treatment compared with those non subjected. Additionally, the values of the renal enzymes, urea and creatinine, did not differ (p ≤ 0.05) between the groups. Our findings suggest that the xenotransplantation procedure as well as the hormonal dosages had no significant effect on the well-being of the animals considering the evaluated hematological and biochemical profile.

Morphometric Alteration of Intestinal Epithelium of Rats (Rattus norvegicus) Submitted to the Technique of Enteropexy

Background: Due to the numerous complications that enteropexy may cause in domestic animals and humans, this study aimed at investigating the microscopic level, the damage generated by this technique in mice, in order to investigate the morphometric changes caused by enteropexy technique in rats. Materials, Methods & Results: Eighteen Wistar rats were submitted to surgical technique of enteropexy. To evaluate intestinal disorders they were euthanized at 30 (Group I), 60 (group II) and 90 (group III) days after the procedure. The animals were perfused with 2.5% glutaraldehyde and 4% paraformaldehyde for the removal of intestinal fragments for analysis in conventional microscopy and scanning electron microscopy. In classical histopathology, group I presented mild lesions with necrosis of the epithelium and infiltration of mononuclear cells. After 60 days, extensive ischemic necrosis area was observed, characterized by the denudation of epithelium cells and cellular debris in the lumen surface and loss of intestinal crypts. After 90 days (group III) lesions were evident and were characterized by strong coagulative necrosis of epithelial cells and intestinal crypts. Under electron microscopy, group I showed early fusion of the adjacent intestinal walls. After 60 days there was adherence of luminal stenosis and intestinal walls. In group III loss of epithelium and substitution of necrotic tissue were identified. Discussion: In the current study it was noticed microscopically that the groups had inflammatory reactions to foreign bodies. It is known that the sutures, independent of manufacture, behave as foreign bodies that induce tissue inflammation of the recipient organism and could harm the scar repair. Among the surgical threads, the nylon type (which was chosen for the current experiment) is indicated in the approach tissues in general and bandages, including cardiovascular, ophthalmic and neurological procedures. This category wire induces minimal inflammation of the receptor tissue. In studies of the histopathological analysis of the tissue reactions produced by the wire implant or nylon thread clamp in rats, it was observed that when the local action of two implants were compared, inflammation showed qualitatively similar responses, although they had different characteristics regarding their course. Inflammation is proportional to the proliferation of fibroblasts and the presence of fibrous tissue around the suture. This quote confirms the analyzed samples, in which, in all postoperative times showed inflammatory process, although at different magnitudes. Surgical research in laboratory animals has expanded in recent decades, mainly due to better anesthetic support, the sophistication of infrastructure, material for perioperative continuous monitoring and an incessant search for species that reproduce human morbid conditions. The main focus of these studies have been improving the knowledge about the pathophysiological mechanisms of disease, undertake therapeutic trials with new drugs, studying biomarkers and evaluate new techniques to be applied prospects in man. The most common sites of occurrence of intestinal are ileocolic intussusception junction and jejunojejunal segment. The enteroplication has been used to reduce the occurrence of intussusception, promoting the adhesion of the adjacent serosa layers of intestinal segments and thereby decreasing intestinal motility, their applicability is reported in humans, dogs and cats. The enteropexy technique causes morphological changes in intestinal epithelium of rats, evidenced in conventional and electron microscopy, progress with the passing of time the procedure.

Association of chlorambucil and prednisolone in the treatment of canine acute myeloid leukemia

Acute myeloid leukemia (AML) is a type of neoplasm that is characterized by infiltration, especially in the bone marrow and peripheral blood, by undifferentiated cells of the hematopoietic system. In general, chemotherapy in dogs with AML is ineffective and patients have an unfavorable prognosis. The objective of this study was to describe a therapeutic protocol for the control of AML in dogs. An eight-year-old poodle bitch presented the history of apathy and weight loss. Further examinations were requested. These corresponded to hemogram, serum biochemistry (alanine Revista Brasileira de Higiene e Sanidade Animal Brazilian Journal of Hygiene and Animal Sanity ISSN: 1981-2965

Xenotransplante de tecidos ovários em animais: limitações, técnicas e inovações. Uma revisão

Xenotransplante consiste em transplantar orgaos, tecidos e/ou celulas de animais, geneticamente modificados ou nao, entre especies diferentes. O xenotransplante ovariano e utilizado como metodo de coleta e maturacao dos foliculos e seus oocitos, e para tanto e necessario ainda aprimoramento de algumas tecnicas. Este artigo de revisao tem como objetivo, abordar aspectos fisiologicos foliculares e registrar o aperfeicoamento da tecnica e de que forma os pesquisadores vem conduzindo seus estudos nos ultimos anos em xenotransplante ovariano animal.

Myasthenia gravis canine: A differential diagnosis for infectious diseases of origin neurological

Myasthenia gravis (MG) is a neuromuscular disorder caused by a reduction in the number of functional nicotinic acetylcholine receptors in the postsynaptic membrane. Some infectious neurological diseases can mimic this situation. The study describes a case of MG in dogs, showing the conduct in diagnosis and therapy, in addition to providing the Revista Brasileira de Higiene e Sanidade Animal Brazilian Journal of Hygiene and Animal Sanity ISSN: 1981-2965

Impact of pulmonary lesions in cattle for slaughter in the Municipality of Mossoró, RN

Aimed to investigate the main pulmonary changes found in cattle intended for slaughter slaughtering and industrial Refrigerator Natal, RN, from October 2013 to January 2014. Inspection was performed post mortem advocated by Regulation inspection of products of animal origin 2386 animal animals were divided into two groups: the coming Mossoro or from other states. Data were collected through records and analyzed using descriptive statistics (percentage) and frequency of variables found by chi square test. The results showed that 71 % (n = 1695) examined the lungs were considered healthy and 29 % ( n = 691) had some type of injury . Furthermore, 48.7 % (n = 337) of the convictions were of animals from Mossley and 51.3 % (n = 354) from other states . With regard to pulmonary disorders , emphysema (28.8 %) , congestion (28 %) , aspiration of blood ( 12.3 %), pneumonia (8.8%) , aspiration of rumen contents (8 %) , hyperemia were observed ( 8%), abscess (2.7%) , edema (2.4%) and pneumonia (1.0%). Both groups showed similar results when compared to each other, except for abscess (p = 0.02) and agonal aspiration of blood ( p = 0.00 ) in the animals of the region of Natal, edema (p = 0.04) and emphysema ( p = 0.03 ) for animals from other regions of the country. It was concluded that animal stunning at slaughter is important, influencing the appearance of pulmonary changes, being related to a standard of efficiency, adequacy and accuracy training on stunning and people involved in the process.

Prostate endogenous calculi in a dog

In dogs, prostatic calculi in most cases are asymptomatic and are diagnosed through routine imaging method being benign prostatic hyperplasia (BPH) is more frequent prostatic disease that affects dogs. The objective of this manuscript is to describes one case of endogenous prostatic calculi in a dog with BPH. A 18-year-old male non neutered mongrel dog with a 3-day history of decreased appetite, bloody feces and abdominal pain. Swelling occurred in rectal palpation of the prostate. Abdominal ultrasound visualized an increase of prostate and cavitations in its parenchyma. The animal died and at necropsy was viewed a bilateral and symmetrical increase of the prostate with cystic cavitation. During the section of the same, was visualized calculations were compounds by carbonate, phosphate, calcium, magnesium and ammonia, after analyzing physical chemistry. Histopathological prostatic tissue indicated BPH. Prostatic calculi in dogs are incidentally found on routine at veterinary clinics.

Avaliação do perfil eritrocitário de jumentos nordestinos (Equus asinus) suplementados com hematínico a base de vitaminas, sais minerais e glicose

Este trabalho avaliou as alteracoes causadas pela administracao diaria de um hematinico a base de vitaminas do complexo B e K, cobre, cobalto, zinco, ferro e glicose no perfil eritrocitario de asininos da raca Nordestina. Para tanto, foram utilizados oito asininos, que durante 35 dias permaneceram estabulados recebendo alimentacao balanceada e agua a vontade, alem do suplemento em questao. Os parâmetros analisados foram hematocrito, numero de hemacias, concentracao de hemoglobina, volume corpuscular medio, concentracao de hemoglobina corpuscular media, como tambem a observacao das celulas vermelhas, atraves de esfregaco sanguineo. O hematocrito, hemacias, concentracao de hemoglobina, VCM e CHCM demonstraram aumento significativo apos a utilizacao do composto, porem nao foram observadas alteracoes nas analises dos esfregacos. O suplemento, ao termino do experimento, foi capaz de melhorar o perfil eritrocitario dos animais.