Kyosuke Tsuda

Sterol compositions in some green algae and brown algae

Abstract Compositions of the sterol in Chlorophyta and Phaeophyta were established quantitatively. Sterol content in Chlorophyta is more similar to higher plant, and it contains large amount of cholesterol. In Phaeophyta, fucosterol is the main sterol and small amounts of cholesterol, 24-methylene-cholesterol and saringosterol were identified.

Gas chromatographic analysis of oxidation products of adrenal corticosteroids

Abstract Gas chromatographic separations of periodic acid and sodium bismuthate oxidation products of corticosteroids, including cortexolone, cortisone, cortisol, prednisolone, deoxycorticosterone, dehydrocorticosterone, and corticosterone, were investigated using SE-30, QF-1, and XE-60 phases.

Transformation of steroids by Pseudomonas chlororaphis (studies on steroids and microorganisms part VI)

Abstract The transformation of steroids by Pseudomonas chlororaphis was examined in detail. The following facts were found: 1) This strain is capable of the degradation of the side chain of a number of C21-steroids. One of the products of the degradation of 17α,-21-dihydroxypregna-1,4-dien-3,20-dione is 1-dehydrotestololactone. 2) This strain displays a specific 17β-hydroxysteroid dehydrogenase activity.

Individual and total quantitative determination of prednisolone and hydrocortisone.

プレドニソロン(PRと略記)中,ハイドロコーチゾン(HCと略記)が混在する場合の全ステロイド量の定量,ならびに両ステロイドの分離定量をTPTZ法でおこなった.呈色反応の溶媒として使用するエタノールは,含水量が少ないほどよく,また反応温度は25℃が最適とわかった.比重0.802のエタノール,25℃,40～45分間の反応で485mμに安定な一定の吸光度を示し,HCとPRの検量線が全く重なることを見出した.この結果にもとづき, TPTZ法によるHCとPR混合試料の全ステロイド量,およびペーパークロマトグラフィーによる分離後,各スポット抽出液より,HCとPRの分離定量が可能となった.

Untersuchungen uber Steroide. XIV. Reduktion von Reichsteins Substanz S mittels Zink in Essigsaure.

Beobachtungen bei der Reduktion von Reichsteins Substanz S (I) mittels Zink werden beschrieben. Hierbei konnen Cortexon (II), 17-Isocortexon (III) und 17-Isoprogesteron (IV) entstehen. Diese Ergebnisse werden diskutiert.