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Dive into the research topics where Laura Riva is active.

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Featured researches published by Laura Riva.


Journal of Lipid Research | 2013

Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages

Laurent L'Homme; Nathalie Esser; Laura Riva; André Scheen; Nicolas Paquot; Jacques Piette; Sylvie Legrand-Poels

The NLRP3 inflammasome is involved in many obesity-associated diseases, such as type 2 diabetes, atherosclerosis, and gouty arthritis, through its ability to induce interleukin (IL)-1β release. The molecular link between obesity and inflammasome activation is still unclear, but free fatty acids have been proposed as one triggering event. Here we reported opposite effects of saturated fatty acids (SFAs) compared with unsaturated fatty acids (UFAs) on NLRP3 inflammasome in human monocytes/macrophages. Palmitate and stearate, both SFAs, triggered IL-1β secretion in a caspase-1/ASC/NLRP3-dependent pathway. Unlike SFAs, the UFAs oleate and linoleate did not lead to IL-1β secretion. In addition, they totally prevented the IL-1β release induced by SFAs and, with less efficiency, by a broad range of NLRP3 inducers, including nigericin, alum, and monosodium urate. UFAs did not affect the transcriptional effect of SFAs, suggesting a specific effect on the NLRP3 activation. These results provide a new anti-inflammatory mechanism of UFAs by preventing the activation of the NLRP3 inflammasome and, therefore, IL-1β processing. By this way, UFAs might play a protective role in NLRP3-associated diseases.


Biology | 2014

Hepatitis C Virus Life Cycle and Lipid Metabolism

Costin-Ioan Popescu; Laura Riva; Ovidiu Vlaicu; Rayan Farhat; Yves Rouillé; Jean Dubuisson

Hepatitis C Virus (HCV) infects over 150 million people worldwide. In most cases HCV infection becomes chronic, causing liver disease ranging from fibrosis to cirrhosis and hepatocellular carcinoma. HCV affects the cholesterol homeostasis and at the molecular level, every step of the virus life cycle is intimately connected to lipid metabolism. In this review, we present an update on the lipids and apolipoproteins that are involved in the HCV infectious cycle steps: entry, replication and assembly. Moreover, the result of the assembly process is a lipoviroparticle, which represents a peculiarity of hepatitis C virion. This review illustrates an example of an intricate virus-host interaction governed by lipid metabolism.


Journal of Virology | 2013

ORF9p phosphorylation by ORF47p is crucial for the formation and egress of Varicella-­‐ Zoster Virus viral particles.

Laura Riva; Marc Thiry; Sébastien Bontems; Aline Joris; Jacques Piette; Marielle Lebrun; Catherine Sadzot-Delvaux

ABSTRACT The role of the tegument during the herpesvirus lytic cycle is still not clearly established, particularly at the late phase of infection, when the newly produced viral particles need to be fully assembled before being released from the infected cell. The varicella-zoster virus (VZV) protein coded by open reading frame (ORF) 9 (ORF9p) is an essential tegument protein, and, even though its mRNA is the most expressed during the productive infection, little is known about its functions. Using a GalK positive/negative selection technique, we modified a bacterial artificial chromosome (BAC) containing the complete VZV genome to create viruses expressing mutant versions of ORF9p. We showed that ORF9p is hyperphosphorylated during the infection, especially through its interaction with the viral Ser/Thr kinase ORF47p; we identified a consensus site within ORF9p recognized by ORF47p and demonstrated its importance for ORF9p phosphorylation. Strikingly, an ultrastructural analysis revealed that the mutation of this consensus site (glutamate 85 to arginine) strongly affects viral assembly and release, reproducing the ORF47 kinase-dead VZV phenotype. It also slightly diminishes the infectivity toward immature dendritic cells. Taken together, our results identify ORF9p as a new viral substrate of ORF47p and suggest a determinant role of this phosphorylation for viral infectivity, especially during the process of viral particle formation and egress.


Virology | 2014

Varicella-zoster virus induces the formation of dynamic nuclear capsid aggregates.

Marielle Lebrun; Nicolas Thelen; Marc Thiry; Laura Riva; Isabelle Ote; Claude Condé; Patricia Vandevenne; Emmanuel Di Valentin; Sébastien Bontems; Catherine Sadzot-Delvaux

The first step of herpesviruses virion assembly occurs in the nucleus. However, the exact site where nucleocapsids are assembled, where the genome and the inner tegument are acquired, remains controversial. We created a recombinant VZV expressing ORF23 (homologous to HSV-1 VP26) fused to the eGFP and dually fluorescent viruses with a tegument protein additionally fused to a red tag (ORF9, ORF21 and ORF22 corresponding to HSV-1 UL49, UL37 and UL36). We identified nuclear dense structures containing the major capsid protein, the scaffold protein and maturing protease, as well as ORF21 and ORF22. Correlative microscopy demonstrated that the structures correspond to capsid aggregates and time-lapse video imaging showed that they appear prior to the accumulation of cytoplasmic capsids, presumably undergoing the secondary egress, and are highly dynamic. Our observations suggest that these structures might represent a nuclear area important for capsid assembly and/or maturation before the budding at the inner nuclear membrane.


Journal of Virology | 2015

Deletion of the ORF9p acidic cluster impairs the nuclear egress of varicella-zoster virus capsids.

Laura Riva; Marc Thiry; Marielle Lebrun; Laurent L'Homme; Jacques Piette; Catherine Sadzot-Delvaux

ABSTRACT The protein encoded by ORF9 is essential for varicella-zoster virus (VZV) replication. Previous studies documented its presence in the trans-Golgi network and its involvement in secondary envelopment. In this work, we deleted the ORF9p acidic cluster, destroying its interaction with ORF47p, and this resulted in a nuclear accumulation of both proteins. This phenotype results in an accumulation of primary enveloped capsids in the perinuclear space, reflecting a capsid de-envelopment defect.


Journal of Virology | 2018

Identification of Piperazinylbenzenesulfonamides as New Inhibitors of Claudin-1 Trafficking and Hepatitis C Virus Entry

Laura Riva; Ok-Ryul Song; Jannick Prentoe; François Helle; Laurent L'Homme; Charles-Henry Gattolliat; Alexandre Vandeputte; Lucie Fénéant; Sandrine Belouzard; Thomas Baumert; Tarik Asselah; Jens Bukh; Priscille Brodin; Laurence Cocquerel; Yves Rouillé; Jean Dubuisson


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Journal of Virology | 2018

Varicella-Zoster Virus ORF9p Binding to Cellular Adaptor Protein Complex 1 Is Important for Viral Infectivity

Marielle Lebrun; Julien Lambert; Laura Riva; Nicolas Thelen; Xavier Rambout; Caroline Blondeau; Marc Thiry; Robert Snoeck; Jean-Claude Twizere; Franck Dequiedt; Graciela Andrei; Catherine Sadzot-Delvaux


Archive | 2017

The role of the tegument protein ORF9p and its phosphorylation status in VZV nuclear egress

Marielle Lebrun; Caroline Blondeau; Laura Riva; Julien Lambert; Marc Thiry; Bernard Sadzot


Archive | 2017

CHARACTERIZATION OF THE COMPLEX FORMED BY THE VARICELLA ZOSTER VIRUS ORF9P AND THE CELLULAR ADAPTIN PROTEIN-1 AND ITS ROLE IN THE SECONDARY EGRESS

Julien Lambert; Marielle Lebrun; Judit Sanchez-Gil; Laura Riva; Catherine Sadzot

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Caroline Blondeau

Institut national de la recherche agronomique

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