Lavínia Schwantes

Multidrug resistance gene expression during the murine ontogeny

Multidrug resistance (MDR) was described initially for tumor cells which become resistant not only to the specific drug to which they are submitted, but also to a large range of unrelated drugs. The expression of mdr genes, responsible for the phenotype, and their product P glycoprotein (Pgp), is currently under intensive study due to their ample distribution in different organisms and their possible physiological roles which include protection against xenobiotics. In mice, three mdr isoforms expressed in some normal tissues are known. In this work, we analyzed by RT-PCR the expression of mdr1, mdr2 and mdr3 in several organs of BALB/c and C57BL/6 mice during ontogeny. A considerable variation in mdr expression among individuals of the same strain, as well as among different organs in individuals of the same age group and among different age groups, was detected. We also observed a strong tendency for the expression of a greater number of active isoforms in old mice. The large expression range of the mdr isoforms point to an important role as a natural defense system.

Expression of mdr isoforms in mice during estrous cycle and under hormone stimulation

The multidrug resistance (MDR) phenotype is associated with the expression of P-glycoprotein (Pgp), coded by the multigenic mdr family. Mice present the isoforms mdr1 and mdr3, which are responsible for multidrug resistance, and mdr2, that is involved in the transport of phospholipids. mdr1 expression has more recently been associated also with the secretion of steroid hormones. This work presents an RT-PCR analysis of the expression of mdr isoforms, in several organs of mice during different phases of the estrous cycle. Additionally, females were ovariectomized, submitted to different hormone treatments, and their uterus was analyzed for the expression of mdr isoforms. The results show that in the adrenal gland and ovaries mdr1 is the main isoform during proestrus, and that progesterone or a combination of progesterone and estrogen induce the expression of all mdr isoforms in the uterus of ovariectomized females. We suggest that the functions of mdr1 and mdr3 are overlapping, that mdr3 may be the more efficient isoform in the detoxification function, and that mdr1 may be more closely related to the secretion of steroid hormones.

FAZER O DESCONHECIDO SER DESCOBERTO NOVOS TALENTOS DA REDE PÚBLICA (RNEC/NT)

The modern discourse of science is still established as the episteme of our times. It is present in many research and extension projects like the one of the Education and Science National Network, linking many universities across the country. In this study, we sought analyzing the science discourse of southern groups from the Network. Weused some foucaultian tools of discourse analysis to investigate the interviews with researchers and observers of these groups. We noticed that many respondents link their speeches to the modern aspect of science due to different contexts. However, some cracks and flexibilizations have been happening in the discourses of some groups, producing a loosening of the recurrent discourse of science.

Discurso sobre a Ciência na emergência histórica da "Rede Nacional de Educação e Ciência"

Diferentes projetos para promocao do acesso a ciencia por meio da educacao tem sido desenvolvidos, como a Rede Nacional de Educacao e Ciencia, que objetiva a melhoria do acesso a ciencia e do seu ensino por meio de cursos para professores e alunos da rede publica de ensino. Objetivamos estabelecer o discurso inicial de ciencia dessa Rede. A metodologia empregou conceitos e ferramentas da analise do discurso foucaultiana. Elencamos tres enunciados que caracterizam o discurso inicial de ciencia na emergencia dessa Rede: fazer ciencia envolve um caminho para geracao de produtos “novos” publicaveis; formacao do cientista na diade inatismo e empirismo; formacao de cientistas pela inclusao social. Esses enunciados podem ser entendidos como condicoes de possibilidade para emergencia da Rede e sao potentes modos de desenvolver e produzir a ciencia na atualidade.

HACER EL DESCONOCIDO SER DESCUBIERTO: ACTUALIZACION EM LOS MODOS DE ENUNCIAR LA CIENCIA EM LOS GRUPOS DE LA REGIÓN SUR DE LA RED NACIONAL DE EDUCACIÓN Y CIENCIA - NUEVOS TALENTOS DE LA RED PÚBLICA (RNEC/NT)

The modern discourse of science is still established as the episteme of our times. It is present in many research and extension projects like the one of the Education and Science National Network, linking many universities across the country. In this study, we sought analyzing the science discourse of southern groups from the Network. Weused some foucaultian tools of discourse analysis to investigate the interviews with researchers and observers of these groups. We noticed that many respondents link their speeches to the modern aspect of science due to different contexts. However, some cracks and flexibilizations have been happening in the discourses of some groups, producing a loosening of the recurrent discourse of science.

MAKING THE UNKNOWN KNOWN: AN UPDATE ON THE WAYS OF ENUNCIATING SCIENCE IN THE SOUTHERN GROUPS OF THE EDUCATION AND SCIENCE NATIONAL NETWORK- NEW TALENTS OF THE PUBLIC SYSTEM (RNEC/NT)

The modern discourse of science is still established as the episteme of our times. It is present in many research and extension projects like the one of the Education and Science National Network, linking many universities across the country. In this study, we sought analyzing the science discourse of southern groups from the Network. Weused some foucaultian tools of discourse analysis to investigate the interviews with researchers and observers of these groups. We noticed that many respondents link their speeches to the modern aspect of science due to different contexts. However, some cracks and flexibilizations have been happening in the discourses of some groups, producing a loosening of the recurrent discourse of science.