Leandro José Ramos

Adaptation of the Micronucleus Technique in Allium Cepa, For Mutagenicity Analysis of the Jamari River Valley, Western Amazon, Brazil

This study aimed to make technique adjustment of micronuclei in Allium cepa, recommended by Fiskejo [1], by replacing methanol and Giemsa by triarilmetano the 0,1%, xantenos the 0,1% and tiazinas the 0,1%. We obtained good results with the replacement, as its efficiency in stain nucleic acids, obtaining a good visualization of cells, mitotic index, micronuclei, anaphase and telophase bridges, and this methodology is indicated in future examinations of mutagenicity in the river Vale do Jamari.

New method for detection of mutagenicity in oral mucosa the through of micronucleus test

Background: The micronucleus assay using a tissue rapidly dividing, such as human oral mucosa, allows for assessment of damage of genetic material, without the need for a stage of ex vivo cell replication. Methods: The present study describes a new protocol for the detection of MN in oral mucosa, replacing methanol and giemsa by triarilmetano, xanthene and thiazine. Results: The blades were well visible with ungrouped cells, having a good view of the cell boundaries. Conclusions: Good results were obtained with the substitution of methanol and giemsa by triarilmetano, thiazine and xanthene, because of its efficiency in blush nucleic acid, getting a good visualization of cell nuclei and micronucleus, and this methodology it is indicated for mutagenicity analysis of the oral mucosa.

First report of Triatoma sordida Stål, 1859 (Hemiptera, Reduviidae, Triatominae) in the State of Acre and Brazilian Western Amazon

INTRODUCTION The occurrence of Triatoma sordida in the Brazilian Western Amazon is reported for the first time. METHODS Triatoma sordida specimens were collected from a Gallus gallus nest in a peridomiciliary area of Senador Guiomard municipality in the state of Acre. RESULTS The number of triatomine species in Acre increased from six to seven with this first report of T. sordida in the Brazilian Western Amazon. CONCLUSIONS The occurrence of T. sordida in Acre is of concern since it is among the most captured triatomines in peridomiciliary environments in Brazil, and carries a high potential for vector transmission.

First report of Rhodnius neglectus (Hemiptera, Reduviidae, Triatominae) from the State of Acre, Brazil, and the Brazilian Western Amazon Region

INTRODUCTION This communication reports the occurrence of Rhodnius neglectus in the State of Acre, Brazil. METHODS An adult male R. neglectus specimen was collected from the Catuaba Experimental Reserve, which is located in the municipality of Senador Guiomard. RESULTS This increases the number of triatomine species reported from the State of Acre to eight and is also the first report of R. neglectus from the Brazilian Western Amazon Region. CONCLUSIONS The occurrence of R. neglectus is alarming because even though the species is wild, it can invade and colonize human dwellings and peridomiciles, thereby increasing Chagas disease transmission.

First report of Panstrongylus megistus (Hemiptera, Reduviidae, Triatominae) in the State of Acre and Rondônia, Amazon, Brazil

INTRODUCTION This article reports, for the first time, the occurrence of Panstrongylus megistus in the Brazilian Western Amazon. METHODS Specimens of P. megistus were collected in the cities of Rio Branco, Acre and Extrema, Rondônia. RESULTS The number of triatomine species in the State of Acre increased from eight to nine and in Rondônia from seven to eight. This was also the first report of P. megistus in the Brazilian Western Amazon. CONCLUSION The occurrence of P. megistus in the Western Amazon evidences an epidemiological alert, since it is an important vector of T. cruzi.

Rhodnius stali: new vector infected by Trypanosoma rangeli (Kinetoplastida, Trypanosomatidae)

INTRODUCTION Rhodnius stali infection by Trypanosoma rangeli is reported in this study for the first time. METHODS The triatomines were collected from the campus of the Federal University of Acre in Rio Branco, Acre, Brazil. The identification of T. rangeli was confirmed by multiplex polymerase chain reaction. RESULTS The examinations of two specimens revealed R. stali infection by the epimastigote forms of T. rangeli. CONCLUSIONS The encounter of R. stali infected by T. rangeli generates an alert for the state of Acre, since the simultaneous presence with Trypanosoma cruzi can make the differential diagnosis of Chagas disease difficult.