Lijuan Ren

Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai

Fusarium head blight (FHB) is a destructive disease of wheat worldwide. FHB resistance genes from Sumai 3 and its derivatives such as Ning 7840 have been well characterized through molecular mapping. In this study, resistance genes in Wangshuibai, a Chinese landrace with high and stable FHB resistance, were analyzed through molecular mapping. A population of 104 F2-derived F7 recombinant inbred lines (RILs) was developed from the cross between resistant landrace Wangshuibai and susceptible variety Alondra‘s’. A total of 32 informative amplified fragment length polymorphism (AFLP) primer pairs (EcoRI/MseI) amplified 410 AFLP markers segregating among the RILs. Among them, 250 markers were mapped in 23 linkage groups covering a genetic distance of 2,430 cM. In addition, 90 simple sequence repeat (SSR) markers were integrated into the AFLP map. Fifteen markers associated with three quantitative trait loci (QTL) for FHB resistance (P < 0.01) were located on two chromosomes. One QTL was mapped on 1B and two others were mapped on 3B. One QTL on 3BS showed a major effect and explained up to 23.8% of the phenotypic variation for type II FHB resistance.

Inheritance of stem strength and its correlations with culm morphological traits in wheat (Triticum aestivum L.)

Yao, J., Ma, H., Zhang, P., Ren, L., Yang, X., Yao, G., Zhang, P. and Zhou, M. 2011. Inheritance of stem strength and its correlations with culm morphological traits in wheat ( Triticum æestivum L.). Can. J. Plant Sci. 91: 1065-1070. The genetic effect of stem strength and its correlation with culm morphological traits were investigated in a 7×7 diallel cross of wheat involving seven parents (Ningmai 8, Ningmai 9, Yangmai 5, Yangmai 9, Yangmai 11, Sumai 3, and Wangshuibai) during the crop season of 2009-2010. Significant differences were observed among genotypes for stem strength. The estimates of general combining ability (GCA) pointed out that the best general combiners for stem strength were Ningmai 8 and Yangmai 9. The additive-dominance model was adequate for stem strength, and it was controlled by the over dominance type of gene action. Ningmai 8, followed by Yangmai 5, possessed maximum recessive genes, whereas Wangshuibai had maximum dominant genes. Stem strength could be controlled by three genes with low narrow sense heritability. A statistical analysis showed that stem strength is highly significantly correlated with nine culm morphological traits except for diameter of the first basal internode.

Enhancement of Resistance to Fusarium Head Blight and Sharp Eyespot in Gastrodianin Transgenic Wheat: Enhancement of Resistance to Fusarium Head Blight and Sharp Eyespot in Gastrodianin Transgenic Wheat

天麻抗真菌蛋白Gastrodianin在体外可以抑制多种病原真菌的生长。为检验转 Gastrodianin 基因小麦对真菌病害的抗性，采用基因枪法将由 ubiquitin 启动子驱动的 Gastrodianin 基因导入小麦品种扬麦158和Alondra, 获得14株纯合的转基因株系。外源基因的PCR检测、染色体荧光原位杂交、半定量RT-PCR分析结果表明，外源 Gastrodianin 基因在转基因小麦T 5 代植株中已经纯合并有不同水平的表达；赤霉病和纹枯病抗性鉴定结果显示， Gastrodianin 基因的表达能抑制病原菌在转基因植株中生长，从而减轻病原菌引起的病症发展，且两种病害的减轻程度与 Gastrodianin 基因的表达水平正相关。