Lisiane Dorneles de Lima

Avaliação das exigências nutricionais de pequenos ruminantes pelos sistemas de alimentação recentemente publicados

Um experimento foi conduzido com o objetivo de verificar os efeitos de diferentes relacoes fluor:fosforo na alimentacao sobre o desempenho de frangos de corte. Foram utilizados 1.000 pintos de corte de 1 dia distribuidos em um delineamento inteiramente casualizado, com quatro tratamentos e cinco repeticoes de 50 aves por boxe. Os tratamentos consistiram de quatro fontes de fosforo com relacoes fluor:fosforo de 1:40, 1:60, 1:80 e 1:100. O experimento foi dividido em tres fases experimentais: 1 a 21, 22 a 42 e 43 a 49 dias de idade. Em cada fase, avaliou-se o consumo de racao, o ganho de peso e a conversao alimentar. Ao final do experimento, foram abatidas duas aves de cada repeticao para coleta da tibia e de musculos do peito para analise das concentracoes.

Progresso científico em pequenos ruminantes na primeira década do século XXI

Worldwide interest in small ruminants has increased in recent years in both, the productive and scientific aspects. In Brazil, the same trend has been observed, however, among all small ruminants, goats and sheep can be highlighted. Thus, this review aimed to discuss scientific progress in goat and sheep in the last decade. Data were collected in different databases, such as Institute for Scientific Information (ISI), Scientific Electronic Library Online - SciELO, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - CAPES, Instituto Brasileiro de Geografia e Estatistica - IBGE and Food and Agriculture Organization of the United Nations - FAO. In the last decade, goat and sheep have received particular attention from researchers, which can be proved by the number of publications involving these species, which increased 41% worldwide, and nationwide increase was even more expressive, 219%. Methodological aspects applied in researches have also improved, emphasizing multidisciplinary approach. Much of the scientific progress was due to increasing financial support through thematic calls demanded by society and by the productive chain of meat and milk. In these calls is noticeable the concern with product quality, competitiveness and sustainability.

Mineral Metabolism in Singleton and Twin-pregnant Dairy Goats

During pregnancy, the maternal body undergoes significant physiological changes. The present study assessed the changes on calcium (Ca), phosphorus (P), magnesium (Mg), sodium (Na) and potassium (K) metabolism in singleton and twin-pregnant dairy goats. The 42 goats used (49.5 kg±7.6 body weight [BW]) were assigned at random to treatments that were factorially arranged to account for 2 breeds (Oberhasli and Saanen), 2 pregnancy types (singleton and twin) and 3 gestation periods (80, 110, and 140 days). Digestibility trials were performed at 80, 110, and 140 days of gestation. Mineral retention during pregnancy was determined in the maternal body, femur, uterus, mammary gland, fetus and fetal fluid. Blood samples were taken during pregnancy before and after a meal, and Ca, P, Mg, Na, K ions and alkaline phosphatase activity determined in serum. Bone mineral density was determined in the right femur. Statistical analyses were performed using the SAS MIXED procedure. Dry matter intake decreased linearly up to 140 days of gestation. Maternal BW gain, and Ca, P, and Mg retention (g/kg) decreased linearly with the advance of gestation days. Macromineral retention in maternal body (g/kg) was greater in Oberhasli than Saanen goats, and their fetuses had higher Ca, P, and Mg deposition (mg/g). Mineral retention (mg/g) increased in fetuses according to pregnancy development, with no differences between singleton and twin pregnancy. In the mammary gland, the retention of all minerals (g) increased with the days of pregnancy. In conclusion, related to Ca, P, and Mg metabolism can be divided into two stages. Up to 80 days of gestation, was characterized by the preparation of the maternal body reserves for future mineral demands. From 80 days of gestation onward, was characterized by the transfer of maternal body reserves for fetal development and colostrum production. Na and K supply was provided by adjustments in endogenous excretion and an increase in intestinal absorption. Finally, mineral metabolism was specific to each genotype and, except for Na, was not affected by the number of fetuses.

Changes in maternal body composition and metabolism of dairy goats during pregnancy

The objective of this study was to evaluate the mobilization of nutrients in goats of different gestation types and pregnancy stages. Forty-four Saanen and Oberhasli goats were studied. The goats of each breed and gestation type (single or twin) were slaughtered at different gestational ages (80, 110, and 140 days of pregnancy), forming a completely randomized design in a 2 × 3 × 2 factorial arrangement (two breeds, three gestational ages, and two types of pregnancy). The slaughter procedure involved separating the empty body, mammary glands, uterus with membranes and fetal fluid, and fetus(es). For the females slaughtered at 140 days of pregnancy, blood was collected to analyze metabolites and hormones every 15 days during gestation. The dry matter (DM) intake was lower in goats with twin pregnancies. The relative daily retention rate of the nutrients in the body was positive at 100 days of pregnancy but became negative at 140 days (-0.18±0.25 g DM kg-1 of maternal body d-1) and did not differ with breed or number of fetuses. Fetal growth in twin pregnancies was 66% higher than in single pregnancies. The highest levels of s-hydroxybutyrate and non-esterified fatty acids were observed beginning at 100 days of gestation. Serum total protein and albumin levels decreased after 125 days of gestation. Serum urea levels were reduced after 80 days of gestation. Plasma 17s-estradiol levels increased with the advance of pregnancy, and IGF-1 was highest between 60 and 80 days of gestation. The maternal metabolism throughout pregnancy does not vary with the type of pregnancy, and pregnant goats need greater nutritional intake during the final third of the gestational period regardless of the breed or type of pregnancy.

Ultrasonographic measurements of kidney fat thickness and Longissimus muscle area in predicting body composition of pregnant goats

Non-invasive methods for estimating body composition to monitor nutritional status in goats have been less studied. Therefore, this study estimated the body composition and fat depots of pregnant goats by using indirect measurements, such as bodyweight (BW) and ultrasound measurements of the Longissimus muscle area (LMA) and kidney fat thickness (KFT). Forty-three pregnant goats were slaughtered at 50, 80, 110 and 140 days of gestation to determine the gross energy and fat content in the body, as well as to obtain the mass of renal fat, omental fat and non-carcass fat. After the slaughter, the mass of fat depots was recorded and the whole body of the each animal was ground to take samples for determining gross energy (using a bomb calorimeter) and fat (Soxhlet extraction with ether). Animals were weighed, and ultrasonographic measurements were taken at the time of slaughter. The LMA and KFT measurements were taken between the 12th and 13th rib and after the 13th rib space, respectively, and these measurements were used to estimate total gross energy, body fat content and the renal fat, omental fat and non-carcass fat mass. Multiple regressions were performed using Proc mixed of SAS. No significant correlation was observed between gross energy and body fat composition, the number of fetuses or the days of gestation. LMA and BW were positively related to body fat and gross energy contents. KFT and BW showed a positive correlation with renal fat depots, omental fat content and non-carcass fat mass. The estimated models revealed that the total body fat increased from 15.6% to 20.8% of bodyweight from 50 to 140 days of gestation. This report is the first study using kidney fat thickness as a tool to predict fat depots in goats. LMA, BW and KFT measurements will enable the development of models to aid in the monitoring of the nutritional status of goats during pregnancy.

Do carcasses of lambs finished on commercial production systems meet market standards

This study aimed to characterize a commercial lamb finishing system using animals of undefined breed from production to slaughter by analyzing performance, carcass traits, yield of commercial cuts, and the quality and meat acceptance of different slaughter groups, as to evaluate whether this system provides the market with a standardized product. The lots were not homogeneous for yield of commercial cuts and performance and morphometric traits evaluated in vivo. The groups were heterogeneous to 75% of the 13 carcass traits evaluated, among them, hot and cold carcass weights, hot and cold carcass yields, carcass grade finishing and biological yield. There was also no uniformity for the proportion of non-carcass components, morphometry of carcass, visual appraisals, and loin traits. On the other hand, homogeneity was achieved in physico-chemical and sensory traits, except for hardness and proportion of saturated, monounsaturated and polyunsaturated fatty acids. We conclude that the commercial finishing system with the use of undefined crossbred lambs does not produce carcass and cuts standardized to the market.

Energy and protein requirements for maintenance of dairy goats during pregnancy and their efficiencies of use

It has been suggested that maintenance requirements are similar among animals of different physiological stages; however, important physiological changes occur in the maternal body during pregnancy. Therefore, the aim of this study was to determine the energy and protein requirements for the maintenance of pregnant dairy goats and to estimate their efficiency of energy and protein utilization for maintenance and pregnancy. We used 66 multiparous pregnant goats having 49.0 ± 1.59 kg initial BW (around the third or fourth parturition) arranged in a randomized block design with a 3 × 3 factorial scheme including slaughter at different days of pregnancy (DOP; 80, 110, and 140 d) and feed restriction (0, 20, and 40% feed restriction). The comparative slaughter technique was used to estimate energy and protein maintenance requirements. Goats slaughtered at 140 DOP were subjected to digestibility trials at around 80, 110, and 140 DOP to estimate diet metabolizability and N balance (NBAL). Metabolizability decreased with feed restriction and was 63.3 ± 2.16, 55.7 ± 2.35, and 58.2 ± 2.30% at 0, 20, and 40% of feed restriction, respectively ( < 0.01). There was no effect of DOP on NE or the requirements of ME for maintenance (ME), which were 197 and 315 kJ/kg empty body weight (EBW), respectively, and the efficiency of ME utilization for maintenance (k) was 0.63. Similarly, DOP did not affect thedaily net protein requirements for maintenance (NP) estimated using the comparative slaughter technique (1.38 ± 0.512 g/kg EBW; = 0.003) or the NP estimated using NBAL (2.49 ± 0.594 g/kg EBW; < 0.01). The MP requirement for maintenance (MP) estimated using the comparative slaughter technique was not affected by DOP and was 3.22 g MP/kg EBW ( < 0.01). The efficiency of MP utilization for maintenance (k) was 0.43. The efficiency of ME utilization for pregnancy (k) increased with the progress of pregnancy and was 0.058, 0.10, and 0.19 at 80, 110, and 140 DOP, respectively. Similarly, the efficiency of MP utilization for pregnancy (k) increased with DOP and was 0.12, 0.21, and 0.43 at 80, 110, and 140 DOP, respectively. There was no evidence that pregnancy affected NE, ME, NP, and MP or k and k, which were also unaffected by DOP. However, k and k increased with pregnancy progress as a response to the physiological changes that pregnant females are subjected to.

Mineral metabolism of pregnant goats under feed restriction

We examined the effects of feed restriction on calcium, phosphorus, magnesium, sodium and potassium metabolism in Oberhasli and Saanen goats during gestation. The 63 goats were distributed into groups that were divided into three levels of feed restriction (0%, 20% and 40% restriction) and slaughtered at different pregnancy stages (80, 110 and 140 days of gestation), in a randomised block design with a 2 × 3 × 3 factorial arrangement. The mineral balance was determined at ~80, 110 and 140 days of gestation. The serum levels of minerals and alkaline phosphatase activity were determined during pregnancy. Mineral retention in the maternal body, femur, empty uterus, mammary gland, fetus and fetal fluid was also determined during gestation. Bone mineral density was measured in the femur. Mixed models with days of gestation, levels of feed restriction, breed and their interactions as fixed effects and blocks as random effect were used for data analysis. In response to the reduction in feed intake, the maternal body uses its mineral reserves to maintain gestation. Physiological adjustments of the goats subjected to 20% feed restriction avoided a decrease in fetal mineral deposition. More severe feed restriction, however, compromised concentrations of phosphorus, sodium and potassium in the fetus, which were the main minerals used by the maternal body, whereas calcium and magnesium deposition in fetuses remained unaffected. At 40% feed restriction, the retention of all minerals in the body decreased, and the fetal dry mass was on average also less than those fetuses from goats without feed restriction. The fetal deposition of phosphorus, sodium and potassium was also lower during 40% restriction.