Marcelo Diniz dos Santos

Aspecto radiológico e macroscópico de matriz óssea mineralizada heteróloga fragmentada e polimetilmetacrilato autoclavados em falha óssea de tíbia de coelho

A segmental defect of 6mm diameter was performed in the medial metaphyseal region of the tibia of 12 rabbits. For the bone defect reconstruction there was implanted a combination of micro and macro fragments of heterologous fragmented cortical bone matrix preserved in glycerin (98%) and polymethylmethacrylate, both autoclaved. Radiological and macroscopic evaluation was performed at 30, 60, 90 and 120 days. Adhesion in relation to time of the micro and macro composites to the recipient bed was observed in 100% of the cases. This showed that this implant is biologically biocompatible, since it promoted bone defects repair, with no signs of infection, migration and/or rejection. In this way, this implant can be classified as one more option of substitute to fill large bone defects.

Avaliação morfológica e por imagem radiográfica da matriz óssea mineralizada heteróloga fragmentada e metilmetacrilato, preservados em glicerina para reparação de falhas ósseas em tíbias de coelhos

The traumas that result in bone fractures, especially comminuted, have high importance in veterinary and human surgical routine. A 6mm of segmental defect was performed at the medial metaphyseal region of the left tibia of 12 rabbits and an association of fragmented heterologue mineralized bone matrix and methylmethacrylate conserved in glycerin (98%) was used as a graft to fill the bone defect. To evaluate the procedure morphological and radiological exams were performed after 30, 60, 90 and 120 days. There was gradual integration of the bone graft in the receptor bed in 100% of the cases showing that the material is biologically compatible as it promotes bone defect reparation without signs of infection, migration and/or rejection and can be considered one more option to be used as a substitute to fill bone defects.

Concentração sérica de testosterona em touros Zebu

The objectives of this study were to evaluate the effects of two levels of concentrate and lipids in the diets on the testosterone serum concentration of Zebu bulls, as well as the daily-nightly recurrent behavior of the referred hormone. Sixteen Zebu bulls were allotted to four treatments during 90 days of experimental period: T1 (high concentrate and high lipid), T2 (high concentrate and low lipid), T3 (low concentrate and high lipid), and T4 (low concentrate and low lipid). The diets were formulated to be isoproteics and the forage to concentrate ratio was 45:55% (T1 and T2) and 80:20 % (T3 and T4). Blood samples were collected from 15 to 15 days at fixed intervals of four hours, during 24 hours, to determine the testosterone serum concentration. The intake of dry matter, ether extract, and crude protein was greater for the animals fed diets with high level of concentrate and smaller for the animals fed diets with low concentrate levels. The neutral detergent fiber (NDF) and ether extract intake were greater in the animals fed diets with low level of concentrate and high level of lipid, respectively. Serum testosterone concentration were not affected by the different concentrate and lipid levels in the diets and ranged from .1 to 9.0 ng/mL, with average of 1.24 ng/mL. There was a difference in the serum testosterone concentration in relation to the observed collection time, being the collection times of 4:30 p.m. and 00:30 a.m. presenting the highest serum testosterone concentration in relation to the 4:30 and 12:30 h, and it did not differ from the others collection times, however, it was not characterized as a surge.

Morphology and Morphometry of Seminiferous Tubules in Crioulo Horses

Abstract This study aimed to assess the biometrics of the testes and the morphology of the seminiferous tubules of Crioulo horses. We studied 10 sexually mature stallions (3–6 years of age). After orchiectomy, testes were perfused with Karnovskys solution and then embedded in glycol methacrylate. Testis sections (4 &mgr;m) were cut and stained with toluidine blue and a solution of 1% sodium borate. The histological images were digitized, and the morphometric analysis was performed using ImageJ software. The average weight of the stallions was 377.5 kg, and the average weight of both testicles was 162.9 g. The percentage of testicular parenchyma occupied by the seminiferous tubules and the intertubular tissue was 77.97% ± 6.34% and 22.03% ± 6.34%, respectively. The average tubular diameter was 205.00 ± 36.91 &mgr;m, whereas the average height of the seminiferous epithelium was 70.56 ± 2.82 &mgr;m. Average tubular length per testicle and average tubular length per gram of testicle were 4,085.10 ± 1,170.68 m and 26.09 ± 10.63 m/g, respectively. The characteristics of the eight stages of the seminiferous epithelium cycle were similar to those reported in other horse breeds. We conclude that the morphometry of the seminiferous tubules of Crioulo horse resembles what has been reported in other horse breeds. The volumetric proportion of the seminiferous tubules and the Leydig cells of the Crioulo horse is one of the highest ever reported for stallions. HighlightsThis is the first article describing the morphology of spermatogenesis Crioulo horses.The volumetric proportion of the seminiferous tubules of the Crioulo horse is one of the highest ever reported for stallions.The proportion of the Leydig cells of the Crioulo horse is one of the highest ever reported for stallions.

Bicarbonato de sódio como preventivo da acidose metabólica em ovinos submetidos à acidose ruminal experimental

The aim of this study was to evaluate the preventive effect of sodium bicarbonate on systemic acidosis due to ruminal acidosis, which was induced by ingestion of concentrate after prolonged fasting. Fourteen sheep were divided into three experimental groups: control group (Cg), with four sheep, submitted to fasting without development of ruminal acidosis; no-treated group (NTg), with five sheep with rumen acidosis without preventive treatment; and treated group (Tg), with five sheep with rumen acidosis and preventively treated with sodium bicarbonate. Assessments of ruminal pH and arterial hemogasometry were performed for 48 hours after ingestion of the concentrate. There was a reduction in the ruminal pH in all groups, whereas the Cg showed a reduction only after 24 hours. A reduction in the arterial pH, bicarbonate and base excess in all groups was also noted, indicating systemic metabolic acidosis, but the NTg presented the greatest alteration. It is concluded that sodium bicarbonate prevents systemic metabolic acidosis, reducing its severity in sheep subjected to ruminal acidosis.

Testicular Histomorphometric Evaluation of Zebu Bull Breeds

ABSTRACT The objective of this study was to evaluate the quantitative histology and testicular biometrics in zebu bulls of different breeds. Testicular fragments of Nelore (n=10), Polled Nelore (n=6), Gir (n=5), Guzerat (n=5) and Tabapua bulls (n=5) were used. The fragments were perfusion-fixed in Karnovsky solution, embedded in glycol methacrylate and stained with toluidine blue-1% sodium borate. The Nelore animals had a higher tubular volumetric proportion (85.2%) and greater height of the seminiferous epithelium (73.2 µm) than the Gir, Guzerat and Tabapua breeds. The Nelore animals also had a higher volumetric proportion of Leydig cells (5.2%) than the Guzerat and Tabapua breeds. There was no significant difference for any of these parameters between the Nelore and Polled Nelore breeds. The gonadosomatic index, seminiferous tubule diameter, cross-sectional area of the seminiferous tubule and tubule length (total length and length per gram of testicular parenchyma) did not vary among the breeds studied. The morphometric parameters evaluated suggested that the genetic selection applied to the Nelore and Polled Nelore breeds improved the efficiency of spermatogenesis in these breeders. Key words: cattle, testis, volumetric proportion, seminiferous tubule

Corporal and testicular biometry in wild boar from birth to 12 months of age

Objetivou-se, com esta pesquisa, investigar o desenvolvimento testicular e corporal de javalis (Sus scrofa scrofa), do nascimento aos 12 meses de idade, avaliando-se, em cada faixa etaria, o peso corporal, o desenvolvimento biometrico dos testiculos e o indice gonadossomatico (IGS). Utilizaram-se 52 javalis machos, provenientes de um criatorio comercial, devidamente regulamentado pelo IBAMA. Os animais foram pesados e divididos em 13 grupos experimentais; os testiculos foram coletados por orquiectomia unilateral e, em seguida, pesados em balanca analitica e mensurados comprimento, largura e espessura. Os valores de peso corporal e as medidas testiculares foram crescentes, apos o nascimento, sendo sua variacao mais acelerada no primeiro mes e aos nove meses. Inicialmente, o padrao de crescimento testicular, do nascimento aos nove meses de idade, acompanhou o crescimento corporal e o indice gonadossomatico variou de 0,07 a 0,09%. Em seguida, entre nove e 11 meses, o valor de crescimento testicular foi superior ao de crescimento corporal e o indice gonadossomatico variou de 0,09 a 0,16%. Por fim, apos os 11 meses de idade, os valores crescimento corporal e dos testiculos voltaram a se comportar de forma semelhante. Conclui-se que o desenvolvimento do peso corporal, a biometria testicular e o IGS tiveram crescimento mais acelerado aos nove meses de idade.

Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls

espanolRESUMEN El objetivo de este estudio fue evaluar la ecotextura testicular de los toros Nelore y correlacionarlo con los parametros de espermatogenesis. Para este proposito, 41 toros de edades de 3-5 anos y con pesos corporales en el intervalo de 510,2 ± 21,61 kg fueron seleccionados para el estudio. Los toros se sometieron a ecografia, utilizando un dispositivo portatil junto con un transductor lineal a una frecuencia de 7,5 MHz. Inmediatamente despues, los animales fueron castrados, y el tejido testicular se trato con Karnovsky fijador. Los fragmentos testiculares se incluyeron en metacrilato de glicol y se tineron con una solucion de borato de sodio-azul de toluidina al 1%. La intensidad media de pixeles en el parenquima testicular se determino para secciones de superficie de 1.600 mm2. Los El area entre los dos testiculos derecho e izquierdo dieron lugar a la intensidad media de los pixeles de 105,22 ± 13,58, 107,95 ± 12,82 y 106,59 ± 11,43, respectivamente. El coeficiente de eficiencia de la mitosis espermatogonial fue 19,74; Indice meiotico fue 2,63, y el rendimiento global del espermatogenesis alcanzo el 52,0. No se observo correlacion (P>0,05) entre ecotextura testicular y cualquiera de los parametros morfometricos medidos. Estos resultados sugieren que ecotextura testicular no es un metodo viable para el analisis indirecto de la poblacion celular en el epitelio seminifero, el rendimiento de la espermatogenesis intrinseco, o el indice de celulas de Sertoli en toros Nelore. EnglishABSTRACT The objective of this study was to evaluate the testicular echotexture of Nelore bulls and to correlate it with spermatogenic parameters. Forty one bulls aged 3-5 years, with body weight of 510.2 ± 21.61 kg were selected for the study. The animals were subjected to ultrasonography, using a portable device coupled with a linear transducer at a frequency of 7.5 MHz. Immediately afterwards they were castrated and the testicular tissue was treated with Karnovsky fixative. Next, the fragments were embedded in glycol methacrylate and stained with toluidine blue-1% sodium borate solution. The average pixel intensity in the testicular parenchyma was determined for 1,600-mm2 surface sections. The right and left testicles and the area between the two testicles resulted in average pixel intensities of 105.22 ± 13.58, 107.95 ± 12.82, and 106.59 ± 11.43, respectively. The efficiency coefficient of spermatogonial mitosis was 19.74; meiotic index was 2.63, and overall yield of spermatogenesis was 52.0. No correlation (P>0.05) was observed between testicular echotexture and any of the measured morphometric parameters. These results suggest that testicular echotexture was not a viable method for the indirect analysis of cellular population in the seminiferous epithelium, intrinsic spermatogenic performance, or the Sertoli cell index in Nelore bulls.

Bovine pulmonary lesions found in post-mortem inspection in slaughterhouse in state Paraná

Polyana Carolina Marino, Gercio Luiz Bonesi, Luiz Carlos Negri Filho, Daniella Furlan, Débora Nayara Augusto, Alexey Leon Gomel Bogado, Rogério Anderson Marcasso, Luiz Cesar da Silva, Marcelo Diniz dos Santos, Werner Okano 1 Docente. Departamento de Clínica Médica e Cirúrgica de Grandes Animais. UNINGA, Maringá, PR. pollyveterinaria@gmail.com 2 Docente. Medicina Veterinária. UNOPAR. Arapongas, PR. gercio.bonesi@agricultura.gov.br; rogeriomarcasso@gmail.com 3 Mestrando do Programa de Pós Graduação em Saúde de Produção de Ruminantes. PROSUP/CAPES. UNOPAR. Arapongas, PR. luiz.negri@hotmail.com; furlandanie@hotmail.com; debora_augusto@outlook.com 4 Docentes do Programa de Pós Graduação em Saúde de Produção de Ruminantes. UNOPAR. Arapongas, PR. alexey_leon@hotmail.com; silvaluizcesar@gmail.com; werner.okano@unopar.br 5 Docente do Programa de Pós Graduação em Biociência Animal. UNIC. Cuiabá, MT. marcelo.diniz@kroton.com.br __________________________________________________________________________________

Evaluation of hematological and biochemical parameters of bulls fed diets with different levels of free gossypol

The use of whole cottonseed feed to ruminants has attracted great interest particularly since it is an important supplemental energy and protein source for pastures. In Brazil, several studies have already evaluated the negative effects of gossypol contained in cottonseed on animal reproduction. However, the evaluation of its effects on hematological and biochemical parameters are incipient. Consequently, to evaluate the effects of the ingestion of free gossypol on the hematological and biochemical profiles of bulls animals, samples from 30 Nellore bulls, averaging 30 ± 6 months of age and live weight of 383 kg were collected at the beginning (day 0) and end of the experiment (day 95 day). All bulls were randomly distributed into six treatments, each having five replicates: T0, 0 gram (g) of free gossypol/bull/day (cottonseed free); T1, 1 g of free gossypol/bull/day; T2, 2 g of free gossypol/bull/day; T3, 3 g of free gossypol/bull/day; T4, 4 g of free gossypol/bull/day and T5, 5 grams of free gossypol/bull/day. The results demonstrated that the hematological and biochemical values were within the physiological limits for cattle. However, some treatments revealed significant statistical differences (P<0.05) for the packed cell volume, red blood cells, gamma-glutamyl transferase (GGT), and urea during the experiment. Clinical manifestations suggestive of intoxication by gossypol were not observed.