Luciene Pinheiro Vianna
Universidade Federal do Rio Grande do Sul
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Publication
Featured researches published by Luciene Pinheiro Vianna.
International Journal of Developmental Neuroscience | 2007
Francieli M. Stefanello; Fernando Kreutz; Emilene B. S. Scherer; Ana Carolina Breier; Luciene Pinheiro Vianna; Vera Maria Treis Trindade; Angela Terezinha de Souza Wyse
Neurological dysfunction is observed in patients with severe hypermethioninemia, whose physiopathology is still poorly understood. In the current study we investigated the effect of chronic administration of methionine on the content and species of gangliosides and phospholipids, as well as on the concentration of cholesterol in rat cerebral cortex. Wistar rats received subcutaneous injections of methionine (1.34–2.68 μmol/g of body weight), twice a day, from the 6th to the 28th day of age and controls received saline. Animals were killed 12 h after the last injection. Results showed that methionine administration significantly decreased the total content of lipids in cerebral cortex of rats. We also observed that this amino acid significantly reduced the absolute quantity of the major brain gangliosides (GM1, GD1a, GD1b and GT1b) and phospholipids (sphingomyelin, phosphatidylcholine and phosphatidylethanolamine). We also showed that Na+,K+‐ATPase activity and TBARS were changed in cerebral cortex of rats subjected to hypermethioninemia. If confirmed in human beings, these data could suggest that the alteration in lipid composition, Na+,K+‐ATPase activity and TBARS caused by methionine might contribute to the neurophysiopathology observed in hypermethioninemic patients.
Metabolic Brain Disease | 2005
Siomara da Cruz Monteiro; Francieli M. Stefanello; Luciene Pinheiro Vianna; Cristiane Matté; Jaqueline Barp; Adriane Belló-Klein; Vera Maria Treis Trindade; Angela Terezinha de Souza Wyse
In the present work we investigated the effect of ovariectomy on acetylcholinesterase (AChE) activity and ganglioside content in cerebral cortex of female rats. We also studied the activity of butyrylcholinesterase (BuChE) in serum of these animals. Adult Wistar rats were divided into three groups: (1) naive females (control), (2) sham-operated females and (3) castrated females (ovariectomy). Thirty days after ovariectomy, rats were sacrificed by decapitation without anaesthesia. Blood was collected and the serum used for BuChE determination. Cerebral cortex was homogenized to determine AChE activity and extracted with chlorophorm:methanol for ganglioside evaluation. Results showed that rats subjected to ovariectomy presented a significant increase of AChE activity, but did not change the content and the profile of gangliosides in cerebral cortex when compared to sham or naive rats. BuChE activity was decreased in serum of rats ovariectomized. Our findings suggest that the alteration in the activity of brain AChE, as well as serum BuChE activity caused by ovariectomy may contribute to the impaired cognition and/or other neurological dysfunction found in post-menopausal women.
International Journal of Developmental Neuroscience | 2008
Luciene Pinheiro Vianna; Daniela Delwing; Andréa Gisiane Kurek; Ana Carolina Breier; Fernando Kreutz; Fabria Chiarani; Francieli M. Stefanello; Angela Terezinha de Souza Wyse; Vera Maria Treis Trindade
In the present work we investigated the effects of chronic proline administration on ganglioside, cholesterol and phospholipid total contents, as well as on ganglioside profile in cerebral cortex, hippocampus, hypothalamus and cerebellum of rats. We also evaluated the ganglioside content and profile in detergent‐soluble and resistant microdomains isolated from synaptic membranes obtained from cerebral cortex. Proline solution (hyperprolinemic) or saline (control) were subcutaneously administered to rats from 6th to 28th post‐natal day, according to body weight. Twelve hours after the last injection, the animals were sacrificed by decapitation without anaesthesia. Brain structures were homogenized with chloroform:methanol for lipid extraction. Synaptic membranes were obtained by differential centrifugation and detergent‐soluble and resistant microdomains were isolated by cold Triton X‐100 treatment. Results showed that rats subjected to chronic proline treatment presented a significant increase of ganglioside content in cortex and hippocampus, while this membrane lipid content was not altered in hypothalamus and cerebellum. Besides, phospholipid and cholesterol contents were not modified in all structures studied. On the other hand, ganglioside content decreased in detergent‐soluble and resistant microdomains isolated from synaptic membrane obtained from hyperprolinemic cortex. Although ganglioside profiles were apparently not modified, the individual absolute quantities were altered in cortex and hippocampus total lipid extract and membrane microdomains. Our findings suggest that chronic proline treatment affects in a distinct manner different cerebral regions concerning the lipid composition of the cell membranes, reflecting on its distribution in the cortex membrane microdomains. Among these phenomena consequences, distinct modulations in synaptic transmission may be suggested which might contribute to the impairment in cognition and/or other neurological dysfunctions found in hyperprolinemia type II patients.
Brazilian Journal of Pharmaceutical Sciences | 2010
Maria Viviane Gomes Muller; André Torres Petry; Luciene Pinheiro Vianna; Ana Carolina Breier; Kristiane Michelin-Tirelli; Ricardo Flores Pires; Vera Maria Treis Trindade; Janice Carneiro Coelho
Gaucher disease is a sphingolipidosis that leads to an accumulation of glucosylceramide. The objective of this study was to develop a methodology, based on the extraction, purification and quantification of glucosylceramide from blood plasma, for use in clinical research laboratories. Comparison of the glucosylceramide content in plasma from Gaucher disease patients, submitted to enzyme replacement therapy or otherwise, against that from normal individuals was also carried out. The glucosylceramide, separated from other glycosphingolipids by high performance thin layer chromatography (HPTLC) was chemically developed (CuSO4 / H3PO4) and the respective band confirmed by immunostaining (human anti-glucosylceramide antibody / peroxidase-conjugated secondary antibody). Chromatogram quantification by densitometry demonstrated that the glucosylceramide content in Gaucher disease patients was seventeen times higher than that in normal individuals, and seven times higher than that in patients on enzyme replacement therapy. The results obtained indicate that the methodology established can be used in complementary diagnosis and for treatment monitoring of Gaucher disease patients.
Revista de Ensino de Bioquímica | 2011
Vera Maria Treis Trindade; Bruno Pastoriza; Luciene Pinheiro Vianna; Christianne Gazzana Salbego; José Claudio Del Pino
Archive | 2011
Vera Maria Treis Trindade; Bruno Pastoriza; Luciene Pinheiro Vianna; Christianne Gazzana Salbego; José Claudio Del Pino
Archive | 2007
Fernando Kreutz; Melissa Camassola; Ana Carolina Breier; Alessandra Nejar Bruno; Luciene Pinheiro Vianna; Pedro Cesar Chagastelles; Fátima Theresinha Costa Rodrigues Guma; Nance Beyer Nardi
Archive | 2007
Ana Carolina Breier; Luciene Pinheiro Vianna; Daniela Delwing de Lima; Fernando Kreutz; Fabria Chiarani; Angela Terezinha de Souza Wyse
Archive | 2006
Tatiana Dourado Hoffmann; Luciene Pinheiro Vianna; Daniela Delwing de Lima; Fabria Chiarani; Angela Terezinha de Souza Wyse
Archive | 2006
Fernando Kreutz; Francieli Moro Stefanello; Tatiana Dourado Hoffmann; Ana Carolina Breier; Emilene B. S. Scherer; Luciene Pinheiro Vianna; Angela Terezinha de Souza Wyse
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Angela Terezinha de Souza Wyse
Universidade Federal do Rio Grande do Sul
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