Luiz Alberto do Lago

Efeito da condição corporal ao parto sobre alguns parâmetros do metabolismo energético, produção de leite e incidência de doenças no pós-parto de vacas leiteiras

One hundred and eighteen Holstein cows were used to evaluate the effects of body condition score at parturition on milk production and fat content, blood beta-hydroxybutirate (BHBA), glucose and aspartate-aminotransferase (AST) and disease occurence in early lactation. According to their body condition score (BCS) at calving, 1 (thin) at 5 (fat), the cows were alloted in two groups: I) composed by BCS ³ 4,0; and II) composed by BCS £ 3,5. The BCS were 4.2 and 3.2 for the group I and II, respectively. The BCS at calving did not affect milk production, milk fat, plasma glucose and AST and disease occurence in early lactation. However, the group I had higher (P<0.0084) BHBA and lost more (P=0.01) weight. When the analysis was performed weekly the BHBA for the group I was higher until three weeks post-partum, showing a peak at first and second week for both groups. The group I also had lower levels of plasma glucose in the first and second weeks, showing a intense negative energy balance in this period.

Clinical, hematological and biochemical parameters of dairy cows experimentally infected with Vaccinia virus

Vaccinia virus (VACV) is the etiological agent of bovine vaccinia (BV), an important zoonosis that affects dairy cattle. There are many aspects of the disease that remain unknown, and aiming to answer some of these questions, the clinical, hematological, and biochemical parameters of VACV experimentally infected cows were evaluated. In the first part of the study, lactating cows were infected with VACV-GP2 strain. In the second part, animals previously infected with VACV-GP2 were divided into two treatment groups: Group 1, immunosuppressed cows; and Group 2, re-infected cows. In this study, BV could be experimentally reproduced, with similar lesions as observed in natural infections. Moreover, a short incubation period and local lymphadenopathy were also observed. VACV could be detected by PCR and isolated from scabs taken from teat lesions of all inoculated and re-inoculated animals. Lymphocytosis and neutrophilia were observed in all animals from the first part of the experiment, and lymphopenia and relative neutrophilia were observed in the immunosuppressed animals. Detection of viral DNA in oral mucosa lesions suggests that viral reactivation might occur in immunosuppressed animals. Moreover, clinical disease with teat lesions may occur in previously VACV-infected cows under the experimental conditions of the present study.

Hemograma, bioquímica sérica e histologia da biópsia hepática de bovinos após administração de polpa cítrica

Hemogram and serum biochemistry (aspartate aminotransferase, alkaline phosphatase and gamma glutamiltransferase, total protein, urea, creatinine, calcium and phosphorus) were performed weekly in five crossbreed bovine after consumption of a diet containing citrus pulp pellets (40%), for 43 days. Percutaneous hepatic biopsy and histologic evaluation were performed in each animal before and after consumption of the citrus pulp diet. Hemogram, the enzymes aspartate aminotransferase and gamma glutamiltransferase, urea and creatinine had normal levels at the end of the experiment. No histologic lesions were observed in liver samples before or after citrus pulp consumption. However, there was an increase of serum phosphorus and reduction of serum calcium (p<0.05), without hypercalcemia, after consumption of citrus pulp diet. There was also an increase in serum alkaline phosphatase (p<0.05), probably induced by bone isoenzyme.

Mandibular lymphosarcoma in a goat

* Estimates were based on p30 band intensity from 10-fold CSF concentrates relative to intensities of purified bovine 14-3-3 standards (0, 10, 30 and 50 ng/ml) present on each PVDF immunoblot membrane. Detection sensitivity by antigen dilution was 0.2 ng/ml. The >50 ng/ml p30 bands were exceptionally intense. Category assignments were made independently by three experienced laboratory personnel similarly to Zerr and others (1998) t Normal cattle CSF specimens were obtained from UK/Irish farms that are historically free of BSE. Immunohistochemistry and histopathology were not performed t Symptomology and histopathology confirmed by field veterinarians and scientists of MAFF and DAFF

Quadro clínico do envenenamento crotálico experimental em bovinos (Crotalus durissus terrificus - crotamina positivo)

With the aim of describing the clinical picture of experimental crotalic poisoning in cattle, five crossbred female, from two to three years old, were inoculated with type crotalic positive crotamine poison. The dosage was 0.03 mg/kg of body weight. The animals showed chronologically, the following clinical picture: at second hour of evaluation apathy, low head, deep lethargy and discreet swelling at the inoculation place; at sixth hour, local oedema had disappeared and myoclonia was observed in great muscle masses; at tenth hour, there was decreasing of muscular tonus, as well as superficial reflexes, motor incoordination appearing and esternal recumbency; at fourteenth hour it was observed pedal movement and loss of deep sensibility; at sixteenth hour, hind limbs flaccid paralysis and after twenty hours after poisoning it was observed dyspnea, salivation and death of all animals. Rectal temperature did not alter until the twentieth hour, however, from this time it decreased constantly until the death. The respiratory frequency kept within normal range for cattle, despite dyspnea observed since twentieth hour. Clinical course duration was 28 ± 9 hours and, even though these clinical alterations are common to other diseases, it can be judged that the speed and order of appearing are determinant factors in differential diagnosis.

Análise biomecânica ex vivo de um modelo de haste intramedular de polipropileno para osteossíntese em úmeros de bezerros

A total of 12 pairs of humeri from male calves 15 to 30 days old were used. The left humeri remained unchanged and the right ones were fractured in the diaphysis between proximal and middle thirds. The fracture was fixed with a polypropylene intramedullary nail interlocked with two steel bone screws crossed along each segment. Six pairs of bones were subjected to a compression test and the others to a flexural test using a universal testing machine. In the compression tests, the mean rupture loads were 738,3N and 473,3N, and in the flexural tests they were 322,4N and 117,9N for the intact and fractured bones respectively. Fractured bones fixed with the proposed model were able to resist 66.4% of the load during compression and 36.6% during bending when in comparison to intact bones. Fractured humeri treated with polypropylene intramedullary nail showed limited resistance compared to intact ones.

Perfil bioquímico sorológico de bovinos inoculados experimentalmente com veneno crotálico iodado livre e iodado incorporado em liposomas

It was studied the activity of the Crotalus venom detoxified encapsulated in lipossomes or non-encapsulated by the method of controlled iodination on the biochemical profile of bovines. Eleven 18-months-old female bovines, weighting 160kg in average were used in this study. Groups of five animals were inoculated intramuscularly with a dose of 0,03mg/kg with either the detoxified non-encapsulated venom or the detoxified encapsulated venom. One control animal received the same dose of non detoxified venom by the same route. No significant differences were observed between the study groups for the parameters of total protein, urea and creatinine. Differences were observed for creatine phosfokinase at times TO and T12 for the group treated with the iodinated venom encapsulated in liposomes. The detoxification process abolished the activity of the venom in the encapsulated or nonencapsulated form on the biochemical parameters and renal activity of bovine.

Análise de pesticidas, aflatoxinas e análise de macro e microminerais de polpa cítrica peletizada comercial

Pesticides, aflatoxins, and nutritional analyses of commercial citrus pulp pellets in Brazil were performed in samples from Sao Paulo State. They were stored during 86 days and were processed for detection of toxic agents. Residual pesticides (organochlorinated, organophosphorus, pyrethoids, carbamates, chloroalquil tio fungicides, triazols, and the miscellaneous compound propargit) and aflatoxins (B1, B2, G1 and G2) were assayed at 0, 43 and 86 days of storage. It was not detected residual pesticide and aflatoxins in any sample. The analysis indicated reduced levels of phosphorous and high values of calcium. Assessment of micro minerals indicated the presence of aluminum, barium, titanium, cooper, manganese, thorium, vanadium, zinc, lanthanum, samarium, cobalt, antimony, arsenic, and scandium.