M.A. Bustamante

Co-composting of distillery wastes with animal manures: carbon and nitrogen transformations in the evaluation of compost stability.

The aim of this work was to study the viability of recycling the solid wastes generated by the winery and distillery industry by means of co-composting with animal manures, as well as to evaluate the quality of the composts obtained. Two piles, using exhausted grape marc and cattle manure or poultry manure, respectively (at ratios, on a fresh weight basis, of 70:30), were composted by the Rutgers static pile composting system. Throughout the composting process, a number of parameters were monitored, such as pH, electrical conductivity, organic matter, water-soluble carbon, water-soluble polyphenols, different forms of nitrogen (organic nitrogen, ammonium and nitrate) and humification indices (humification ratio, humification index, percentage of humic acid-like C, polymerisation ratio and cation exchange capacity), as well as the germination index. Organic matter losses followed first-order kinetics equation in both piles, the highest organic matter mineralisation rate being observed with exhausted grape marc and cow manure. On the other hand, the mixture with the lowest C/N ratio, using exhausted grape marc and poultry manure, showed the highest initial ammonium contents, probably due to the higher and more labile N content of poultry manure. The increase in the cation exchange capacity revealed the organic matter humification during composting. In contrast, other humification parameters, such as the humification ratio and the humification index, did not show the expected evolution and, thus, could not be used to assess compost maturity. Composting produced a degradation of the phytotoxic compounds, such as polyphenols, to give composts without a phytotoxic character. Therefore, composting can be considered as an efficient treatment to recycle this type of wastes, due to composts presented a stable and humified organic matter and without phytotoxic effects, which makes them suitable for their agronomic use.

Spent mushroom substrates as component of growing media for germination and growth of horticultural plants.

This research work was conducted in order to investigate the possibility of using spent mushroom substrate (SMS) in the production of horticultural seedlings replacing part of the peat in the growing media. Three vegetable species with different salt sensitivities, the less sensitive being tomato (Lycopersicon esculentum var. Muchamiel), the moderately salt-sensitive being courgette (Cucurbita pepo L. var. Afrodite F1) and the most salt-sensitive being pepper (Capsicum annum L. var. Lamuyo F1) were grown in 12 media containing SMS of two types of mushroom (Agaricus bisporus (SMS-AB) and Pleurotus ostreatus (SMS-PO)) or a mixture of both 50% (v/v) (SMS-50), as well as peat in various ratios. The proportions of each residue in the mixtures elaborated with peat were 25%, 50%, 75% and 100% v/v residue. A substrate of 100% peat was used as control. The experiment was arranged in a completely-randomised design with two replicates per treatment under greenhouse conditions. Prior to sowing, some physical, physico-chemical and chemical properties of the growing media were determined and seed germination and fresh weight of seedling were also measured. In most of the cases, the addition of SMS to the growing media produced an increase in the pH values, salt contents, macro and micronutrient concentrations and a decrease in the water holding capacity contents in comparison to peat, whereas great differences were found in the air capacity values between SMS-based substrates and peat. Up to 75% SMS can be used in mixtures with peat for seed germination of the plant species studied. Regarding the most suitable SMS-based substrates for plant growth, any substrate could be used for tomato seedling production. However, all SMS-AB-based substrates and the media containing low dose of SMS-PO and SMS-50 were adequate for growth of courgette and pepper.

Utilisation of manure composts by high-value crops: safety and environmental challenges.

The intensification in livestock production has increased the need of efficient treatments of waste streams especially to preserve as much as possible, the nutrients into the soil-plant system. Composting is a cheap, efficient and sustainable treatment for solid wastes that is always included in any manure treatment scenario. In this paper, an overview about the environmental and safety challenges of composting of manures is made considering the compost quality requirements established by the main demanding sectors. Co-composting and additive strategies are presented as feasible options for the improvement of compost quality. For quality evaluation of manure compost, the use of both classical and innovative instrumental techniques could increase our knowledge about added properties in compost, especially those related to organic matter stability.

Influences of winery–distillery waste compost stability and soil type on soil carbon dynamics in amended soils

The application of organic materials to replenish soil organic matter and improve soil structure and fertility has become a common agronomic practice. This research deals with the effects of soil amendment with winery and distillery waste composts on organic carbon (C) mineralisation in two arable soils. A sandy-loam and clay-loam soil were treated and incubated with a number organic materials obtained from the co-composting of different proportions of grape stalk, grape marc, exhausted grape marc and vinasse, with sewage sludge or animal manure. Moreover, the effect of compost stability on C mineralisation dynamics was studied by applying organic materials from different stages of the composting process. The results obtained showed that the addition of exogenous organic matter stimulated microbial growth, enhanced soil respiration and increased water-extractable C contents in both soils, particularly in the days immediately following amendment. The initial composition of the different organic materials used, especially for the mature samples, and the texture of the receiving soil did not influence significantly the C mineralisation final values, with around 11-20% of the added organic C being mineralised over the first 140 days. However, the contribution of organic amendment to the labile organic C pool, maximum rates of soil respiration, as well as the extent of initial disturbance of the soil microbiota were all found to be related to the degree of organic matter stability. Moreover, irrespective of the type and stability of the organic amendment, the mineralogical composition of the receiving soil was found to significantly influence its resilience in such systems.

Evolution of the pathogen content during co-composting of winery and distillery wastes

The aim of this study was to monitor some microbial indicators and pathogen contents (sulphite reducers clostridia, total enterobacteriaceae, total coliforms, faecal coliforms (Escherichia coli), enterococci, Staphylococcus aureus and Salmonella spp.) throughout the co-composting of wastes from the winery and distillery industry with other organic residues, as well as the effect of the composting system used. Seven different piles using mixtures of winery-distillery wastes with other organic materials were prepared. P1 and P2 were made using grape stalk (GS), grape marc (GM), exhausted grape marc (EGM) and sewage sludge (SS), whereas in P3 and P4 were also used exhausted grape marc with cow manure (CW) and poultry manure (PM), respectively, using the Rutgers system. Additionally, P2 was watered with vinasse (V). The rest of piles (P5, P6 and P7) were prepared with grape marc, exhausted grape marc, cow manure and poultry manure, using the turning system. The effectiveness of the composting process to reduce the pathogen content was higher in the static aerated piles than in those elaborated with the turning. The relatively high temperatures (50-60 degrees C) reached in some of the piles produced a notable decrease in some microbial groups, such as total and faecal coliforms (E. coli), but the characteristics of the raw materials used notably influenced the pathogen contents of the end-product.

Study of the composting process of winery and distillery wastes using multivariate techniques

Physico-chemical, chemical and biological parameters were studied throughout the composting process of four winery and distillery composts and the data set of compost characteristics was analysed using multivariate techniques: factorial analysis (FA) and linear discriminant analysis (LDA), in order to classify the different parameters studied and thus, to establish those that better describe the composting process of this type of wastes. Through factorial analysis (FA) of the parameters studied throughout the composting process, four components that explained 85.6% of the variability were established. The parameters associated to compost maturity, agronomic character, water-soluble fraction and ammonia and temperature increment were grouped in the components F1, F2, F3 and F4, respectively, which can reduce the number of determinations needed to ascertain the maturity and quality of the composts. In addition, the linear discriminant analysis on the factorial components makes possible to classify the four composts with a percentage of success around 95%.

The potential of near infrared reflectance spectroscopy (NIRS) for the estimation of agroindustrial compost quality.

Composting is an environmentally friendly alternative for the recycling of organic wastes and its use is increasing in recent years. An exhaustive monitoring of the composting process and of the final compost characteristics is necessary to certify that the values of compost characteristics are within the limits established by the legislation in order to obtain a safe and marketable product. The analysis of these parameters on each composting batch in the commercial composting plant is time-consuming and expensive. So, their estimation in the composting facilities based on the use of near infrared reflectance spectroscopy (NIRS) could be an interesting approach in order to monitor compost quality. In this study, more than 300 samples from 20 different composting procedures were used to calibrate and validate the NIRS estimation of compost properties (pH, electrical conductivity (EC), total organic matter (TOM), total organic carbon (TOC), total nitrogen (TN) and C/N ratio, macronutrient contents (N, P, K) and potentially pollutant element concentrations (Fe, Cu, Mn and Zn)). The composts used were elaborated using different organic wastes from agroindustrial activities (GS: grape stalk; EGM: exhausted grape marc; GM: grape marc; V: vinasse; CJW: citrus juice waste; Alpeorujo: olive-oil waste; AS: almond skin; EP: exhausted peat; TSW: tomato soup waste; SMS: spent mushroom substrate) co-composted with manures (CM: cattle manure; PM: poultry manure) or urban wastes (SS: sewage sludge) The estimation results showed that the NIRS technique needs to be fitted to each element and property, using specific spectrum transformations, in order to achieve an acceptable accuracy in the prediction. However, excellent prediction results were obtained for TOM and TOC, successful calibrations for pH, EC, Fe and Mn, and moderately successful estimations for TN, C/N ratio, P, K, Cu and Zn.

The Challenge of Peat Substitution in Organic Seedling Production: Optimization of Growing Media Formulation through Mixture Design and Response Surface Analysis

Peat replacement is an increasing demand in containerized and transplant production, due to the environmental constraints associated to peat use. However, despite the wide information concerning the use of alternative materials as substrates, it is very complex to establish the best materials and mixtures. This work evaluates the use of mixture design and surface response methodology in a peat substitution experiment using two alternative materials (green compost and palm fibre trunk waste) for transplant production of tomato (Lycopersicon esculentum Mill.); melon, (Cucumis melo L.); and lettuce (Lactuca sativa L.) in organic farming conditions. In general, the substrates showed suitable properties for their use in seedling production, showing the best plant response the mixture of 20% green compost, 39% palm fibre and 31% peat. The mixture design and applied response surface methodology has shown to be an useful approach to optimize substrate formulations in peat substitution experiments to standardize plant responses.

Use of chemometrics in the chemical and microbiological characterization of composts from agroindustrial wastes

Physico-chemical, chemical and microbiological parameters were studied in a set of fifteen different composts elaborated with agroindustrial wastes using two different composting systems (turning and static pile composting). To carry out the chemometric evaluation, multivariate statistical analysis techniques, such as hierarchical cluster analysis (HCA) and factorial analysis (FA) were used. Composts obtained showed suitable physico-chemical and chemical properties for their use as organic amendment and a good maturity degree. HCA allowed to classify the organic materials mainly in four groups: cluster A, cluster B, cluster C and unclustered composts; also, this statistical tool showed the lack of influence of the composting system in the final characteristics of these composts. On the other hand, through FA, it was possible to identify the principal variables associated to the composting of agroindustrial wastes in four factors that explained 72.3% of the variability.

Study of the evolution of organic matter during composting of winery and distillery residues by classical and chemometric analysis.

The aim of the present paper is to evaluate the changes of organic matter during the composting process of fresh winery and distillery residues (WDR) by means of classical and chemometric analysis of (13)C cross-polarization magic angle spinning (CPMAS) NMR and Fourier transform infrared (FT-IR) spectra. (13)C NMR spectroscopy displayed a preferential biodegradation of carbohydrates as well as an accumulation of aliphatic chains (cutin- and suberin-like substances). This preferential biodegradation of the organic fractions reduces the landfill emission potential. Although the composition of the input mixture strongly affects the shape of the infrared (IR) spectra, typical bands of components can be selected and used to follow the composting process; that is, changes in the relative absorbances of the band of nitrate (at 1384 cm(-1)) and in the band of carbohydrates (at 1037 cm(-1)) have been observed. In addition, different chemometric tools, such as partial least-squares (PLS), interval PLS (iPLS), backward iPLS (biPLS), and genetic algorithm (GA), have been used to find the most relevant spectral region during the composting process. Chemometric analysis based on the combined and sequential use of iPLS and GA has been revealed as a very powerful tool for the detection in samples of the most relevant spectral region related to the composting process. From the obtained results, it can be concluded that CPMAS (13)C NMR supported by FT-IR could provide information about the evolution and characteristics of the organic matter during the composting process in order to avoid contamination problems after its use as amendment in agriculture or after landfilling.