Márcio de Carvalho Pires

Soil-Borne Plant Pathogens Associated to Declineof Grapevine Grown in Greenhouse

Soil-Borne Plant Pathogens Associated to Decline of Grapevine Grown in Greenhouse The purpose of the study was to determine which fungal species were associated with a decline in greenhouse grown grapevine (“Fujiminori”). Two species of fungi, characterized as soil-borne plant pathogens were isolated from roots. The fungi were identified by DNA sequencing methods and by their morphology. The fungi were identified as Cylindrocarpon destructans (FJMB2) and Fusarium Oxysporum (FJBM3) according to the DNA sequences and similarities after BLAST search.

Seasonal Variation of Plant Mineral Nutrition in Fruit Trees

This research evaluated the monthly variation of plant mineral nutrition in six species of fruit trees over a year. Leaf samples were taken from the fruit trees and nutritional HIGHLIGHTS • The currently research determined the seasonal variation of fruit trees mineral status

Optimum Level of Nitrogen and Phosphorus to Achieve Better Papaya (Carica papaya var. Solo) Seedlings Growth and Mycorrhizal Colonization

ABSTRACT An experiment under greenhouse conditions was carried out to evaluate the best level of nitrogen (N) and phosphorus (P), which promote the growth of papaya (Carica papaya L. var. Solo) seedlings without affecting the arbuscular mycorrhizal fungi (AMF) activity. Seedlings of papaya were transplanted to pots inoculated or not with Gigaspora margarita spores at four levels of N (0, 50, 100, and 200 ppm) and P (0, 25, 50, and 100 ppm). P was amended at the same time of pot filling up with substrate but N was applied weekly. The experiment was harvested after 100 days and plant dry weight together with AMF colonization was measured. The major response on the plant dry weight and height was from the fertilizers (N and P) where it proportionally increased. For the dry weight, at low amounts of nutrients the effects of AMF were greater, but as much as the fertilizer level in soil increased this response decreased. The colonization decreased as the nutrient levels increased, especially the P. The best combination of N and P for plant growth and AMF colonization could be at 50 and 25 ppm, equivalent to 5 and 2.5 kg/10a, respectively.