Maria de Fátima Malvar Gesteira

Clinical, hematological, and molecular characterization of sickle cell anemia pediatric patients from two different cities in Brazil.

This study focused on clinical, hematological, and molecular aspects of sickle cell anemia pediatric patients from two different cites in Brazil. Seventy-one patients from São Paulo and Salvador, aged 3 to 18 years, were evaluated. Hematological analyses, betaS globin gene haplotypes, and alpha2 3.7kb-thalassemia were performed. Numbers of hospitalizations due to vaso-occlusive crises, infections, stroke, and cholelithiasis were investigated. São Paulo had more hospitalizations from vaso-occlusion, cholelithiasis, and stroke than Salvador. The Ben/CAR genotype predominated in both cities. Alpha2 3.7kb-thalassemia had a frequency of 28.2% in Salvador, mostly with Ben/CAR genotype (45.0%), while São Paulo had 22.5% with similar frequencies of the Ben/ CAR and CAR/CAR genotypes. Sickle cell anemia patients from São Paulo also had more episodes of stroke, which was observed among CAR/CAR, atypical, and BEN/CAR haplotypes. In Salvador stroke was only observed in the Ben/CAR genotype. Cholelithiasis had similar frequencies in the two cities. These data suggest a milder phenotype among patients in Salvador, possibly due to genetic, environmental, and socioeconomic factors. Further studies are needed to elucidate modulating factors and phenotype association.

Estudo da Ação do Edta Sobre a Camada Residual no Tempo de 1 e 3 Minutos

Objeti vo: Analisar, in vitro, atraves da Microscopia Eletronica de Varredura, o efeito do EDTA nas concentracoes de 3%, 5%, 10% e 17%, na remocao da camada residual e na desobstrucao dos tubulos denti narios, nos tempos de 1 e 3 minutos. Metodo: Da amostra consti tuida de 80 dentes, foram selecionadas, randomicamente, oito unidades para compor o grupo controle negati vo (GC1) e 8 para consti tuirem o grupo controle positi vo (GC2). Os 64 restantes compuseram os oito Grupos Experimentais (GExp.). Apos a instrumentacao do canal radicular foi realizada a irrigacao fi nal com as solucoes de EDTA em teste, sendo considerados os tempos de 1 minuto e 3 minutos de permanencia deste em contato com a superfi cie denti naria. Resultados: A analise das fotomicrografi as com magnitude de 2000X, revelam que as solucoes de EDTA a 10% e 17% foram as mais efi cazes, independente dos tempos de aplicacao pre- determinados. Conclusao: A respeito da remocao da camada residual e da desobstrucao dos tubulos denti narios do canal radicular, que a efi cacia do EDTA nao se altera com o aumento do tempo de aplicacao nos tempos de 1 minuto ou 3 minutos.