Marília Cristina Sola

Real-time PCR detection of Brucella spp. DNA in lesions and viscera of bovine carcasses.

This study reports a real-time PCR assay for the detection of Brucella spp. associated with the FTA® Elute method in lesions observed during sanitary inspections in beef slaughter. Of the total 276 samples, 78 (28.3%) tested positive and 198 (71.7%) negative for Brucella spp. The real-time PCR technique associated with the FTA® Elute method proved to be an important tool for the diagnosis, judgment about and disposal of carcasses and viscera of slaughtered animals.

Identification of Salmonella sp. in Curraleiro Pé-Duro cattle raw milk

The microbiological quality of raw milk can be directly affected by animal health, hygiene procedures performed during milking and product storage condition. Faced with the need to characterize the microbiological aspects due to the milk production of cattle herd of CurraleiroPe-Duro breed, there were evaluated 226 samples of raw milk collected in the period of january 2013 to january 2014, for research of Salmonella sp.. The analytical tests were performed in Multi-User Laboratory of Microbiology Food Research Center at Veterinary Medicine School of the Federal University of Goias. According to the results in this study it was identified the presence of Salmonella sp., serovar Heidelberg and Schwarzengrund. Although this microorganism comprise the normal intestinal flora of humans and warm-blooded animals, be eliminated in feces and consequently promote environmental contamination and other animals, the identification of Salmonella in raw milk results the need for control in obtaining the milk process, storage and processing procedures of dairy products to ensure safety to consumers.