Massimo Nocentini

Bioremediation of a soil contaminated by hydrocarbon mixtures: the residual concentration problem

The phenomenon of residual concentration was investigated in the aerobic biodegradation of three different petroleum commercial products (i.e., kerosene, diesel fuel and a lubricating mineral oil) in static microcosms. Two different soils exhibiting different physical-chemical characteristics were used (i.e., a biologically treated hydrocarbon-contaminated soil and a pristine soil). Residual concentrations were observed and a simple way to take this phenomenon into account was proposed.

SOLIDS DISTRIBUTION IN STIRRED SLURRY REACTORS: INFLUENCE OF SOME MIXER CONFIGURATIONS AND LIMITS TO THE APPLICABILITY OF A SIMPLE MODEL FOR PREDICTIONS

Abstract The features of solids concentration distribution were investigated in baffled and unbaffled tanks of high aspect ratio, stirred with multiple radial, mixed-flow or axial impellers. In the baffled tanks the average profiles can be predicted with the sedimentation-dispersion model regardless of impeller type - in spite of slight systematic departures from the average trend at the impeller plane and/or midway between the impellers. This is hardly possible for the unbaffled tanks due to lack of physical foundation of this simple model. When the unbaffled tank is stirred with hydrofoil impellers, an inverted profile is even obtained. The mentioned departures and this last anomalous behaviour are qualitatively discussed with reference to particle-turbulence interaction.

Bioremediation of a polycyclic aromatic hydrocarbon‐contaminated soil by using different aerobic batch bioreactor systems

The intrinsic depuration capability of a soil contaminated by polycyclic aromatic hydrocarbons (PAHs) originating from a contaminated industrial site was evaluated in this study by using different aerobic batch bioreactors: a slurry‐phase bioreactor, a blade‐agitated bioreactor, and a rotary vessel bioreactor. For each bioreactor, the disappearance of 14 target PAHs and of the total extractable organic matter was monitored. The three treatments exhibited rapid and extensive removal of the PAHs, which disappeared at different degradation rates according to their molecular weight and aromaticity degree. PAHs with two, three, and four aromatic rings were degraded in sequence, with average rates that generally decreased as the number of molecule rings increased. A slight increase in the bacterial biomass concentration and significant CO2 production were also observed during the time course of the treatments. Among the three treatments, the slurry‐phase system provides the most effective and fastest removal of...

Aerobic/anaerobic/aerobic sequenced biodegradation of a mixture of chlorinated ethenes, ethanes and methanes in batch bioreactors.

A novel aerobic/anaerobic/aerobic treatment was implemented in batch reactors containing aquifer materials from a site contaminated by tetrachloroethylene (PCE), trichloroethylene (TCE), vinyl chloride (VC), 1,1,2-trichloroethane (1,1,2-TCA) and chloroform (CF). Consortia grown aerobically on methane, propane, n-pentane and n-hexane completely biodegraded the chlorinated solvent mixture, via aerobic cometabolism of VC, CF, TCE and 1,1,2-TCA, followed by PCE reductive dechlorination (RD) to 1,2-cis-dichlorothylene (cis-DCE) or TCE, and cis-DCE/TCE cometabolism in a further aerobic phase. n-Hexane was the best substrate. No electron donor was supplied for RD, which likely utilized cellular material produced during the aerobic phase. Chloride release was stoichiometric with chlorinated solvent biodegradation. According to the Lepidium sativum ecotoxicity test, a decreased toxicity was observed with propane, n-pentane and n-hexane, but not methane. A kinetic study of PCE RD allowed to estimate the PCE maximum specific rate (0.57 ± 0.07 mg mg(protein)(-1) day(-1)) and half-saturation constant (6.7 ± 1.5 mg L(-1)).

Dispersion coefficient and settling velocity of the solids in agitated slurry reactors stirred with multiple rushton turbines

Abstract The feature of solids distribution in tanks stirred with multiple Rushton turbines was investigated. Both transient and steady-state experiments were performed in tanks of two scales with a variety of suspensions. The data were analysed with the axial sedimentation–dispersion model. The axial dispersion coefficient of the solid phase was found not to differ from that of the liquid by more than 20%. The effective particle settling velocity in the stirred medium was then determined. It is confirmed that this parameter is different from the terminal settling velocity. Their ratio exhibits the same dependence on Kolmogoroff microscale and particle size as obtained previously with an indirect, approximate approach.

Biodegradation of PAHs in Aggregates of a Low Permeability Soil

In the bioremediation of low-permeability soils, pollutant and, especially, the oxygen bioavailability are often the rate limiting steps. In cases when a biopile treatment can represent an applicable technique, pretreatment of the excavated soil is often necessary to attain an adequate air-filled porosity in the soil and to avoid the presence of large soil aggregates. The present work was performed to evaluate the influence of soil aggregate size in the bioremediation of a silt-clay type soil contaminated by PAHs. Microcosms were arranged with spherical soil aggregates of different diameter in near water-saturation conditions. Concentration of two and three aromatic ring PAHs, total biom-ass, and respiration rates were monitored. PAH concentration profiles inside the particles were also obtained. A simple and quick way to estimate the critical dimension of the soil aggregates was developed based on the evaluation of an oxygen penetration depth, that is, the distance from the external surface to the aggregate core beyond which oxygen concentration is practically zero. A very different time course of PAHs consumption was found in the external layer and the inner core of the aggregates as well as in aggregates of different dimensions. The results suggest that only the 3 mm external layer of the sphere is not limited by oxygen diffusion.

Batch and Continuous Flow Adsorption of Phenolic Compounds from Olive Mill Wastewater: A Comparison between Nonionic and Ion Exchange Resins

The goals of this work were (i) to compare two anion ion exchange resins (IRA958 Cl and IRA67) and a nonionic resin (XAD16) in terms of phenolic compounds adsorption capacity from olive mill wastewater and (ii) to compare the adsorption capacity of the best resin on columns of different length. The ion exchange resins performed worse than nonionic XAD16 in terms of resin utilization efficiency (20% versus 43%) and phenolic compounds/COD enrichment factor (1.0 versus 2.5). The addition of volatile fatty acids did not hinder phenolic compounds adsorption on either resin, suggesting a noncompetitive adsorption mechanism. A pH increase from 4.9 to 7.2 did not affect the result of this comparison. For the best performing resin (XAD16), an increase in column length from 0.5 to 1.8 m determined an increase in resin utilization efficiency (from 12% to 43%), resin productivity (from 3.4 to 7.6 ), and phenolics/COD enrichment factor (from 1.2 to 2.5). An axial dispersion model with nonequilibrium adsorption accurately interpreted the phenolic compounds and COD experimental curves.

Solids Separation at the Exit of a Continuous-Flow Slurry Reactor Stirred with Multiple Axial Impellers

Measurements of solids concentration in both the withdrawal tube and upstream of the discharge opening were performed in a vessel stirred with multiple impellers and operated in a continuous mode. Apart from the influence of withdrawal velocity on the discharge concentration, which has been thoroughly described in the past, rotational speed plays a role on both concentrations. For the configuration studied, the ratio of the solids concentration in the discharge tube and upstream of the discharge is much lower than unity and can be simply correlated to the operating conditions.