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Dive into the research topics where Misao Kojima is active.

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Featured researches published by Misao Kojima.


Journal of Fermentation Technology | 1986

Purification and Some Properties of an Esterase from Yeast

Noriko Toshimitsu; Hiroshi Hamada; Misao Kojima

Abstract An esterase was isolated and purified from bakers yeast by ammonium sulfate precipitation and column chromatographies on Sephacryl S-200, DEAE-Sephacel, chromatofocusing, and DEAE-Sephacel again. The molecular weight of the enzyme was approximately 84,000 on Sephadex G-100 and 40,000 by sodium dodecyl sulfate-poly-acrylamide gel electrophoresis, suggesting a dimer for the activity. This enzyme hydrolyzed short-chain naphthyl esters and p -nitrophenyl esters, and its activity was strongly inhibited by mercuric compounds. The esterase appeared to be an arylesterase (EC 3.1.1.2) and its optimum pH was 8.0 at 30°C.


Agricultural and biological chemistry | 1990

Function of Cupric Ion in the Breakage of pBR322 ccc-DNA by D-Glucosamine

Nobuhiro Kashige; Misao Kojima; Yukihiko Nakashima; Kenji Watanabe; Ayako Tachifuji


NIPPON SHOKUHIN KOGYO GAKKAISHI | 1982

Studies on the Stability of Dried Wasabi Flour

Misao Kojima; Hiroshi Hamada; Miyuki Yamashita


Agricultural and biological chemistry | 1991

Correlation between DNA-Breaking Activity of Aminosugars and the Amounts of Active Oxygen Molecules Generated in Their Aqueous Solutions

Nobuhiro Kashige; Misao Kojima; Kenji Watanabe


Journal of Food Science and Technology-mysore | 1990

Simultaneous Quantitative Determination of Seven Isothiocyanates by Gas Chromatography Equipped with FPD

Misao Kojima; Kohichi Yanaga; Hiroshi Hamada; Nobuhiro Kashige


Journal of Food Science and Technology-mysore | 1986

Comparison of Volatile Sulfur Compounds in the Hydrolysates of Wasabi, Horseradish and Black Mustard on the Market

Misao Kojima; Hiroshi Hamada; Noriko Toshimitsu


Agricultural and biological chemistry | 1990

Specificity of Nucleotide Sequence in DNA Cleavage Induced by D-Glucosamine and D-Glucosamine-6-phosphate in the Presence of Cu2+

Kenji Watanabe; Nobuhiro Kashige; Misao Kojima; Yukihiko Nakashima


Journal of Pharmaceutical Sciences | 1976

Antimicrobial Activity of Newly Synthesized Isothiocyanate Derivatives Against Pathogenic Plant Microorganisms

M. Kado; Misao Kojima


Journal of Food Science and Technology-mysore | 1988

Studies on the Mixing Ratios of Wasabiand Horseradish in the Pastes

Misao Kojima; Kohichi Yanaga; Hiroshi Hamada; Nobuhiro Kashige


Journal of Food Science and Technology-mysore | 1985

Changes in Isothiocyanates of Wasabia japonica Roots by Drying

Misao Kojima; Hiroshi Hamada; Noriko Toshimitsu

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