Mohammad Juffrie

Prevalence and Management of Functional Gastrointestinal Disorders in Infants: An Asian Perspective

Xin Hua Hospital, Shanghai Jia Tong University School of Medicine, Shanghai, China, *Santokba Durlabhji Memorial Hospital, Jaipur, India, Department of Pediatrics, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia,Department of Paediatrics, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia, Department of Paediatrics, National University Hospital, Singapore, Siriraj Hospital, Mahidol University, Bangkok, Thailand, Nutricia Research, Utrecht, The Netherlands, **Nutricia Research, Singapore

The Effect of Overnutrition Toward the Risk of Dengue Shock Syndrome in Pediatric Patient: In-Depth Investigation of sVCAM-1 and Adiponectin Level

Introduction: Overnutrition has become an emergent health problem worldwide and could significantly alter the normal human physiology. It still unknown how overnutrition could affect the outcome of pediatric patient with dengue infection. This study aimed to unveil how overnutrition affects the risk of dengue shock syndrome (DSS) in dengue pediatric patient by evaluating the adiponectin which has immune-modulatory property and the inflammation

Perbedaan Kadar Interferon Gamma pada Tuberkulosis Anak

Latar belakang . Tuberkulosis (TB) adalah penyakit akibat infeksi kuman Mycobacterium tuberculosis, suatu bakteri batang Gram positif. Salah satu sitokin yang diproduksi sel Th1 adalah interferon gamma (IFN-γ) yang berperan penting dalam mengeliminasi bakteri Mycobacterium tuberculosis. Terjadinya gangguan atau penurunan aktivitas sel Th1 dan sitokinnya yaitu IFN-γ cukup bermakna dalam memengaruhi mekanisme pertahanan tubuh terhadap penyakit TB. Manifestasi klinis penyakit TB terjadi karena adanya defisiensi imun, terutama imunitas selular. Tujuan . Mengkaji perbedaan kadar interferon gamma dilihat dari derajat lesi paru pada pasien TB anak. Metode . Penelitian cross sectional dilakukan di RSUP Dr. Sardjito and RSUD Sleman selama bulan Desember 2014. Subyek penelitian adalah anak kurang dari 15 tahun yang terdiagnosis TB menggunakan skor TB IDAI. Produksi interferon-gamma diukur dengan metode ELISA dan perbedaan kadarnya dibandingkan dengan derajat lesi paru. Hasil . Berdasarkan derajat lesi paru, kadar IFN-γ pada kasus tuberkulosis anak dengan lesi paru minimal (8,37±3,25) lebih tinggi daripada kasus dengan lesi paru sedang (3,52±1,75), dan lesi paru luas (4,83±2,78). Kesimpulan . Ada perbedaan rerata kadar IFN-γ serum TB anak berdasarkan derajat lesi paru minimal, sedang, dan luas, walaupun secara statistik tidak bermakna.

Effect of dengue virus infection on the permeability of vero cells line

Vero cell line is widely used in many in vitro studies including dengue infection studies. Recent study explore the increase of permeability of vero cell line due to infection dengue virus serotype 2. Vero cell line (CCL-81, ATCC) cultured in the transwell polyester permeable membrane and infected by dengue virus-2. Culture media containing albumin was used to assess the transvero albumin permeability. The content of albumin migrates across the vero cells was determined using protein assay dye reagent and BSA standard (5 and 10 µg/μl), and then measured by microplate reader. The results indicate that dengue infection increases the permeability of vero cells with significant value compared to the control, especially at two days and more post infection. The result of the study suggests that vero cell line can be used as model cells in the permeability studies of infection of dengue virus serotype 2.