Mustafa Akil

Effect of zinc supplementation on lipid peroxidation and lactate levels in rats with diabetes induced by streptozotocin and subjected to acute swimming exercise.

OBJECTIVE The present study aims to explore the effect of zinc supplementation on lipid peroxidation and lactate levels in rats having diabetes induced by streptozotocin and subjected to acute swimming exercise. METHOD A total of 80 adult male rats of Sprague-Dawley type were equally allocated to 8 groups: Group 1, general control. Group 2, zinc-supplemented group. Group 3, zinc-supplemented, diabetic group. Group 4, swimming control group. Group 5, zinc-supplemented swimming group. Group 6, zinc-supplemented diabetic swimming group. Group 7, diabetic swimming group. Group 8, diabetic group. At the end of the 4-week study, blood samples were collected to determine MDA, GSH, GPx, SOD, lactate and zinc levels. RESULTS The highest MDA values were found in group 7 and 8 (p<0.001). GSH values in groups 5 and 6 were higher (p<0.001). The highest GPx values were established in groups 2, 5 and 6 (p<0.001). SOD values were the highest in groups 5 and 6 (p<0.001) and lowest in groups 2, 3 and 8 (p<0.001). The highest plasma lactate levels were found in group 7 (p<0.001). The highest zinc levels were obtained in groups 1, 2 and 5 (p<0.001), and the lowest zinc levels were found in groups 7 and 8 (p<0.001). CONCLUSION Results of the study reveal that zinc supplementation prevents the increase of free radical formation, suppression of antioxidant activity and muscle exhaustion, all of which result from diabetes and acute exercise. Zinc supplementation may contribute to health performance in diabetes and acute exercise (Tab. 2, Fig. 1 Ref. 47). Full Text in PDF www.elis.sk.

The effect of selenium supplementation on elements distribution in liver of rats subject to strenuous swimming.

The present study aims to explore how selenium supplementation affects the element distribution in the liver tissue of rats subjected to strenuous swimming exercise. Thirty-two Spraque-Dawley male rats were equally divided into the four groups: Group 1, normal control group. Group 2, selenium-supplemented, non-swimming (0.6 mg/kg/day sodium selenite) group. Group 3, swimming, no supplementation group. Group 4, swimming, selenium-supplemented (0.6 mg/kg/day sodium selenite) group. After one month, the animals were decapitated and liver tissue samples were collected to determine the levels of lead, cobalt, boron, molybdenum, chromium, sulfur, magnesium, sodium, potassium, phosphorus, copper, iron, zinc and selenium. The chromium, molybdenum, iron, sodium and potassium values were higher in the swimming groups, relative to controls. Group 3 had significantly lower lead levels (p<0.001). The highest cobalt levels were obtained in the Group 1 and that of the Group 2 was higher than in the Groups 3 and 4. The boron values in the Group 3 were higher than those in all other groups. The copper and magnesium levels were higher in the Groups 3 and 4, compared to the Groups 1 and 2. The highest phosphorus levels were found in the Group 1. The highest selenium and zinc values were obtained in the Group 2 and those of the Group 4 were higher than in the Groups 1 and 3. Group 1 had higher selenium and zinc levels than the Group 3. The results of the present study demonstrated that selenium-supplemented rats subjected to strenuous swimming exercise had distinct elements distribution in liver tissue. Also, selenium supplementation offsets the decrease in zinc levels in rats subjected to vigorous swimming (Tab. 3, Ref. 20).

Effect of melatonin on element distribution in the liver tissue of diabetic rats subjected to forced exercise.

The objective of the present study was to investigate the effects of melatonin supplementation on elements in the liver of diabetic rats subjected to acute swimming exercise. Eighty adult male rats were equally divided into eight groups. Group 1, general control. Group 2, melatonin-supplemented control. Group 3, melatonin-supplemented diabetic control. Group 4, swimming control. Group 5, melatonin-supplemented swimming. Group 6, melatonin-supplemented diabetic swimming. Group 7, diabetic swimming. Group 8, diabetic control. Liver tissue samples were analyzed for lead, cobalt, molybdenum, chrome, sulphur, magnesium, manganese, sodium, potassium, phosphorus, copper, iron, calcium, zinc, selenium. The highest cobalt, chrome values were found in the groups 7, 8 and the groups 5, 6 respectively. Groups 3 and 7 had the highest copper values. Iron and potassium values were higher in the groups 1 and 4. Group 6 had increased magnesium value, and groups 6, 7, 8 were found to have the highest manganese levels. The highest lead values were found in the groups 5 and 6. Group 6 had the highest selenium levels. The highest zinc levels were established in 1 and 2. Groups 1, 2, 5 and 6 were found to have the highest calcium values. The results of our study indicate that melatonin supplementation in diabetes and forced exercise significantly alters the element metabolism in the liver (Tab. 3,Ref. 33).

Selenium prevents lipid peroxidation in liver and lung tissues of rats in acute swimming exercise.

BACKGROUND Selenium, which is critical for human health, is necessary for various metabolic processes, including thyroid hormone metabolism, protection against oxidative stress, and immune function. OBJECTIVE The present study aims to examine how selenium administration affects lipid peroxidation in liver and lung tissues of rats subjected to acute swimming exercise. METHODS The study included 32 Spraque-Dawley adult male rats divided into Group 1 (general control), Group 2 (selenium-administered), Group 3 (swimming), and Group 4 (selenium-administered swimming). MDA and GSH levels were determined in liver and lung tissues. RESULTS The highest MDA values in the liver and lung tissues were found in group 3 in the study. MDA value in group 4 was higher than those in groups 1 and 2. Group 4 had the highest liver and lung GSH levels. GSH levels in Group 3 were higher than those in groups 1 and 2. CONCLUSION Results of the study indicate that acute swimming exercise causes lipid peroxidation in liver and lung tissues, while selenium administration prevents free radical formation by increasing antioxidant activity (Tab. 2, Ref. 26).

Protective effect of melatonin on lipid peroxidation in various tissues of diabetic rats subjected to an acute swimming exercise.

BACKGROUND The present study aimed to explore the effect of melatonin administration on lipid peroxidation in various tissues of rats with streptozocin induced diabetes and subjected to an acute swimming exercise. METHODS The study used 80 adult male rats, which were equally allocated to 8 groups: Group 1, general control; Group 2, melatonin-administered control; Group 3, melatonin-administered diabetic control; Group 4, swimming control; Group 5, melatonin-administered swimming; Group 6, melatonin-administered diabetic swimming; Group 7, diabetic swimming; Group 8, diabetic control. Diabetic rats were administered 3 mg/kg/day ip melatonin for 4 weeks. At the end of the study, the animals were decapitated to collect samples from liver, lung and spleen tissues, which were then analyzed to determine levels of liver MDA (nmol/gram/protein) and GSH (mg/g/protein). RESULTS The highest MDA values in liver, lung and spleen tissues were obtained in the Group 7. The values in the Group 8 were lower than those in the Group 7, but higher than in all other groups. The Group 5 and 6 had the highest liver, lung and spleen GSH values. CONCLUSION Results obtained from the study indicate that the increase in free radical production and the inhibition of antioxidant activity in diabetes and acute exercise are both prevented by melatonin administration (Tab. 2, Ref. 30).

Effects of a 3-month recreative exercise applied to individuals with intellectual disability on their electromyogram (EMG) variations and balance performance

Objective: The objective of the present study was to determine how 3-month recreative exercises affect the balance performance of individuals with intellectual disability (ID) and to evaluate the electromyogram (EMG) changes following exercises. Methods: Eleven individuals (M=14.18, S.D=1.94) with mild intellectual disability (MID) who attend the school for children with ID and to the vocational school were included in the study. In the study, the subjects were applied a recreative exercise program (60 min/3 days/12 weeks) and their balance, EMG, and aerobic and anaerobic performances were evaluated by means of the pretest-posttest model. Results: When the EMG values in our study were examined, differences were found in terms of EMG3-VL muscle variable (P=0.075/2=0.038), EMG3-RF muscle variable (P=0.080/2=0.040), and EMG5-RF muscle variable (P=0.066/2=0.033). The exercise program decreased the EMG values significantly. When the balance scores were evaluated, significant differences were found in terms of the variables of Balance 2 (after 30 s) (P=0.077/2=0.039) and Balance 3 (after 3 min) (P=0.084/2=0.042). The exercise program significantly decreased the balance values. Conclusion: Consequently, it was determined that 3-month recreative exercises applied to individuals with ID lowered the EMG and Balance values during and after the exercise. In this context, it is thought that the people working with the children with MID need to consider the parameters constituting the balance in a detailed way in their exercise practices and to include activities improving their balance skills into their programs, and that recreative exercises can also provide contribution in this term.