Naohiro Oikawa

Immunohistochemical demonstration of papillomavirus antigen in cervical dysplasia and vulvar condyloma

Biopsies from 30 cases of vulvar condyloma, 460 cases of cervical dysplasia, 30 cases of carcinoma in situ and 75 cases of invasive carcinoma of the cervix were screened for the presence of human papillomavirus (HPV) antigen by means of the peroxidase-antiperoxidase method. Positive reaction for HPV was detected in 14 cases of condyloma and 80 cases of dysplasia as a brown intranuclear precipitate in the superficial layer of the epithelium. None of the cases of carcinoma in situ and invasive cancer were positive for HPV. The mean age of the women with HPV-positive dysplasia was significantly lower than that of the women with HPV-negative dysplasia. Condylomatous dysplasia showed a significantly higher positive rate than did noncondylomatous dysplasia. Cases of condylomatous dysplasia with severe stromal inflammation were negative for HPV more frequently than those with mild stromal inflammation.

Cytologic patterns of the cervical dysplasia with papillomavirus antigen.

子宮頸癌検診で発見された異型上皮のなかで, 免疫ペルオキシダーゼ法によって, 組織切片中にpapillomavirus antigenの証明された症例の細胞像を検討した.対象は, HPV陽性異型上皮80例で, 初回スクリーニング時のパパニコロ染色標本を検索した. HPV感染に特徴的な, koilocyte, dyskeratocyte, condylomatous parabasal cellの3種類の細胞の出現を中心に検討を加え, 以下の結果を得た.1) 3種の細胞の出現頻度は, koilocyte46例 (58%), dyskeratocyte29例 (36%), condylornatousparabasal cell56例 (70%) であった. 各細胞の出現パターンに, 一定の傾向は認められなかった.2) 出現細胞によって各症例を分類すると,(a) ほとんどコンジローマ性細胞,(b) コンジローマ性細胞優位で異型上皮細胞混在,(c) 異型上皮細胞優位でコンジローマ性細胞混在, の3群に大別され,(a)-(c) の症例数は, ほぼ同じ割合であった.3) 2核細胞は50例 (63%) に認められた.