Naoto Nemoto
Mitsubishi
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Publication
Featured researches published by Naoto Nemoto.
FEBS Letters | 1997
Naoto Nemoto; Etsuko Miyamoto-Sato; Yuzuru Husimi; Hiroshi Yanagawa
Adequate means for genotype assignment to phenotype is essential in evolutionary molecular engineering. In this study, construction of ‘in vitro virus’ was carried out in which a genotype molecule (mRNA) covalently binds to the phenotype molecule (protein) through puromycin on the ribosome in a cell‐free translation system. Bonding efficiency was ∼10%, thus indicating a population of the in vitro virus to have ∼1012 protein variants, this number being 104 that in the phage display. The in vitro virus is useful for examining protein evolution in a test tube and the results may possibly serve as basis for a general method for selecting proteins possessing the most desirable functions.
FEBS Letters | 1999
Naoto Nemoto; Etsuko Miyamoto-Sato; Hiroshi Yanagawa
We have developed a new method for the C‐terminus‐specific fluorescence labeling of proteins. This method is based on the experimental finding that a fluorescent puromycin analogue at lower concentrations bonds efficiently to the C‐terminus of mature proteins in cell‐free translation systems using mRNA without a stop codon. This labeling is performed under moderate conditions and its labeling efficiency is in the range of 50–95%. Here we demonstrate a protein‐protein interaction assay using fluorescence polarization measurement. This labeling method should also be useful for other rapid molecular interaction assays without purification of the labeled proteins, such as fluorescence correlation spectroscopy.
Archive | 1997
Hiroshi Yanagawa; Naoto Nemoto; Etsuko Miyamoto; Yuzuru Husimi
Nucleic Acids Research | 2000
Etsuko Miyamoto-Sato; Naoto Nemoto; Kensei Kobayashi; Hiroshi Yanagawa
Archive | 2000
Hiroshi Yanagawa; Naoto Nemoto
Archive | 2001
Hiroshi Yanagawa; Naoto Nemoto; Etsuko Miyamoto
Archive | 1998
Naoto Nemoto; Hiroshi Yanagawa; 弘志 柳川; 直人 根本
Archive | 2001
Takashi Funatsu; Naoto Nemoto; 直人 根本; 高志 船津
Archive | 2002
Naoto Nemoto; Susumu Sasaki; Yoshikazu Shiratori; Ichiro Tabuchi; 享 佐々木; 直人 根本; 一郎 田渕; 美和 白鳥
Archive | 2001
Hiroshi Yanagawa; Nobuhide Doi; Naoto Nemoto