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Dive into the research topics where Neil A. Youngson is active.

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Featured researches published by Neil A. Youngson.


Annual Review of Genomics and Human Genetics | 2008

Transgenerational Epigenetic Effects

Neil A. Youngson; Emma Whitelaw

Transgenerational epigenetic effects include all processes that have evolved to achieve the nongenetic determination of phenotype. There has been a long-standing interest in this area from evolutionary biologists, who refer to it as non-Mendelian inheritance. Transgenerational epigenetic effects include both the physiological and behavioral (intellectual) transfer of information across generations. Although in most cases the underlying molecular mechanisms are not understood, modifications of the chromosomes that pass to the next generation through gametes are sometimes involved, which is called transgenerational epigenetic inheritance. There is a trend for those outside the field of molecular biology to assume that most cases of transgenerational epigenetic effects are the result of transgenerational epigenetic inheritance, in part because of a misunderstanding of the terms. Unfortunately, this is likely to be far from the truth.


Developmental Cell | 2008

A Maternal-Zygotic Effect Gene, Zfp57, Maintains Both Maternal and Paternal Imprints

Xiajun Li; Mitsuteru Ito; Fen Zhou; Neil A. Youngson; Xiaopan Zuo; Philip Leder; Anne C. Ferguson-Smith

The mechanisms responsible for maintaining genomic methylation imprints in mouse embryos are not understood. We generated a knockout mouse in the Zfp57 locus encoding a KRAB zinc finger protein. Loss of just the zygotic function of Zfp57 causes partial neonatal lethality, whereas eliminating both the maternal and zygotic functions of Zfp57 results in a highly penetrant embryonic lethality. In oocytes, absence of Zfp57 results in failure to establish maternal methylation imprints at the Snrpn imprinted region. Intriguingly, methylation imprints are reacquired specifically at the maternally derived Snrpn imprinted region when the zygotic Zfp57 is present in embryos. This suggests that there may be DNA methylation-independent memory for genomic imprints. Zfp57 is also required for the postfertilization maintenance of maternal and paternal methylation imprints at multiple imprinted domains. The effects on genomic imprinting are consistent with the maternal-zygotic lethality of Zfp57 mutants.


Nature Genetics | 2003

Imprinted microRNA genes transcribed antisense to a reciprocally imprinted retrotransposon-like gene

Hervé Seitz; Neil A. Youngson; Shau-Ping Lin; Simone Dalbert; Martina Paulsen; Jean-Pierre Bachellerie; Anne C. Ferguson-Smith; Jérôme Cavaillé

MicroRNAs (miRNAs) are an abundant class of RNAs that are ∼21–25 nucleotides (nt) long, interact with mRNAs and trigger either translation repression or RNA cleavage (RNA interference, RNAi) depending on the degree of complementarity with their targets. Here we show that the imprinted mouse distal chromosome 12 locus encodes two miRNA genes expressed from the maternally inherited chromosome and antisense to a retrotransposon-like gene (Rtl1) expressed only from the paternal allele.


Nature Genetics | 2007

Modifiers of epigenetic reprogramming show paternal effects in the mouse

Suyinn Chong; Nicola Vickaryous; Alyson Ashe; Natasha Zamudio; Neil A. Youngson; Sarah J. Hemley; Tomas Stopka; Arthur I. Skoultchi; Jacqui Matthews; Hamish S. Scott; David M. de Kretser; Moira K. O'Bryan; Marnie E. Blewitt; Emma Whitelaw

There is increasing evidence that epigenetic information can be inherited across generations in mammals, despite extensive reprogramming both in the gametes and in the early developing embryo. One corollary to this is that disrupting the establishment of epigenetic state in the gametes of a parent, as a result of heterozygosity for mutations in genes involved in reprogramming, could affect the phenotype of offspring that do not inherit the mutant allele. Here we show that such effects do occur following paternal inheritance in the mouse. We detected changes to transcription and chromosome ploidy in adult animals. Paternal effects of this type have not been reported previously in mammals and suggest that the untransmitted genotype of male parents can influence the phenotype of their offspring.


Genome Biology | 2013

Redistribution of H3K27me3 upon DNA hypomethylation results in de-repression of Polycomb target genes

James P. Reddington; Sara M Perricone; Colm E. Nestor; Judith Reichmann; Neil A. Youngson; Masako Suzuki; Diana Reinhardt; Donncha S. Dunican; James Prendergast; Heidi K. Mjoseng; Bernard Ramsahoye; Emma Whitelaw; John M. Greally; Ian R. Adams; Wendy A. Bickmore; Richard R. Meehan

BackgroundDNA methylation and the Polycomb repression system are epigenetic mechanisms that play important roles in maintaining transcriptional repression. Recent evidence suggests that DNA methylation can attenuate the binding of Polycomb protein components to chromatin and thus plays a role in determining their genomic targeting. However, whether this role of DNA methylation is important in the context of transcriptional regulation is unclear.ResultsBy genome-wide mapping of the Polycomb Repressive Complex 2-signature histone mark, H3K27me3, in severely DNA hypomethylated mouse somatic cells, we show that hypomethylation leads to widespread H3K27me3 redistribution, in a manner that reflects the local DNA methylation status in wild-type cells. Unexpectedly, we observe striking loss of H3K27me3 and Polycomb Repressive Complex 2 from Polycomb target gene promoters in DNA hypomethylated cells, including Hox gene clusters. Importantly, we show that many of these genes become ectopically expressed in DNA hypomethylated cells, consistent with loss of Polycomb-mediated repression.ConclusionsAn intact DNA methylome is required for appropriate Polycomb-mediated gene repression by constraining Polycomb Repressive Complex 2 targeting. These observations identify a previously unappreciated role for DNA methylation in gene regulation and therefore influence our understanding of how this epigenetic mechanism contributes to normal development and disease.


Journal of Molecular Evolution | 2005

A Small Family of Sushi-Class Retrotransposon-Derived Genes in Mammals and Their Relation to Genomic Imprinting

Neil A. Youngson; Sylvia Kocialkowski; Nina Peel; Anne C. Ferguson-Smith

Ty3/gypsy retrotransposons are rare in mammalian genomes despite their abundance in invertebrate and other vertebrate classes. Here we identify a family of nine conserved mammalian genes with homology to Ty3/gypsy retrotransposons but which have lost their ability to autonomously retrotranspose. Of these, five map to the X chromosome while the remaining four are autosomal. Comparative phylogenetic analyses show them to have strongest homology to the sushi-ichi element from Fugu rubripes. Two of the autosomal gene members, Peg10 and Rtl1, are known to be imprinted, being expressed from the paternally inherited chromosome homologue. This suggests, consistent with the host-parasite response theory of the evolution of the imprinting mechanism, that parental-origin specific epigenetic control may be mediated by genomic “parasitic” elements such as these. Alternatively, these elements may preferentially integrate into regions that are differentially modified on the two homologous chromosomes such as imprinted domains and the X chromosome and acquire monoallelic expression. We assess the imprinting status of the remaining autosomal members of this family and show them to be biallelically expressed in embryo and placenta. Furthermore, the methylation status of Rtl1 was assayed throughout development and was found to resemble that of actively, silenced repetitive elements rather than imprinted sequences. This indicates that the ability to undergo genomic imprinting is not an inherent property of all members of this family of retroelements. Nonetheless, the conservation but functional divergence between the different members suggests that they have undergone positive selection and acquired distinct endogenous functions within their mammalian hosts.


The FASEB Journal | 2014

Paternal high-fat diet consumption induces common changes in the transcriptomes of retroperitoneal adipose and pancreatic islet tissues in female rat offspring

Sheau-Fang Ng; Ruby C.Y. Lin; Christopher A. Maloney; Neil A. Youngson; Julie A. Owens; Margaret J. Morris

We previously showed that paternal high‐fat diet (HFD) consumption programs β‐cell dysfunction in female rat offspring, together with transcriptome alterations in islets. Here we investigated the retroperitoneal white adipose tissue (RpWAT) transcriptome using gene and pathway enrichment and pathway analysis to determine whether commonly affected network topologies exist between these two metabolically related tissues. In RpWAT, 5108 genes were differentially expressed due to a paternal HFD; the top 5 significantly enriched networks identified by pathway analysis in offspring of HFD fathers compared with those of fathers fed control diet were: mitochondrial and cellular response to stress, telomerase signaling, cell death and survival, cell cycle, cellular growth and proliferation, and cancer. A total of 187 adipose olfactory receptor genes were down‐regulated. Interrogation against the islet transcriptome identified specific gene networks and pathways, including olfactory receptor genes that were similarly affected in both tissues (411 common genes, P<0.05). In particular, we highlight a common molecular network, cell cycle and cancer, with the same hub gene, Myc, suggesting early onset developmental changes that persist, shared responses to programmed systemic factors, or crosstalk between tissues. Thus, paternal HFD consumption triggers unique gene signatures, consistent with premature aging and chronic degenerative disorders, in both RpWAT and pancreatic islets of daughters.—Ng, S.‐F., Lin, R. C. Y., Maloney, C. A., Youngson, N. A., Owens, J. A., Morris, M. J. Paternal high‐fat diet consumption induces common changes in the transcriptomes of retroperitoneal adipose and pancreatic islet tissues in female rat offspring. FASEB J. 28, 1830–1841 (2014). www.fasebj.org


Philosophical Transactions of the Royal Society B | 2012

What obesity research tells us about epigenetic mechanisms

Neil A. Youngson; Margaret J. Morris

The pathophysiology of obesity is extremely complex and is associated with extensive gene expression changes in tissues throughout the body. This situation, combined with the fact that all gene expression changes are thought to have associated epigenetic changes, means that the links between obesity and epigenetics will undoubtedly be vast. Much progress in identifying epigenetic changes induced by (or inducing) obesity has already been made, with candidate and genome-wide approaches. These discoveries will aid the clinician through increasing our understanding of the inheritance, development and treatment of obesity. However, they are also of great value for epigenetic researchers, as they have revealed mechanisms of environmental interactions with epigenetics that can produce or perpetuate a disease state. Here, we will review the evidence for four mechanisms through which epigenetics contributes to obesity: as downstream effectors of environmental signals; through abnormal global epigenetic state driving obesogenic expression patterns; through facilitating developmental programming and through transgenerational epigenetic inheritance.


Biological Chemistry | 2004

Imprinted small RNA genes.

Hervé Seitz; Hélène Royo; Shau-Ping Lin; Neil A. Youngson; Anne C. Ferguson-Smith; Jérôme Cavaillé

Abstract Genomic imprinting is an epigenetic phenomenon that results in differential expression of both alleles, depending on their parent of origin. We have recently identified many imprinted small non-coding RNA genes belonging to the C/D RNA and microRNA gene families, both of which are usually known to play key roles in post-transcriptional metabolism of specific genes (e.g. C/D RNAs guide ribose methylation of target RNAs while microRNAs elicit either translational repression or RNA interference). Although the functional and evolutionary significance of this association between C/D RNA genes, microRNA genes and genomic imprinting is still highly elusive, these observations provide a framework for further analysis of the potential role of small non-coding RNAs in epigenetic control.


Proceedings of the National Academy of Sciences of the United States of America | 2013

Reintroducing domesticated wild mice to sociality induces adaptive transgenerational effects on MUP expression

Adam C. Nelson; Joseph W. Cauceglia; Seth D. Merkley; Neil A. Youngson; Andrew J. Oler; Randy J. Nelson; Bradley R. Cairns; Emma Whitelaw; Wayne K. Potts

Significance When wild-derived laboratory mice are reintroduced to socially competitive populations, they quickly adapt by producing attractive sons that otherwise have no fitness advantages, consistent with the sexy sons model of sexual selection. These attractive sons inherit up-regulated expression of several pheromones belonging to the major urinary protein (MUP) family. Up-regulation is controlled by maternal social experience, and is associated with epigenetic modifications of MUP promoters that could enhance transcription. Inheritance of up-regulated MUPs is likely adaptive because females have odor preferences for male scent marks with higher MUP concentration. These results represent one of only a few cases where parental social experience adaptively modifies progeny phenotype. When brought into captivity, wild animals can adapt to domestication within 10 generations. Such adaptations may decrease fitness in natural conditions. Many selective pressures are disrupted in captivity, including social behavioral networks. Although lack of sociality in captivity appears to mediate domestication, the underlying mechanisms are not well understood. Additionally, determining the contribution of genetic inheritance vs. transgenerational effects during relaxed selection may provide insight into the flexibility of adaptation. When wild-derived mice kept under laboratory conditions for eight generations were reintroduced to sociality and promiscuity (free mate choice), they adapted within two generations. Fitness assessments between this promiscuous lineage and a monogamous laboratory lineage revealed male-specific effects. Promiscuous-line males had deficits in viability, but a striking advantage in attracting mates, and their scent marks were also more attractive to females. Here, we investigate mechanistic details underlying this olfactory signal and identify a role of major urinary protein (MUP) pheromones. Promiscuous-line males inherit higher MUP expression than monogamous-line males through transgenerational inheritance. Sociality-driven maternal and paternal effects reveal intriguing conflicts among parents and offspring over pheromone expression. MUP up-regulation is not driven by hormone-driven transduction pathways, but rather is associated with reduction in DNA methylation of a CpG dinucleotide in the promoter. This reduction in methylation could enhance transcription by promoting the binding of transcription factor USF1 (upstream stimulatory factor 1). Finally, we experimentally demonstrate that increased MUP expression is a female attractant. These results identify molecular mechanisms guiding domestication and adaptive responses to fluctuating sociality.

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Emma Whitelaw

QIMR Berghofer Medical Research Institute

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Margaret J. Morris

University of New South Wales

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Shau-Ping Lin

Mackay Memorial Hospital

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Suyinn Chong

QIMR Berghofer Medical Research Institute

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Golam Mezbah Uddin

University of New South Wales

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J. William O. Ballard

University of New South Wales

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