Neil Kelly

Examination of the efficacy of acute L-alanyl-L-glutamine ingestion during hydration stress in endurance exercise

BackgroundThe effect of acute L-alanyl-L-glutamine (AG; Sustamine™) ingestion on performance changes and markers of fluid regulation, immune, inflammatory, oxidative stress, and recovery was examined in response to exhaustive endurance exercise, during and in the absence of dehydration.MethodsTen physically active males (20.8 ± 0.6 y; 176.8 ± 7.2 cm; 77.4 ± 10.5 kg; 12.3 ± 4.6% body fat) volunteered to participate in this study. During the first visit (T1) subjects reported to the laboratory in a euhydrated state to provide a baseline (BL) blood draw and perform a maximal exercise test. In the four subsequent randomly ordered trials, subjects dehydrated to -2.5% of their baseline body mass. For T2, subjects achieved their goal weight and were not rehydrated. During T3 - T5, subjects reached their goal weight and then rehydrated to 1.5% of their baseline body mass by drinking either water (T3) or two different doses (T4 and T5) of the AG supplement (0.05 g·kg-1 and 0.2 g·kg-1, respectively). Subjects then exercised at a workload that elicited 75% of their VO2 max on a cycle ergometer. During T2 - T5 blood draws occurred once goal body mass was achieved (DHY), immediately prior to the exercise stress (RHY), and immediately following the exercise protocol (IP). Resting 24 hour (24P) blood samples were also obtained. Blood samples were analyzed for glutamine, potassium, sodium, aldosterone, arginine vasopressin (AVP), C-reactive protein (CRP), interleukin-6 (IL-6), malondialdehyde (MDA), testosterone, cortisol, ACTH, growth hormone and creatine kinase. Statistical evaluation of performance, hormonal and biochemical changes was accomplished using a repeated measures analysis of variance.ResultsGlutamine concentrations for T5 were significantly higher at RHY and IP than T2 - T4. When examining performance changes (difference between T2 - T5 and T1), significantly greater times to exhaustion occurred during T4 (130.2 ± 340.2 sec) and T5 (157.4 ± 263.1 sec) compared to T2 (455.6 ± 245.0 sec). Plasma sodium concentrations were greater (p < 0.05) at RHY and IP for T2 than all other trials. Aldosterone concentrations at RHY and IP were significantly lower than that at BL and DHY. AVP was significantly elevated at DHY, RHY and IP compared to BL measures. No significant differences were observed between trials in CRP, IL-6, MDA, or in any of the other hormonal or biochemical measures.ConclusionResults demonstrate that AG supplementation provided a significant ergogenic benefit by increasing time to exhaustion during a mild hydration stress. This ergogenic effect was likely mediated by an enhanced fluid and electrolyte uptake.

Effects of Fatigue From Resistance Training on Barbell Back Squat Biomechanics

Abstract Hooper, DR, Szivak, TK, Comstock, BA, Dunn-Lewis, C, Apicella, JM, Kelly, NA, Creighton, BC, Flanagan, SD, Looney, DP, Volek, JS, Maresh, CM, and Kraemer, WJ. Effects of fatigue from resistance training on barbell back squat biomechanics. J Strength Cond Res 28(4): 1127–1134, 2014—Exhaustive resistance training programs that have been previously referred to as extreme conditioning protocols have increased in popularity in military and civilian populations in recent years. However, because of their highly fatiguing nature, proprioception is likely altered during such programs that would significantly affect the safety and efficacy of such programs. Therefore, the purpose of this study was to assess the alterations in movement patterns that result from extreme conditioning protocols and to evaluate if these protocols can be deemed safe and effective. Twelve men (age 24 ± 4.2 years, height 173.1 ± 3.6 cm, weight 76.9 ± 7.8 kg, body fat percentage 9.0 ± 2.2%) and 13 women (age 24.5 ± 3.8 years, height 166.9 ± 8.5 cm, weight 66.1 ± 9.2 kg, body fat percentage 18.6 ± 4.0%) with at least 6 months of resistance training experience involving barbell bench press, barbell deadlift, and barbell back squat performed a highly fatiguing resistance training workout. During the barbell back squat, a 2-dimensional analysis was performed where the knee and hip angles were recorded throughout the 55 repetitions of the workout. At the early stages of the protocol, knee angle was significantly lower in men and in women demonstrating less knee flexion. Also, hip angle was significantly lower early in the program in men and in women, demonstrating a greater forward lean. The technique changes that occur in high repetition sets do not favor optimal strength development and may increase the risk of injury, clearly questioning the safety and efficacy of such resistance training programming. This is likely a display of self-preservation by individuals who are faced with high repetition programs.

Effects of resistance training fatigue on joint biomechanics.

Abstract Hooper, DR, Szivak, TK, DiStefano, LJ, Comstock, BA, Dunn-Lewis, C, Apicella, JM, Kelly, NA, Creighton, BC, Volek, JS, Maresh, CM, and Kraemer, WJ. Effects of resistance training fatigue on joint biomechanics. J Strength Cond Res 27(1): 146–153, 2013—Resistance training has been found to have a multitude of benefits. However, when performed with short rest, resistance training can result in substantial fatigue, which may have a negative impact on exercise technique. The purpose of this study is to examine the effects of fatigue from resistance exercise on joint biomechanics to determine what residual movement effects may exist after the workout. Twelve men with at least 6 months of resistance training experience (age 24 ± 4.2 years, height 173.1 ± 3.6 cm, weight 76.9 ± 7.8 kg) performed 5 body weight squats before (pretest) and after (posttest) a highly fatiguing resistance training workout. Lower extremity biomechanics were assessed using a 3-dimensional motion analysis system during these squats. Peak angle, total displacement, and rate were assessed for knee flexion, trunk flexion, hip flexion, hip rotation, and hip adduction. Results showed a significant decrease in peak angle for knee flexion (Pre: 120.28 ± 11.93°, Post: 104.46 ± 9.85°), hip flexion (Pre: −109.42 ± 12.49°, Post: −95.8 ± 12.30°), and hip adduction (Pre: −23.32 ± 7.04°, Post: −17.30 ± 8.79°). There was a significant reduction in angular displacement for knee flexion (Pre: 115.56 ± 10.55°, Post: 103.35 ± 10.49°), hip flexion (Pre: 97.94 ± 10.69°, Post: 90.51 ± 13.22°), hip adduction (Pre: 17.79 ± 7.36°, Post: 11.89 ± 4.34°), and hip rotation (Pre: 30.72 ± 12.28, Post: 20.48 ± 10.12). There was also a significant reduction in displacement rate for knee flexion (Pre: 2.20 ± 0.20, Post: 1.98 ± 0.20), hip flexion (Pre: 1.92 ± 0.20, Post: 1.76 ± 0.27), hip adduction (Pre: −0.44 ± 0.17, Post: −0.31 ± 0.17), and hip rotation (Pre: 0.59 ± 0.23, Post: 0.38 ± 0.21). This study demonstrated that there are lasting residual effects on movement capabilities after a high-intensity short rest protocol. Thus, strength and conditioning coaches must be careful to monitor movements and exercise techniques after such workouts to prevent injury and optimize subsequent exercise protocols that might be sequenced in order.

A multi-nutrient supplement reduced markers of inflammation and improved physical performance in active individuals of middle to older age: a randomized, double-blind, placebo-controlled study

BackgroundWhile exercise acts to combat inflammation and aging, the ability to exercise may itself be compromised by inflammation and inflammations impact on muscle recovery and joint inflammation. A number of nutritional supplements have been shown to reduce inflammation and improve recovery. The purpose of the current investigation was to examine the effect of a multi-nutrient supplement containing branched chain amino acids, taurine, anti-inflammatory plant extracts, and B vitamins on inflammatory status, endothelial function, physical function, and mood in middle-aged individuals.MethodsThirty-one healthy and active men (N = 16, mean age 56 ± 6.0 yrs) and women (N = 15, mean age = 52 ± 7.5 yrs) participated in this investigation. Subjects completed one 28 day cycle of placebo supplementation and one 28 day cycle of multi-nutrient supplementation (separated by a one week washout period) in a balanced, randomized, double-blind, cross-over design. Subjects completed weekly perceptual logs (PROMIS-57, KOOS) and pre- and post- testing around the supplementation period. Testing consisted of brachial artery flow mediated dilation (FMD), blood measures, and physical performance on vertical jump, handgrip strength, and balance (dispersion from center of pressure). Significance for the investigation was p ≤ 0.05.ResultsIL-6 significantly decreased in both men (from 1.2 ± 0.2 to 0.7 ± 0.4 pg·mL-1) and women (from 1.16 ± 0.04 to 0.7 ± 0.4 pg·mL-1). Perceived energy also improved for both men (placebo: 1.8 ± 0.7; supplement: 3.7 ± 0.8 AUC) and women (placebo: 1.2 ± 0.7; supplement: 2.8 ± 0.8 AUC). Alpha-1-antichymotrypsin (from 108.9 ± 38.6 to 55.5 ± 22.2 ug·mL-1), Creatine Kinase (from 96 ± 34 to 67 ± 23 IU·L-1), general pain, and joint pain decreased in men only, while anxiety and balance (from 0.52 ± 0.13 to 0.45 ± 0.12 cm) improved in women only. Men showed increased performance in vertical jump power (from 2642 ± 244 to 3134 ± 282 W) and grip strength (from 42.1 ± 5.9 to 48.5 ± 4.9 kg).ConclusionsA multi-nutrient supplement is effective in improving inflammatory status in both men and women, markers of pain, joint pain, strength, and power in men only, and both anxiety and balance (a risk factor for hip fracture) in women. Therefore, a multi-nutrient supplement may help middle-aged individuals to prolong physical function and maintain a healthy, active lifestyle.

The Effect of Recovery Time on Subsequent 2K Performance in NCAA Division I Competitive Women Collegiate Rowers

INTRODUCTION: Recovery plays a crucial role in athletic success, yet it is one of the least understood and underresearched areas of sport performance. This study examined the impact of recovery duration on subsequent performance over 2000m on a rowing ergometer using a Concept II-D, (Morrisville, VT) ergometer. METHODS: Ten well-trained, collegiate women rowers (age: 19.4+/2 1.2 yrs, ht: 175.1+/2 5.6, body mass: 63.6+/2 8.5 kg) participated in this investigation. In a randomized, crossover design all subjects completed 2 race trials in each of 2 experimental sessions, passively recovering for either 6 or 24 hours in between trials. Data were collected every 500m; including average power, stroke rate, and 500m split time as well as heart rate and rating of perceived exertion. A one-way analysis of variance (ANOVA) with repeated measures was used to analyze these data and to compare the baseline rowing trials with the subsequent recovery trial of either 6 or 24 hours after the first simulated race of the two day sequence. When appropriate a Fishers LSD post-hoc test was used to determine pair wise differences between the means. Significance in this study was set at P # 0.05. RESULTS: Results showed no statistically significant differences in any of these variables with either the 6 or 24 hour recovery condition or across 500m time points within each race trial. No differences were seen for power changes across the race trials. (see Figure 1). For the RPE values at each 500m segment there were no differences between the recovery time points for any given race segment. However, as might be expected RPE values increased over the course of the race to almost maximal values for the 6-20 scale. The 500m segment RPE values were significantly less than their corresponding values at the end of the 1500m and 2000m race segments. Additionally, the 1000m segment was significantly less than the 2000m race segment, indicating an incremental perceptual