Ozan Deveoglu

IDENTIFICATION BY RP-HPLC-DAD OF NATURAL DYESTUFFS FROM LAKE PIGMENTS PREPARED WITH A MIXTURE OF WELD AND DYER'S OAK DYE PLANTS

The lake pigments by means of the weld (Reseda luteola) and dyers oak (Quercus infectoria) dye plants were prepared by using KAl(SO4)2 · 12H2O (alum), FeSO4 · 7H2O and SnCl2 · 2H2O mordants. A reversed-phase high performance liquid chromatography (RP-HPLC) with diode array detection (DAD) method was utilized for the identification of dyestuffs present in the lake pigments. The dyestuff extractions from the pigments were carried out with a solution mixture of 37% HCl:MeOH:H2O (2:1:1, v/v/v). The performed method is able to analyze and detect natural dyestuffs such as luteolin, apigenin, gallic acid, and ellagic acid present in the lake pigments.

Qualitative HPLC determination of main anthraquinone and lake pigment contents from Dactylopius coccus dye insect

0009-3130/11/4701-0103 2011 Springer Science+Business Media, Inc. 1) Department of Chemistry, Faculty of Science and Letters, Marmara University, 34722, Goztepe, Kadikoy, Istanbul, Turkey, fax: +90 (216) 336 74 68, e-mail: ozan.deveoglu@marmara.edu.tr; 2) Laboratory for Natural Dyes, Faculty of Fine Arts, Marmara University, 34718, Acibadem, Kadikoy, Istanbul, Turkey, e-mail: rkaradag@marmara.edu.tr; 3) Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Marmara University, 34668, Haydarpasa, Istanbul, Turkey, e-mail: tyurdun@marmara.edu.tr. Published in Khimiya Prirodnykh Soedinenii, No. 1, pp. 92–93, January–February, 2011. Original article submitted July 24, 2009. Chemistry of Natural Compounds, Vol. 47, No. 1, March, 2011 [Russian original No. 1, January–February, 2011]

The characterisation by liquid chromatography of lake pigments prepared from European buckthorn (Rhamnus cathartica L.)

Purpose – The purpose of this paper is to prepare, analyse and measure the colour values of the lake pigments obtained from the reaction of Al3+, Fe2+ and Sn2+ metal salts with the natural dyes present in European buckthorn (Rhamnus cathartica L.).Design/methodology/approach – A reversed‐phase high performance liquid chromatography (HPLC) with diode‐array detection method was utilised for the identification of buckthorn lake pigments. The extraction of dyes from the lake pigments was carried out with 37% hydrochloric acid/methanol/water (2:1:1; v/v/v) solution.Findings – According to the results of the HPLC analysis of the lake pigments, it was determined that rhamnetin, kaempferol, and emodin were present in the acid hydrolysed plant extract and in the lake pigment that was precipitated by Sn(II), quercetin‐3‐arabinosid, rhamnetin, and emodin were present in the non‐hydrolysed plant extract, and kaempferol, rhamnetin, isorhamnetin, and emodin were found in the lake pigment that was precipitated by Al(III...

HPLC-DAD analysis of Thymus serpyllum based natural pigments and investigation of their antimicrobial properties

Purpose – In this study, it was aimed to investigate the antibacterial properties of natural pigments prepared from Thymus serpyllum. Design/methodology/approach – Al (III), Fe (II), Sn (II) and Cu (II) complexed natural pigments were obtained by using a precipitation method and the main constituents in the pigments were identified with HPLC-DAD. Also FTIR analysis was performed for further structural characterization. Moreover, the thermal stability and thermal degradation properties of the pigments were analyzed by thermogravimetric analyses (TGA). The antimicrobial activity of the thyme plant-extracted pigments was evaluated by measuring the minimal inhibitory concentration. Findings – Apigenin and luteolin flavones were detected as the main components of the natural dyes. Thermal degradation behaviour of the pigments was determined by means of TGA. All pigments showed high char yields and it was attributed to the high complexation between the metal and the ligand species. The antimicrobial activity of...