Patricia Romagnolli

Segmentos arteriais dos rins de queixadas (Tayassu pecari Link, 1795)

Abstract Was studied 18 pairs of White-lipped peccaries kidneys ( Tayassu pecari ),that has your renal arteries injected whit Vinilit dissolved in P.A. acetonee colored, following submitted to acid corrosion. The vascular moldslike this obtained propose that: a) the renal arteries, always single, todivide firstly in setorial renal branches, being these cranial, dorsal,dorsocranial, dorsocaudal, ventral, ventrocranial, ventrocaudal andcaudal; b) the segmentary renal arteries, originating from seconddivision of the renal arteries, in agreement with your topographicaldisposition, consists of: cranial, mediocranial, middle, mediocaudal,caudal, dorsal and ventral; c) independent of the modality in which tointroduce, the renal segments are separated amongst themselves bypaucivasculars areas. Key-words: White-lipped.Kidney.Renal arteries. Segmentos arteriais dos rins de queixadas ( Tayassu pecari Link, 1795) dorsal, ventrocranial e ventrocaudal(11,11%), e ramos dorsal, ventral e caudal(5,55%); dividiu-se em quatro ramos(38,88%), dorsocranial, dorsocaudal,ventrocranial e ventrocaudal.c) No rim esquerdo, a arteriasetorial renal cranial (77,77%) emitiu asarterias segmentares renais, dorsal (77,77%),media (50%) e ventral (77,77%); a setorialrenal dorsal (88,88%), originou as arteriassegmentares renais, cranial (88,88%),mediocranial (55,55%), media (33,33%),mediocaudal (55,55%) e caudal (88,88%); aarteria setorial renal dorsocranial (11,11%),ramificou-se em arterias segmentares renais,cranial (11,11%), mediocranial (5,55%),mediocaudal (5,55%) e caudal (11,11%); daarteria setorial renal dorsocaudal (11,11%),surgiram as arterias segmentares renais,cranial (11,11%), media (5,55%) e caudal(11,11%); a partir da arteria setorial renalventral (77,77%), tiveram origem as arteriassegmentares renais, cranial (77,77%),mediocranial (61,11%), media (38,88%),mediocaudal (61,11%) e caudal (77,77%); aarteria setorial renal ventrocranial (22,22%)emitiu as arterias segmentares renais, cranial(22,22%), mediocranial (5,55%), media(16,66%), mediocaudal (5,55%) e caudal(22,22%); a arteria setorial renal ventrocaudal(22,22%) ramificou-se em arteriassegmentares renais, cranial (22,22%),mediocranial (5,55%), media (11,11%),mediocaudal (5,55%) e caudal (22,22%).d) No rim esquerdo, a arteriasetorial renal cranial (38,88%), dividiu-se emarterias segmentares renais, dorsal (38,88%),media (27,77%), ventral (38,88%) e caudal(5,55%); a arteria setorial renal dorsal (50%),em arterias segmentares renais, cranial (50%),mediocranial (33,33%), media (38,88%) emediocaudal (33,33%) e caudal (50%); aarteria setorial renal dorsocranial (44, 44%),em arterias segmentares renais, cranial(44,44%), media (16,66%) e caudal (38,88);a arteria setorial renal dorsocaudal (44,44%),em arterias segmentares renais, cranial(44,44%), mediocranial (22,22%), media(22,22%), mediocaudal (22,22%) e caudal(44,44%); a arteria setorial renal ventral(55,55%), em arterias segmentares renais,cranial (55,55%), mediocranial (38,88%),media (22,22%), mediocaudal (38,88) ecaudal (55,55%); a arteria setorial renalventrocranial (38,88%), em arteriassegmentares renais, cranial (38,88%), media(27,77%) e caudal (38,88%); a arteria setorialrenal ventrocaudal (38,88%), em arteriassegmentares renais, cranial (38,88%),mediocranial (16,66%), media (22,22%),mediocaudal (16,66%) e caudal (38,88%); aarteria setorial renal caudal (11,11%), emarterias segmentares renais, dorsal (5,55%),media (5,55%), ventral (5,55%), dorsocranial(5,55%), dorsocaudal (5,55%), ventrocranial(5,55%) e ventrocaudal (5,55%).

Reparo de tendão calcanear comum de coelhos com xenoimplante de artéria carótida preservada em glicerina associado a células mononucleares da medula óssea autólogas

Fifteen adult rabbits were used to evaluate the repair of experimental common calcaneal tendon defects treated with glycerin-preserved canine carotid artery xenografts alone or associated with autologous mononuclear bone marrow cells (AMCs). Rabbits were submitted to daily clinical examination; implanted sites were analyzed under light microscopy within 15, 30 and 60 days of surgery. Pelvic limbs receiving xenografts associated with AMCs had better physical performance as well as higher collagen fiber, fibroblast, lymphocyte and new vessel counts at all postoperative time points considered. Glycerin-preserved canine carotid artery xenografts associated with AMCs constituted an effective method for common calcaneal tendon repair in rabbits.

Decellularized bovine cotyledons may serve as biological scaffolds with preserved vascular arrangement

Technically produced scaffolds are common to establish transplantable tissues for regenerative medicine, but also biological ones that are closer to the natural condition become of interest. Placentas are promising, because they represented available, complete organs with rich extracellular matrix (ECM) and well‐developed vasculature that easily could build anastomoses to a hosts organ. Only placentas from larger animal models such as the bovine meet the dimensions large enough for most organs but are not adequately described yet. We here studied the nature of the ECM in 27 natural and decellularized bovine cotyledons, that is, the fetal part of the placentomes, by means of histology, immunohistochemistry, and electron microscopy. Successful decellularization was done by perfusion with 0.01%, 0.1%, and 0.5% sodium dodecyl sulfate each and subsequent immersion in 1% Triton X‐100, resulting in a removal of cells and DNA, whereas the structure of the allantochorionic surface and villi was preserved. Although some fibres disappeared, also the arrangement of the main ECM proteins was largely similar before and after decellularization: Along the larger vessels, a densely packed network of thick fibres occurred, organized in layers without cells or spaces in between. Collagen IV, fibronectin, and laminin contributed to those areas. In contrast, collagen I and III characterized the meshwork of medium‐sized and thin fibres in the mesenchyme, respectively. In conclusion, decellularized bovine cotyledons indeed had characteristics of a biological scaffold and provide an interesting alternative to develop large‐scale scaffolds with complex vascular architecture for tissue engineering purposes.

Optimization of Canine Placenta Decellularization: An Alternative Source of Biological Scaffolds for Regenerative Medicine

Due to the scarcity of tissues and organs for transplantation, the demand for bioengineered tissues is increasing with the advancement of technologies and new treatments in human and animal regenerative medicine. Thus, decellularized placental extracellular matrix (ECM) has emerged as a new tool for the production of biological scaffolds for subsequent recellularization and implantation for recovery of injured areas or even for replacement of organ and tissue fractions. To be classified as an ideal biological scaffold, the ECM must be acellular and preserve its proteins and physical features to be useful for cellular adhesion. In this context, we developed a process of decellularization of canine placentas with 35 and 40 days of gestation using dodecyl sulfate sodium under immersion and agitation in sterile conditions. Before use of this scaffold in recellularization processes, the decellularization efficiency needs to be confirmed by the absence of cellular content and an irrelevant amount of reminiscent DNA. Both vasculature architecture and ECM proteins, such as collagen types I, III, and IV, laminin, and fibronectin, were preserved with our method. In this way, we established a new biological scaffold model that could be used for recellularization in regenerative medicine of tissues.