Philippe Tacchini
École Polytechnique Fédérale de Lausanne
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Publication
Featured researches published by Philippe Tacchini.
PLOS ONE | 2015
Simona Mrakic-Sposta; Maristella Gussoni; Sarah Moretti; Lorenza Pratali; Guido Giardini; Philippe Tacchini; Cinzia Dellanoce; Alessandro Tonacci; Francesca Mastorci; Andrea Borghini; Michela Montorsi; Alessandra Vezzoli
Purpose Aiming to gain a detailed insight into the physiological mechanisms involved under extreme conditions, a group of experienced ultra-marathon runners, performing the mountain Tor des Géants® ultra-marathon: 330 km trail-run in Valle d’Aosta, 24000 m of positive and negative elevation changes, was monitored. ROS production rate, antioxidant capacity, oxidative damage and inflammation markers were assessed, adopting micro-invasive analytic techniques. Methods Forty-six male athletes (45.04±8.75 yr, 72.6±8.4 kg, 1.76±0.05 m) were tested. Capillary blood and urine were collected before (Pre-), in the middle (Middle-) and immediately after (Post-) Race. Samples were analyzed for: Reactive Oxygen Species (ROS) production by Electron Paramagnetic Resonance; Antioxidant Capacity by Electrochemistry; oxidative damage (8-hydroxy-2-deoxy Guanosine: 8-OH-dG; 8-isoprostane: 8-isoPGF2α) and nitric oxide metabolites by enzymatic assays; inflammatory biomarkers (plasma and urine interleukin-6: IL-6-P and IL-6-U) by enzyme-linked immunosorbent assays (ELISA); Creatinine and Neopterin by HPLC, hematologic (lactate, glucose and hematocrit) and urine parameters by standard analyses. Results Twenty-five athletes finished the race, while twenty-one dropped out of it. A significant increase (Post-Race vs Pre) of the ROS production rate (2.20±0.27 vs 1.65±0.22 μmol.min-1), oxidative damage biomarkers (8-OH-dG: 6.32±2.38 vs 4.16±1.25 ng.mg-1 Creatinine and 8-isoPGF2α: 1404.0±518.30 vs 822.51±448.91 pg.mg-1Creatinine), inflammatory state (IL-6-P: 66.42±36.92 vs 1.29±0.54 pg.mL-1 and IL-6-U: 1.33±0.56 vs 0.71±0.17 pg.mL1) and lactate production (+190%), associated with a decrease of both antioxidant capacity (-7%) and renal function (i.e. Creatinine level +76%) was found. Conclusions The used micro-invasive analytic methods allowed us to perform most of them before, during and immediately after the race directly in the field, by passing the need of storing and transporting samples for further analysis. Considered altogether the investigated variables showed up that exhaustive and prolonged exercise not only promotes the generation of ROS but also induces oxidative stress, transient renal impairment and inflammation.
Oxidative Medicine and Cellular Longevity | 2015
Simona Mrakic-Sposta; Maristella Gussoni; Simone Porcelli; L. Pugliese; Gaspare Pavei; Giuseppe Bellistri; Michela Montorsi; Philippe Tacchini; Alessandra Vezzoli
Acute exercise induces an increase in Reactive Oxygen Species (ROS) production dependent on exercise intensity with highest ROS amount generated by strenuous exercise. However, chronic repetition of exercise, that is, exercise training, may reduce exercise-induced oxidative stress. Aim of this study was to evaluate the effects of 6-weeks high-intensity discontinuous training (HIDT), characterized by repeated variations of intensity and changes of redox potential, on ROS production and antioxidant capacity in sixteen master swimmers. Time course changes of ROS generation were assessed by Electron Paramagnetic Resonance in capillary blood by a microinvasive approach. An incremental arm-ergometer exercise (IE) until exhaustion was carried out at both before (PRE) and after (POST) training (Trg) period. A significant (P < 0.01) increase of ROS production from REST to the END of IE in PRE Trg (2.82 ± 0.66 versus 3.28 ± 0.66 µmol·min−1) was observed. HIDT increased peak oxygen consumption (36.1 ± 4.3 versus 40.6 ± 5.7 mL·kg−1·min−1 PRE and POST Trg, resp.) and the antioxidant capacity (+13%) while it significantly decreased the ROS production both at REST (−20%) and after IE (−25%). The observed link between ROS production, adaptive antioxidant defense mechanisms, and peak oxygen consumption provides new insight into the correlation between ROS response pathways and muscle metabolic function.
Transfusion | 2016
Mélanie Abonnenc; David Crettaz; Philippe Tacchini; Luciana Di Vincenzo; Giona Sonego; Michel Prudent; Jean-Daniel Tissot; Niels Lion
Pathogen inactivation treatments such as INTERCEPT aim to make sure blood and blood‐derived products are free of pathogens before using them for transfusion purposes. At present, there is no established quality control assay that assesses the completeness of the treatment. As INTERCEPT is a photochemical treatment known to generate reactive oxygen species we sought to use the antioxidant power (AOP) of the blood product as a marker of treatment execution. In this perspective, we evaluated an electrochemically based miniaturized system, the EDEL technology, for measuring the AOP in both platelet concentrates (PCs) and plasma.
Vox Sanguinis | 2017
Manon Bardyn; Sunny Isaïe Maye; Andreas Lesch; Julien Delobel; Jean-Daniel Tissot; Fernando Cortés-Salazar; Philippe Tacchini; Niels Lion; Hubert H. Girault; Michel Prudent
Red blood cells (RBCs) suffer from lesions during cold storage, depending in part on their ability to counterbalance oxidative stress by activating their antioxidant defence. The aim of this study was to monitor the antioxidant power (AOP) in erythrocyte concentrates (ECs) during cold storage.
Analytical Chemistry | 2005
Jifeng Liu; Christophe Roussel; Grégoire Lagger; Philippe Tacchini; Hubert H. Girault
Analytical Chemistry | 2006
Jifeng Liu; Bin Su; Grégoire Lagger; Philippe Tacchini; Hubert H. Girault
Journal of Occupational Medicine and Toxicology | 2011
Jean-Jacques Sauvain; Ari Setyan; Pascal Wild; Philippe Tacchini; Grégoire Lagger; Ferdinand Storti; Simon Deslarzes; Michel P. Guillemin; Michel J. Rossi; Michael Riediker
Journal of Electroanalytical Chemistry | 2014
Andreas Lesch; Fernando Cortés-Salazar; Michel Prudent; Julien Delobel; Shokoufeh Rastgar; Niels Lion; Jean-Daniel Tissot; Philippe Tacchini; Hubert H. Girault
Journal of Electroanalytical Chemistry | 2008
Jifeng Liu; Grégoire Lagger; Philippe Tacchini; Hubert H. Girault
Analytical Chemistry | 2015
Andreas Lesch; Fernando Cortés-Salazar; Véronique Amstutz; Philippe Tacchini; Hubert H. Girault