Qing Sun
Sanford-Burnham Institute for Medical Research
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Featured researches published by Qing Sun.
ACS Combinatorial Science | 2015
Aileen Y. Alontaga; Yifei Li; Chih-Hong Chen; Chen-Ting Ma; Siobhan Malany; Danielle Key; Eduard Sergienko; Qing Sun; David A Whipple; Daljit S. Matharu; Baozong Li; Ramir Vega; Yi-Jia Li; Frank J. Schoenen; Brian S. J. Blagg; Thomas Dy Chung; Yuan Chen
Protein-protein interactions are generally challenging to target by small molecules. To address the challenge, we have used a multidisciplinary approach to identify small-molecule disruptors of protein-protein interactions that are mediated by SUMO (small ubiquitin-like modifier) proteins. SUMO modifications have emerged as a target with importance in treating cancer, neurodegenerative disorders, and viral infections. It has been shown that inhibiting SUMO-mediated protein-protein interactions can sensitize cancer cells to chemotherapy and radiation. We have developed highly sensitive assays using time-resolved fluorescence resonance energy transfer (TR-FRET) and fluorescence polarization (FP) that were used for high-throughput screening (HTS) to identify inhibitors for SUMO-dependent protein-protein interactions. Using these assays, we have identified a nonpeptidomimetic small molecule chemotype that binds to SUMO1 but not SUMO2 or 3. NMR chemical shift perturbation studies have shown that the compounds of this chemotype bind to the SUMO1 surface required for protein-protein interaction, despite the high sequence similarity of SUMO1 and SUMO2 and 3 at this surface.
Methods of Molecular Biology | 2013
Eduard Sergienko; Qing Sun; Chen-Ting Ma
Tissue nonspecific alkaline phosphatase (TNAP) is one of four human alkaline phosphatases (AP), a family of exocytic enzymes that catalyze hydrolysis of phospho-monoesters in bone, liver, kidney, and various other tissues. Overexpression of TNAP gives rise to excessive bone and soft tissue mineralization, including blood vessel calcification. Our prior screening campaigns have found several leads against this attractive therapeutic target using in vitro assay with a recombinant enzyme; these compounds were further optimized using medicinal chemistry approaches. To prioritize compounds for their use in animal models, we have designed and developed a biomarker assay for in situ detection of TNAP activity within human and mouse blood samples at physiological pH. This assay is suitable for screening compounds in 1,536-well plates using blood plasma from different mammalian species. The user may choose from two different substrates based on the need for greater assay simplicity or sensitivity.
Archive | 2014
Paul M. Hershberger; Satyamaheshwar Peddibhotla; Eliot Sugarman; Patrick Maloney; Danielle Key; Eigo Suyama; Kevin Nguyen; Stefan Vasile; Martha Kraft; Derek Stonich; Arianna Mangravita-Novo; Michael Vicchiarelli; Palak Gosalia; Monica Milewski; Linda Li; Michael Hedrick; Qing Sun; Eduard Sergienko; Anton Cheltsov; Sumeet Salanawil; Jena Diwan; Layton H. Smith; Russell S. Taichman; Thomas Dy Chung; Anthony B. Pinkerton; Siobhan Malany; Gregory P. Roth
Archive | 2014
Robert Ardecky; Gavin Magnuson; Jiwen Zou; Santhi Ganji; Brock Brown; Tram Ngo; Julia Lee; Fu-Yue Zeng; Qing Sun; Derek Stonich; Sumeet Salaniwal; Tomoyo Sakata; Paul G. Rack; Jennifer K. T. Casabar; Arianna Mangravita-Novo; Layton H. Smith; Eduard Sergienko; Thomas Dy Chung; Anthony B. Pinkerton; Ian Pass; James K. Chen
Archive | 2014
Robert Ardecky; Gavin Magnuson; Jiwen Zou; Santhi Ganji; Brock Brown; Tram Ngo; Julia Lee; Fu-Yue Zeng; Qing Sun; Derek Stonich; Sumeet Salaniwal; Tomoyo Sakata; Paul G. Rack; Jennifer K. T. Casabar; Arianna Mangravita-Novo; Layton H. Smith; Eduard Sergienko; Thomas Dy Chung; Anthony B. Pinkerton; Ian Pass; James K. Chen
Archive | 2014
Robert Ardecky; Gavin Magnuson; Jiwen Zou; Santhi Ganji; Brock Brown; Tram Ngo; Julia Lee; Fu-Yue Zeng; Qing Sun; Derek Stonich; Sumeet Salaniwal; Tomoyo Sakata; Paul G. Rack; Jennifer K. T. Casabar; Arianna Mangravita-Novo; Layton H. Smith; Eduard Sergienko; Thomas Dy Chung; Anthony B. Pinkerton; Ian Pass; James K. Chen
Archive | 2014
Robert Ardecky; Gavin Magnuson; Jiwen Zou; Santhi Ganji; Brock Brown; Tram Ngo; Julia Lee; Fu-Yue Zeng; Qing Sun; Derek Stonich; Sumeet Salaniwal; Tomoyo Sakata; Paul G. Rack; Jennifer K. T. Casabar; Arianna Mangravita-Novo; Layton H. Smith; Eduard Sergienko; Thomas Dy Chung; Anthony B. Pinkerton; Ian Pass; James K. Chen
Archive | 2014
Paul M. Hershberger; Satyamaheshwar Peddibhotla; Eliot Sugarman; Patrick Maloney; Danielle Key; Eigo Suyama; Kevin Nguyen; Stefan Vasile; Martha Kraft; Derek Stonich; Arianna Mangravita-Novo; Michael Vicchiarelli; Palak Gosalia; Monica Milewski; Linda Li; Michael Hedrick; Qing Sun; Eduard Sergienko; Anton Cheltsov; Sumeet Salanawil; Jena Diwan; Layton H. Smith; Russell S. Taichman; Thomas Dy Chung; Anthony B. Pinkerton; Siobhan Malany; Gregory P. Roth
Archive | 2014
Robert Ardecky; Gavin Magnuson; Jiwen Zou; Santhi Ganji; Brock Brown; Tram Ngo; Julia Lee; Fu-Yue Zeng; Qing Sun; Derek Stonich; Sumeet Salaniwal; Tomoyo Sakata; Paul G. Rack; Jennifer K. T. Casabar; Arianna Mangravita-Novo; Layton H. Smith; Eduard Sergienko; Thomas Dy Chung; Anthony B. Pinkerton; Ian Pass; James K. Chen
Archive | 2014
Robert Ardecky; Gavin Magnuson; Jiwen Zou; Santhi Ganji; Brock Brown; Tram Ngo; Julia Lee; Fu-Yue Zeng; Qing Sun; Derek Stonich; Sumeet Salaniwal; Tomoyo Sakata; Paul G. Rack; Jennifer K. T. Casabar; Arianna Mangravita-Novo; Layton H. Smith; Eduard Sergienko; Thomas Dy Chung; Anthony B. Pinkerton; Ian Pass; James K. Chen