Radosav Palić

Synthesis and antimicrobial activity of some new pyrazole derivatives containing a ferrocene unit.

A series of new imines and amines have been synthesized by condensation of 1H-3-ferrocenyl-1-phenylpyrazole-4-carboxaldehyde with the corresponding amines, followed by reduction with sodium borohydride. The synthesized compounds have been screened for their in vitro antimicrobial activity against 11 bacteria and three fungal/yeast strains, using disc diffusion and broth microdilution susceptibility assays. They have shown a wide range of activities, from completely inactive to the highly active compounds.

Even carbon number predominance of plant wax n-alkanes: a correction

Abstract The distributions of n-alkanes of four species of Micromeria have the conventional higher plant pattern of high carbon preference index (CPI) and odd-numbered carbon dominance (maxima at n-C31 or n-C33), rather than the even-numbered predominance previously reported (Palic, R., Ristic, N., Simic, N., Kitic, D., Kapetanovic, R., 1997. The alkanes from some plants of Micromeria genus. Journal of the Serbian Chemical Society 62, 619–622). The stable carbon isotope ratio values (δ13C) of the individual n-alkanes (−38 to −34‰) are typical of C3 plants. Homologous series of odd-numbered predominant iso-alkanes (i-C27 to i-C35) and even-numbered predominant anteiso-alkanes (a-C27 to a-C35) are also present (8 to 18% of the total identified alkanes) and have similar carbon isotopic ratios (−36.8 to−35.1‰).

Chemical composition and antimicrobial activity of the essential oil and CO2 extracts of semi-oriental tobacco, Otlja.

A study of the chemical composition of essential oil and CO2 extracts of the middle and upper leaf positions of the oriental tobacco Prilep was performed using GC and GC–MS. The major constituents were: in the essential oil, neophytadiene and solanone; in CO2 extract I, neophytadiene; and in CO2 extract II, nicotine, nonacosane and solanesol. The antimicrobial activity of the essential oil and CO2 extracts were investigated against the microorganisms: Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The results showed that oil from the middle leaves showed greater activity than the oil of the upper leaves. Both leaf oils mainly showed greater activity than CO2 extracts. Copyright

Chemical composition and antioxidant and antimicrobial activities of essential oil of Allium sphaerocephalon L. subsp. sphaerocephalon (Liliaceae) inflorescences

BACKGROUND Allium sphaerocephalon is a wild-growing plant commonly used as an onion substitute. In this study the essential oil obtained from A. sphaerocephalon subsp. sphaerocephalon inflorescences was analysed by gas chromatography and gas chromatography/mass spectrometry and also evaluated for in vitro antioxidant and antimicrobial activities. The obtained results are reported for the first time and discussed with respect to the sulfur compounds detected in the essential oil. RESULTS Ninty-one compounds were identified, representing 91.6% (w/w) of the total oil. Major constituents of the analysed sample were shyobunol (15.3%), β-caryophyllene (8.1%), α-cadinol (7.8%), 3,5-diethyl-1,2,4-trithiolane (isomer II, 5.9%) and δ-cadinene (5.2%). The diluted oil had an antioxidant capacity of 160 000 ± 111 micromol α-tocopherol acetate equivalents g(-1). Its antimicrobial activity was evaluated against five bacterial and two fungal strains using the broth microdilution assay. Among the micro-organisms tested, the most susceptible strains were Pseudomonas aeruginosa (minimal inhibitory/bactericidal concentration = 0.08/2.5 mg mL(-1)) and Aspergillus niger (minimal inhibitory/fungicidal concentration = 0.31/0.63 mg mL(-1)). CONCLUSION The results showed that the examined species had strong antioxidant and antimicrobial properties and are in accordance with the popular use of plants belonging to the genus Allium in traditional medicine, emphasising the necessity of further detailed study of the active principles in Allium species.

Synthesis and Antimicrobial Activity of New 4-Heteroarylamino Coumarin Derivatives Containing Nitrogen and Sulfur as Heteroatoms

ynthesis, spectral analysis and bioactivity of new coumarin derivatives are described in this paper. Eight new coumarin derivatives were synthesized in moderate to good yields by condensation of 4-chloro-3-nitrocoumarin and the corresponding heteroarylamine. The synthesized compounds were tested for their in vitro antimicrobial activity, in a standard disk diffusion assay, against thirteen strains of bacteria and three fungal strains. They have shown a wide range of activity - from one completely inactive compound to medium active ones.

Composition and Antibacterial Activity of Achillea asplenifolia Essential Oil

Abstract The oil of a wild growing population of Achillea asplenifolia Vent, was studied for yield, composition and antibacterial activity. The major components in the oil were β-caryophyllene (17.6%), germacrene D (15.6%) and chamazulene (13.3%). In an antibacterial diffusion assay, the oil showed activity against all tested Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) and Gram-positive Staphylococcus aureus.

Composition and Antibacterial Activity of Achillea chrysocoma Essential Oil

Abstract Essential oil of Achillea chrysocoma Friv. was investigated for composition and antibacterial activity. Twenty-eight identified compounds constituted 91.5% of the oil. The main compounds of the oil were borneol (10.1%), terpinen-4-ol (9.2%), cis-p-menth-2-en-1-ol (8.2%) and trans-p-menth-2-en-1-ol (7.9%). Antibacterial tests showed pronounced activity of the oil against Gram-negative bacteria Klebsiella pneumoniae and Pseudomonas aeruginosa.

Comparative study of the leaf volatiles of Arctostaphylos uva-ursi (L.) Spreng. and Vaccinium vitis-idaea L. (Ericaceae).

The first GC and GC/MS analyses of the essential oils hydrodistilled from dry leaves of Arctostaphylos uva-ursi and Vaccinium vitis-idaea enabled the identification of 338 components in total (90.4 and 91.7% of the total GC peak areas, respectively). Terpenoids, fatty acids, fatty acid- and carotenoid derived compounds were predominant in the two samples. Both oils were characterized by high relative percentages of α-terpineol and linalool (4.7-17.0%). Compositional data on the volatiles of the presently analyzed and some other Ericaceae taxa (literature data) were mutually compared by means of multivariate statistical analyses (agglomerative hierarchical cluster analysis and principal component analysis). This was done in order to determine, based on the essential oil profiles, possible mutual relationships of the taxa within the family, especially that of species from the genera Arctostaphylos and Vaccinium. Results of the chemical and statistical analyses pointed to a strong relation between the genera Vaccinium and Arctostaphylos.

Composition and Antibacterial Activity of Achillea crithmifolia and Achillea nobilis Essential Oils

Abstract The composition of the essential oils of Achillea crithmifolia and Achillea nobilis was analyzed by GC and GC/MS. The main compounds of the A. crithmifolia essential oil were camphor (27.6%), 1,8-cineole (26.5%) and trans-chrysanthenyl acetate (18.8%), while the most abundant compounds in the A. nobilis oil were α-thujone (25.7%), artemisia ketone (14.8%), borneol (9.9%) and camphor (8.2%). The antibacterial activity of oils was tested for the first time. Both examined oils showed very strong antibacterial activity against all tested microorganisms (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus).

Chemical Composition and Antimicrobial Activity of the Essential Oil of Calamintha nepeta (L.) Savi ssp. glandulosa (Req.) P.W. Ball from Montenegro

Abstract An oil of Calamintha nepeta (L.) Savi ssp. glandulosa (Req.) P. W. Ball obtained by hydrodistillation was analyzed by GC and GC/MS and 36 constituents were identified (98.4%). The main constituents in the oil were pulegone (37.5%), menthone (17,6%), piperitenone (15.0%) and piperitone (10.2%). The antimicrobial activity of the oil was screened against Aspergillus niger, Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, Bacillus subtilis and Pseudomonas aeruginosa. The microorganisms were found to be susceptible to the oil