Reginaldo Inojosa Carneiro Campello

Mortalidade devido a acidentes de bicicletas em Pernambuco, Brasil

O artigo tem por objetivo analisar os casos de morte nos acidentes com bicicleta ocorridos em Pernambuco entre 2001 e 2010. Este estudo quantitativo analisou dados secundarios. A amostra consistiu de todas as Declaracoes de Obitos registradas no Sistema de Informacao de Mortalidade do Banco de Dados do Sistema Unico de Saude que relataram acidentes de bicicleta entre 2001 e 2010. Medidas descritivas foram determinadas para todas as variaveis. As sociodemograficas foram cruzadas com a causa basica de morte em busca de correlacao estatistica. Em Pernambuco, no citado sistema de informacao, foram registradas 517 Declaracoes de Obitos decorrentes de acidentes de bicicleta, sendo nestes sinistros a participacao mais frequente de homens, entre 25-59 anos, pardos, solteiros e de escolaridade ignorada. A idade media foi de 36,82 anos (desvio padrao = 17,026), sendo a idade minima e a maxima, respectivamente, 4 e 86 anos. Os achados apontam para a necessidade da criacao de infraestrutura adequada e de medidas legais efetivas para prevenir acidentes de trafego envolvendo este tipo de veiculo, apoiando-se na evidencia de distribuicao de casos na maioria dos municipios de Pernambuco.

Validation of a reaction volume reduction protocol for analysis of Y chromosome haplotypes targeting DNA databases

The use of Y chromosome haplotypes, important for the detection of sexual crimes in forensics, has gained prominence with the use of databases that incorporate these genetic profiles in their system. Here, we optimized and validated an amplification protocol for Y chromosome profile retrieval in reference samples using lesser materials than those in commercial kits. FTA® cards (Flinders Technology Associates) were used to support the oral cells of male individuals, which were amplified directly using the SwabSolution reagent (Promega). First, we optimized and validated the process to define the volume and cycling conditions. Three reference samples and nineteen 1.2 mm-diameter perforated discs were used per sample. Amplification of one or two discs (samples) with the PowerPlex® Y23 kit (Promega) was performed using 25, 26, and 27 thermal cycles. Twenty percent, 32%, and 100% reagent volumes, one disc, and 26 cycles were used for the control per sample. Thereafter, all samples (N = 270) were amplified using 27 cycles, one disc, and 32% reagents (optimized conditions). Data was analyzed using a study of equilibrium values between fluorophore colors. In the samples analyzed with 20% volume, an imbalance was observed in peak heights, both inside and in-between each dye. In samples amplified with 32% reagents, the values obtained for the intra-color and inter-color standard balance calculations for verification of the quality of the analyzed peaks were similar to those of samples amplified with 100% of the recommended volume. The quality of the profiles obtained with 32% reagents was suitable for insertion into databases.

Mortality due to bicycle accidents in Pernambuco, Brazil

The scope of this paper was to conduct a quantitative analysis of deaths resulting from bicycle accidents in the state of Pernambuco by studying secondary data between 2001 and 2010. The sample consisted of all the Deaths recorded in the Mortality Information System of the Unified Health System Database that reported bicycle accidents between 2001 and 2010. Descriptive measures were determined for all variables. Socio-demographic variables were paired with the basic cause of death in order to find a statistical correlation. In Pernambuco, the aforementioned information system recorded 517 deaths resulting from bicycle accidents, with greater frequency in men between 25 and 59 years of age, Afro-Brazilians, single and of unknown schooling. The mean age was 36.82 years (SD = 17.026), and the minimum and maximum age of 4 and 86 years old, respectively. The findings highlight the need for the creation of adequate infrastructure and effective legal measures to prevent traffic accidents involving this type of vehicle, relying on the evidence of distribution of cases in most Pernambuco municipalities.