Rémi Gervais

Scopolamine injection into the olfactory bulb impairs short-term olfactory memory in rats.

A previous experiment showed that systemic administration of the muscarinic antagonist scopolamine altered delayed matching in an olfactory task in rats. The present experiment tested whether the impairment could result from blockade of the cholinergic transmission in the first relay structure of the olfactory system, the olfactory bulb. Drug was infused directly into both olfactory bulbs before test sessions. Results showed that the intrabulbar infusion reproduced the effect of the systemic administration. With a 4-s delay between target odor and choice test, performances of treated rats remained unchanged; but with a 30-s delay, rats performed randomly. Results from a complementary electrophysiological experiment in anesthetized rats support the idea that scopolamine injected into the olfactory bulb was unlikely to have reached more central structures. Further evidence for the involvement of pure sensory areas in short-term memory is concluded.

Cholinergic modulation of excitability in the rat olfactory bulb: Effect of local application of cholinergic agents on evoked field potentials

The effect of exogenously applied cholinergic agents upon mitral-granule cell complex activity of the olfactory bulb was studied in anesthetized rats. Output neurons were activated by electrical paired-pulse stimulation (40-80 ms time interval) applied either to the olfactory nerve (orthodromic stimulation) or to the lateral olfactory tract (antidromic stimulation). Evoked field potentials were recorded in the granule cell layer. Cholinergic agents were introduced close to the mitral cell body layer through a push-pull cannula. With both orthodromic and antidromic stimulations, acetylcholine in the presence of eserine (an acetylcholinesterase blocker), did not alter the conditioning volley, while it induced a significant increase in the amplitude of the test volley. This effect could be replicated using the cholinergic agonist carbachol. This attenuation of the paired-pulse inhibition is due to a reduction of the dendrodendritic inhibitory action of granule cells upon relay cells. Muscarinic and nicotinic transmission were studied using antidromic and orthodromic stimulations, respectively. The selective effect of acetylcholine on the test volley was totally abolished by the blockade of the muscarinic transmission (by atropine). The blockade of the GABAergic transmission (by picrotoxin), could also prevent the acetylcholine-induced effect. The results lead us to propose that in deep bulbar layers, acetylcholine may activate muscarinic receptors situated on second-order GABAergic interneurons. These interneurons could in turn inhibit granule cells (first-order interneurons). The nicotinic antagonist d-tubocurarine selectively enhanced the duration of the late component and did not appear to modify early components when stimulation was applied to the olfactory nerve. This effect related to both the conditioning and the test volleys and the enhancement in the duration of depolarization of granule cell dendrites suggests that normal activation of nicotinic receptors contributes to a faster repolarization of granule cells. Since nicotinic receptors belong to the outer glomerular layer, this result points to the existence of interneurons belonging to the periglomerular region where they receive nicotinic input and project to deep layers where they modulate granule cell activity. Taken together, our results suggest the presence of a phasic muscarinic and a tonic nicotinic modulation of bulbar interneuronal activity. Since both could finally reduce the inhibitory action of granule cells, the action of cholinergic afferents would facilitate transmission of bulbar output neurons to central structures.

Enduring Effects of Infant Memories: Infant Odor-Shock Conditioning Attenuates Amygdala Activity and Adult Fear Conditioning

BACKGROUNDnEarly life adverse experience alters adult emotional and cognitive development. Here we assess early life learning about adverse experience and its consequences on adult fear conditioning and amygdala activity.nnnMETHODSnNeonatal rats were conditioned daily from 8-12 days-old with paired odor (conditioned stimulus, CS) .5mA shock, unpaired, odor-only, or naive (no infant conditioning). In adulthood, each infant training group was divided into three adult training groups: paired, unpaired or odor-only, using either the same infant CS odor, or a novel adult CS odor without or with the infant CS present as context. Adults were cue tested for freezing (odor in novel environment), with amygdala (14)C 2-DG autoradiography and electrophysiology assessment.nnnRESULTSnInfant paired odor-shock conditioning attenuated adult fear conditioning, but only if the same infant CS odor was used. The (14)C 2-DG activity correlated with infant paired odor-shock conditioning produced attenuated amygdala but heightened olfactory bulb activity. Electrophysiological amygdala assessment further suggests early experience causes changes in amygdala processing as revealed by increased paired-pulse facilitation in adulthood.nnnCONCLUSIONSnThis suggests some enduring effects of early life adversity (shock) are under CS control and dependent upon learning for their impact on later adult fear learning.

A machine learning approach to the analysis of time-frequency maps, and its application to neural dynamics

The statistical analysis of experimentally recorded brain activity patterns may require comparisons between large sets of complex signals in order to find meaningful similarities and differences between signals with large variability. High-level representations such as time-frequency maps convey a wealth of useful information, but they involve a large number of parameters that make statistical investigations of many signals difficult at present. In this paper, we describe a method that performs drastic reduction in the complexity of time-frequency representations through a modelling of the maps by elementary functions. The method is validated on artificial signals and subsequently applied to electrophysiological brain signals (local field potential) recorded from the olfactory bulb of rats while they are trained to recognize odours. From hundreds of experimental recordings, reproducible time-frequency events are detected, and relevant features are extracted, which allow further information processing, such as automatic classification.

What do electrophysiological studies tell us about processing at the olfactory bulb level

Electrophysiological recordings performed in the mammalian olfactory bulb (OB) aimed at deciphering neural rules supporting neural representation of odors. In spite of a fairly large number of available data, no clear picture emerges yet in the mammalian OB. This paper summarizes some important findings and underlines the fact that difference in experimental conditions still represents a major limitation to the emergence of a synthetic view. More specifically, we examine to what extent the absence or the presence of anaesthetic influence OB neuronal responsiveness. In addition, we will see that recordings of either single cell activity or populational activity provide quite different pictures. As a result some experimental approaches provide data underlying sensory properties of OB neurons while others emphasize their capabilities of integrating incoming sensory information with attention, motivation and previous experience.

Extensive immunolesions of basal forebrain cholinergic system impair offspring recognition in sheep.

The involvement of the basal forebrain cholinergic system has been extensively investigated in instrumental learning but little is known of its participation in social memory, especially in the memorization of individual traits of a conspecific. The present study tested in sheep its contribution to both instrumental learning and individual offspring recognition. Six weeks before parturition, ewes received injections of a specific cholinergic immunotoxin (ME20.4 IgG-saporin) into the lateral ventricles (150 microg) and in some cases additional immunotoxin injections into the nucleus basalis (11 microg/side). After 3 weeks of recovery, ewes were trained on a classical instrumental visual discrimination task known to be sensitive to cholinergic deficits. The formation of memory of offspring was assessed through both olfactory and visual/auditory recognition tasks. Olfactory recognition was tested by presenting at suckling successively an alien and the familiar lamb at 2 and 4 h after parturition. Visual/auditory recognition of the lamb was performed using a non-olfactory discrimination test between the familiar and an alien lamb after 12 h of mother-young contact. The lesion extent was assessed by counting choline acetyltransferase-immunopositive neurons in the basal forebrain and measuring the density of acetylcholinesterase fibers in different target areas. Results showed that immunotoxic lesions delayed acquisition of the instrumental visual discrimination. Moreover, olfactory recognition of the lamb was severely impaired while visual/auditory lamb recognition was marginally altered. There was no evidence for sensorimotor or motivational deficits. Importantly, impairment was observed in animals for which loss of basal forebrain cholinergic neurons and their efferent fibers was higher than 75%, while striatal cholinergic neurons and Purkinje cells were unaffected. This study provides evidence that the basal forebrain cholinergic system contributes not only to instrumental but also to social learning. In addition, the cholinergic modulation seems of importance for processing visual and olfactory modalities. However, since only extensive lesions affect performance, this indicates that the basal forebrain cholinergic system possesses substantial reserve capacity to sustain cognitive functions.

ACh-induced long-lasting enhancement in excitability of the olfactory bulb

This study tested whether acetylcholine (ACh) could induce long-term modifications in neuronal excitability in the main olfactory bulb (OB). In anaesthetized rats, perfusion in the region of the mitral cell body layer was done with a push-pull cannula and the neuronal excitability was appraised through evoked field potentials (EFPs) elicited by stimulation of the laternal olfactory tract. Application of ACh (1 mM) for 15 min following a 10 min period of infusion with picrotoxin (GABAA antagonist, 0.4 mM) induced a large enhancement in the antidromic response of output cells which lasted for several hours. This result suggests that the cholinergic projections from the basal forebrain could be involved in processes supporting durable changes in OB response to odours.

Rigorous Training of Dogs Leads to High Accuracy in Human Scent Matching-To-Sample Performance

Human scent identification is based on a matching-to-sample task in which trained dogs are required to compare a scent sample collected from an object found at a crime scene to that of a suspect. Based on dogs’ greater olfactory ability to detect and process odours, this method has been used in forensic investigations to identify the odour of a suspect at a crime scene. The excellent reliability and reproducibility of the method largely depend on rigor in dog training. The present study describes the various steps of training that lead to high sensitivity scores, with dogs matching samples with 90% efficiency when the complexity of the scents presented during the task in the sample is similar to that presented in the in lineups, and specificity reaching a ceiling, with no false alarms in human scent matching-to-sample tasks. This high level of accuracy ensures reliable results in judicial human scent identification tests. Also, our data should convince law enforcement authorities to use these results as official forensic evidence when dogs are trained appropriately.

Olfactory processing controlling food and fluid intake

Publisher Summary This chapter discusses olfactory processing controlling food and fluid intake. Olfactory cues give information on the location and the quality of food, which is critical to the initiation phase of intake. After physical contact is established between the animals mouth and the food, gustatory and other oral cues provide further information on palatability, which ultimately modulates the speed of consumption and the size of the meal. This schematic description of the role of olfaction in the control of intake leads to two important points. Taking a decision regarding whether intake should be initiated requires both fine olfactory discrimination and also a comparison of the incoming information with prior olfactory experiences. The neural bases of these two processes are, therefore, a matter of the coding of olfactory information and the mechanisms involved in olfactory learning and memory.