Renato Abreu Lima

Análise Citotóxica e Mutagênica do Extrato Aquoso de Maytenus Guyanensis Klotzsch ex Reissek (celastraceae) Chichuá (xixuá) Amazônico

O presente estudo objetivou realizar uma analise citotoxica e mutagenica do extrato aquoso de Maytenus guyanensis . Os metodos utilizados foram: indice mitotico, germinacao dos meristemas e analise de micronucleo em A.cepa . Constatou-se que o extrato aquoso da entrecasca M. guianensis nas concentracoes de 3,85 e 38,5 mg/mL nao apresenta acao citotoxica e mutagenica, e ainda atuam como anticitotoxico e antimutagenico. O tratamento de 77mg/mL demonstrou ter acao citotoxica, e anticitotoxica na presenca de paracetamol, essa concentracao nao demonstrou acao mutagenica e antimutagenica. A concentracao de 192mg/mL demonstrou se citotoxica e mutagenica.

The potential of the ethanolic extract of fungicide seed Pachira aquatica on Fusarium sp

O interesse em metabolitos secundarios tem crescido muito nos ultimos anos devido a sua ampla utilizacao como materia prima na preparacao de substâncias com atividade biologica. Portanto, baseado nesse grande interesse, realizou-se o presente trabalho, com o objetivo de avaliar o potencial fungicida do extrato etanolico de sementes de Pachira aquatica (25 mg.mL-1) sobre Fusarium sp. Para obtencao do extrato bruto, o material vegetal foi deixado em maceracao com etanol 95%, durante sete dias, apos isto, o solvente foi evaporado e o extrato etanolico foi obtido. Os ensaios biologicos foram realizados com colonias de F. sp. Os resultados foram submetidos a analise de variância (ANOVA) e comparados pelo teste de Tukey . O crescimento micelial foi avaliado durante quatro e seis dias apos a inoculacao das colonias. Como resultado, observou-se que os diâmetros medios foram de 24 e 27,8 mm, para o genero Fusarium, e 64 e 67,4 mm, para o tratamento controle. A esporulacao foi avaliada no 6o dia apos a inoculacao do fungo e obteve um desenvolvimento de 43 conidios.mL-¹, quando tratados com extrato das sementes e 110 conidios.mL-¹, para o controle. O extrato etanolico das sementes inibiu significativamente o crescimento micelial, esporulacao e foi considerado toxico a Fusarium sp.

Ensinando botânica por meio da confecção de sabonetes de plantas medicinais

Medicinal plants have been used since the dawn of mankind. Vegetable soaps have a greater benefit to human health and the environment, since the chemical composition of the soaps is relatively smaller when compared to commercial soap. In this sense, this work had the objective of making soaps using essences of medicinal plants of the Amazon Region associating with the knowledge in Botany. The work was developed in a full-time school in the municipality of Porto Velho-RO with 120 high school students. The essences of banana, acai, cupuacu and buriti were obtained from free trade shows in Porto Velho-RO. At the school, a pre and a questionnaire was applied in order to get information about the botany in daily life. Then, an oral presentation was made using multimedia resources to perform the work and later, a practical classroom lesson using materials such as: glycerin, essences, natural dye, oven and silicone forms. It was verified that the students understood in a contextualized way the importance of the medicinal plants and their relation with the teaching of Botany, starting to identify that the plants are part of the daily life, thus presenting importance in the area of scientific research

TEACHING EDUCATIONAL PROJECTS-IN BIOLOGY TEACHING IN A PUBLIC SCHOOL IN THE CITY OF PORTO VELHO-RO

This paper argues for teaching biology projects executed in a public school in Porto Velho-RO, stressing the importance of seeking innovative strategies for teaching and learning, assuming that the teacher is rather able to motivate and overcome obstacles. Thus is described the methodology used to project implementation, the objectives to be achieved and the target audience. Explaining each stage thereof. Since the public schools in Brazil face daily challenges, it is concluded that the projects developed in the discipline of biology at the school to provide favorable conditions for student learning, creating an environment of stimulation, where the teacher is a key part of the motivation for these.

Antifungal activity of Hedychium coronarium J. König essential oil against Fusarium oxysporum Schlecht and Thanatephorus cucumeris (A.B. Frank) Donk in vitro

Fusarium oxysporum Schlecht and Thanatephorus cucumeris (A.B. Frank) Donk are fungi which cause some diseases in important plant cultures in Brazil - like bananas and beans. Hedychium coronarium J. Konig is a native plant from Asia that currently occurs in Brazil, where it is used in folk medicine to treat infections in general. The objective of this work was to evaluate the effect of the H. coronarium essential oil from leaves on the in vitro growth of F. oxysporum and T. cucumeris colonies. Discs of 5 mm diameter from isolated cultures of each fungi were placed in the center of 90 mm diameter Petri dishes with potato dextrose agar. In the peripheral area of the dishes four discs of filter paper were placed with 10 µL of essential oil, extracted through distillation system. For a control treatment, discs without essential oil were used. A completely randomized design was used with four replications (of four dishes). Every 24 hours for 8 days the fungi growth was evaluated by measuring the diameter of the colonies. After 192 hours F. oxysporum colonies had 37.4 mm diameter in the treatment with essential oil, while in the control the diameter was 66.4 mm. T. cucumeris colonies reached 11.9 mm diameter with essential oil and covered the dishes in the control. The results showed that the essential oil from leaves of H. coronarium has an inhibitory effect on the in vitro growth of F. oxysporum and T. cucumeris , thus suggesting its potential use in agriculture, mainly with respect to banana and bean cultures. Keywords: phytopathology, medicinal plants, biological control.