Rinaldi Idroes

Eicosapentaenoic Acid (EPA) from Fish Oil and Margarine as Bioactive Compound for Anti-inflammation in Occupational Dermatitis

Occupational dermatitis (OCD) is a skin inflammatory disease caused by allergens and irritant agents in the workplace. The disease is related to hypersensitivity reaction, which is correlated with an immunological mechanism (allergic contact dermatitis) and a nonimmunological mechanism (irritant contact dermatitis). Patients with atopic history (rhinitis allergy, asthma, and atopic dermatitis) have a higher risk of contracting OCD. Atopic individuals suffer from barrier skin damage that increases the risk of allergen and irritant penetration. Inflammatory reaction involves T-helper 1 (Th1), which produces cytokine tumor necrosis factor alpha (TNF-α) and interferon-γ (INF-γ), while T-helper 2 (Th2) produces interleukin (IL-4, IL-6, IL-8, IL-10). Eicosapentaenoic acid (EPA) is an omega-3 substance from polyunsaturated free fatty acids (PUFAs) that has been shown to have an anti-inflammatory effect and the ability to decrease macrophage accumulation. In the inflammatory process, EPA inhibits IL-6, IL-8, and TNF-α, which are mediated by the free fatty acid-binding proteins (FABPs). The aim of this study was to determine the bioactivity compound of EPA for anti-inflammatory agents and its target, based on in silico screening. The bioinformatic tools based on reverse docking used in this study were the PubChem compound database, the protein target prediction database, PharmMapper, SwissTargetPrediction, molecular docking software PyRx 0.8, ligand docking, and binding site analysis using PyMOL How to cite this article: Mohamad Amin, Nanda Earlia, Yuslinda Annisa, Ahya Zhilaliakbar Amin, Cita R.S. Prakoeswa, Rinaldi Idroes, Ihya Fakhrurizal Amin, and Betty Lukiati, (2018), “Eicosapentaenoic Acid (EPA) from Fish Oil and Margarine as Bioactive Compound for Antiinflammation in Occupational Dermatitis” in International Conference of Occupational Health and Safety (ICOHS-2017), KnE Life Sciences, pages 592–598. DOI 10.18502/kls.v4i5.2588 Page 592 Corresponding Author: Mohamad Amin mohamad.amin.fmipa@um.ac.id Received: 15 May 2018 Accepted: 3 June 2018 Published: 19 June 2018 Publishing services provided by Knowledge E Mohamad Amin et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. Selection and Peer-review under the responsibility of the ICOHS 2017 Conference Committee.

A comparative study of emission efficiencies in low-pressure argon plasmas induced by picosecond and nanosecond Nd:YAG lasers

An experimental study is performed on the comparative advantages of nanosecond (ns) and picosecond (ps) lasers in laser-induced breakdown spectroscopy (LIBS) analysis. The experiment focused on the relative efficiencies of the plasma emission induced by the two lasers in low-pressure Ar ambient gas for samples of various hardnesses. It is shown that the emission intensities are consistenly reduced when the ns laser is replaced by the ps laser. This is explained as the consequence of the increased power density delivered by the ps laser, which results in a time mismatch between the passage of the ablated atoms and the formation of the shock wave. The time mismatch in turn leads to less effective thermal excitation by the shock wave plasma and the hence reduced emission intensity. Furthermore, this adverse effect is found to worsen for softer samples due to the slower formation of the shock wave. These results are obtained with the same volumes of craters produced by the two lasers on the same sample, which implies that ns laser irradiation has higher emission efficiency than ps laser irradiation.

Formation and emission characteristics of CN molecules in laser induced low pressure He plasma and its applications to N analysis in coal and fossilization study.

Presented in this paper are the results of an experimental study on the laser induced plasma emission of a number of CN free samples (urea, sucrose) with 40 mJ pulse energy using He and N₂ ambient gases. It is shown that the CN emission has its exclusive sources in the molecules produced as the result of chemical bonding either between the ablated C and N ions in the He plasma or between the ablated C and dissociated N from the N₂ ambient gas. The emission intensities in both cases are found to have the highest values at the low gas pressure of 2 kPa. The emission in He gas is shown to exhibit the typical characteristics related to a shockwave generated excitation mechanism. The experiments using He ambient gas further demonstrate the feasible laser-induced breakdown spectroscopy application to quantitative and sensitive N analysis of coal and promising application for practical in situ carbon dating of fossils.

AKTIVITAS SULFUR DAN SELENIUM NANOPARTIKEL TERHADAP CACING Steinerma feltiae DAN PERBANDINGAN TOKSISITASNYA TERHADAP SEL NEUROBLASTOMA (NEURO 2A CELL LINES)

This study was aimed to investigate the activities of sulphur and selenium nanoparticles against Steinernema feltiae (S. feltiae) and their related toxicities gainst neuroblastoma cells (neuro 2A). Aqueous nanoparticles of sulphur and selenium nanoparticles were characterized with a Zetasizer nano at pH 7 and 25° C. The nematode assay was conducted using S. feltiae. The results showed that aqueous sulphur nanoparticles (SNPs) revealed better activities than aqueous selenium nanoparticles (SeNPs) against S. feltiae with lethal dose 50 (LD50) around 6,99 μg/mL after incubation for 24 hrs. Meanwhile, live and dead assay of cells were conducted by using Neuro 2A cell lines. Live and dead assay showed that aqueous sulphur nanoparticles (SNPs) revealed better activities than aqueous nanoparticles of selenium (SeNPs) against neuroblastoma cells with IC50 was < 1 μg/mL.