Roberto Augusto de Almeida Torres Júnior

Genome-Wide Association for Growth Traits in Canchim Beef Cattle

Studies are being conducted on the applicability of genomic data to improve the accuracy of the selection process in livestock, and genome-wide association studies (GWAS) provide valuable information to enhance the understanding on the genetics of complex traits. The aim of this study was to identify genomic regions and genes that play roles in birth weight (BW), weaning weight adjusted for 210 days of age (WW), and long-yearling weight adjusted for 420 days of age (LYW) in Canchim cattle. GWAS were performed by means of the Generalized Quasi-Likelihood Score (GQLS) method using genotypes from the BovineHD BeadChip and estimated breeding values for BW, WW, and LYW. Data consisted of 285 animals from the Canchim breed and 114 from the MA genetic group (derived from crossings between Charolais sires and ½ Canchim + ½ Zebu dams). After applying a false discovery rate correction at a 10% significance level, a total of 4, 12, and 10 SNPs were significantly associated with BW, WW, and LYW, respectively. These SNPs were surveyed to their corresponding genes or to surrounding genes within a distance of 250 kb. The genes DPP6 (dipeptidyl-peptidase 6) and CLEC3B (C-type lectin domain family 3 member B) were highlighted, considering its functions on the development of the brain and skeletal system, respectively. The GQLS method identified regions on chromosome associated with birth weight, weaning weight, and long-yearling weight in Canchim and MA animals. New candidate regions for body weight traits were detected and some of them have interesting biological functions, of which most have not been previously reported. The observation of QTL reports for body weight traits, covering areas surrounding the genes (SNPs) herein identified provides more evidence for these associations. Future studies targeting these areas could provide further knowledge to uncover the genetic architecture underlying growth traits in Canchim cattle.

Ingestive behaviour, herbage intake and grazing efficiency of beef cattle steers on Tanzania guineagrass subjected to rotational stocking managements

The objective of this experiment was to evaluate the ingestive behaviour, herbage intake and grazing efficiency of beef cattle steers grazing on Panicum maximum Jacq. cv. Tanzania subjected to different rotational stocking intensities. Treatments corresponded to two post-grazing conditions (residues of 25 and 50 cm) associated with a pre-grazing condition of 95% sward canopy light interception during regrowth (LI). The grazing time increased linearly with the duration of the occupation period (475 to 630 minutes/day). On paddocks grazed down to a residue of 25 cm, the bite rate increased linearly along the occupation period, with an average of 42.5 bites/minute. On paddocks grazed down to a residue of 50 cm, the bite rate was stable and equal to 39 bites/minute. There was no difference in herbage intake between grazing intensities. However, grazing at 25 cm residue resulted in greater herbage removal (68.0 vs. 45.6%) and greater grazing efficiency (90.4 vs. 49.8%) than grazing at 50 cm residue. Post-grazing residues of Tanzania guineagrass under rotational stocking management may be set at either 25 or 50 cm, since the herbage intake was not affected within this grazing intensity range. However, herbage removal and grazing efficiency were reduced with the 50 cm post-grazing height and grazing time increased with long occupation periods.

Fatores determinantes do desempenho reprodutivo de vacas Nelore na região dos Cerrados do Brasil Central

The objective of this trial was to evaluate the effects of calving order (CO) and body condition score (BCS), scale 1(thin) to 5 (fat), on reproductive performance of 468 Nellore cows (391 multiparous and 77 primiparous) grazing Brachiaria decumbens Stapf at Brazilian Central West (Cerrados region) during four breeding seasons (from 11/1 to 1/31 of the following year). Calving order quadratically affected pregnancy rate (PR) in this trial; PR of CO1 cows was 69% while that of cows from CO5 to CO8 was 90% followed by a gradual decline until 80% PR in C012 cows. Body condition score at weaning also affected PR. For instance, CO1 cows with BCS of 2.0 and 3.5 had 52.7 and 82.5% of PR, respectively. Cows from CO4 to CO8 and BCS of 3.5 showed 96% of PR. CO1 cows that calved late in the season had 37.7% of PR but independent of CO, cows that calved earlier in the calving season had PR greater than 80%. CO1 cows calved 350.12 days after the start of the mating season while multiparous cows calved earlier (328.32 days). CO1 had the longest calving interval (CI) averaging 392.10 days whereas that from CO5 to CO9 cows averaged 365 days. Calving interval was affected by year, CO, number of days spent to calve in the calving season, and body weight change in the mating season. In addition, cows body weight (BWW) and BC at weaning (BCW) were both affected by CO and year. Body weight of calves at weaning increased from CO1 to CO4/CO5 averaging 159 kg for calves from CO1 cows and 169 kg when all CO levels were included. High production and reproductive performance are obtained from CO3 to CO8 and BCS above 3.0 and 3.5 for multiparous and primiparous Nellore cows, respectively.

Desenvolvimento e desempenho reprodutivo de novilhas Nelore criadas a pasto nos cerrados do Centro-Oeste brasileiro

A model fitted to grazing females Nellore during the rearing periods of the Cerrado Region of Central Brazil was simulated and validated in this study. Body weight gain (BWG) was determined during three years as follows: 1) first dry season after weaning on Brachiaria brizantha (Hochst) Stapf., 2) rainfall season and 3) second dry season both on Panicum maximum Jacq. cv. Tanzânia and averaged 0.020, 0.550, and 0.172 kg, respectively. In the second rainfall season during the rearing period heifers grazed Brachiaria decumbens Stapf. At 24.33 months of age the heifers body weight (BW) averaged 299 kg while in the beginning of the mating season their age and BW averaged, respectively, 25.51 ± 0.99 months (CV = 3.50%) and 309.33 ± 19.19 kg (CV = 6.20%). On average, animals calved 326.71 ± 19.98 days (CV = 6.11) after the beginning of the mating season and 36.26 months of age. The mean pregnancy rate was 88.4%. The pregnant heifers were 25.58 ± 9.97 days older and 9.8 ± 7.04 kg heavier than those non-pregnant. The mean BW and body condition in the middle of the mating season were 360 ± 31.00 and 2.94 ± 0.60, before calving 436 ± 30.86 kg and 4.57 ± 0.40, and at weaning 369 ± 33.89 kg and 3.11 ± 0.64, respectively. The mean weaning rate was of 75.6%. Calves mean BW and age at weaning were of 158 ± 20.1 kg and 202 ± 16.6 days, respectively. Nellore heifers grazing Brachiaria decumbens Stapf. and bred at 24/26 months of age showed high production.

Desempenho e conversão alimentar de novilhos de corte em capim-tanzânia submetido a duas intensidades de pastejo sob lotação rotativa

O experimento foi realizado para avaliar o valor nutritivo da forragem, o desempenho e a conversao alimentar de novilhos em pastos de Panicum maximum Jacq. cv. Tanzânia submetidos a duas intensidades de pastejo rotativo (residuos de 25 e 50 cm), associadas a intervalo de pastejo correspondente ao tempo necessario para que o dossel forrageiro interceptasse 95% da luz incidente durante a rebrotacao. O delineamento experimental utilizado foi o de blocos completos casualizados, com duas repeticoes. Foram avaliados a altura do dossel pre-pastejo, os intervalos de pastejo, o ganho de peso individual, a conversao alimentar, a taxa de lotacao e o ganho de peso por unidade de area. Pastos manejados com o residuo 50 cm apresentaram maior numero de ciclos de pastejo em relacao aqueles manejados com residuo de 25 cm. O maior ganho de peso medio diario foi registrado nos pastos manejados com 50 cm de residuo (801 g/dia) em relacao aqueles manejados com de 25 cm de residuo (664 g/dia). Em contrapartida, a taxa de lotacao foi mais alta nos pastos manejados com o residuo 25 cm, fazendo com que os ganhos de peso por unidade de area fossem 601 e 559 kg/ha para os residuos de 25 e 50 cm, respectivamente. O manejo do pasto visando altura de residuo de 25 cm proporciona maiores ganhos de peso por unidade de area e maior uniformidade de producao e o residuo de 50 cm promove maior ganho individual e maior numero de ciclos de pastejo.

Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale

Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99% for both tests) and specificities (100% for both tests) were confirmed with sera from cattle positive or negative for A. marginale antibodies, respectively, by immunofluorescent antibody test. By the analysis of 583 sera from cattle of three regions of the state of Pernambuco, Brazil, the agreement between both tests was high, with a kappa index of 0.89. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.

Efeitos da everminação de matrizes e de bezerros lactentes em sistema de produção de bovinos de corte na região de Cerrado

The effect of deworming with ivermectin of cows before calving and of suckling calves on fecal egg counts (EPG) and productive performance of two beef cattle herds in Central Brazil was studied. Four groups of pregnant cows received the following treatments: T1- cows and calves not treated, T2- only calves treated, T3- only cows treated, and T4- cows and calves treated. The calves of T2 and T4 were distributed in the following treatments: A- calves treated at 3 to 5 months of age with long action ivermectin, B- treated with ivermectin, and C- control. For the cows, the deworming did not diminish the EPG during lactation and also did not have significant effect on the conception rate, live weight gain and the body weight of their calves at 3 to 5 months of age. The calves of treatment A gained, from the time of treatment to weaning (84 to 108 days), an average of 4.2kg (P=0.0003) and 7.1kg (P<0.0001) more than those of treatment B and C, respectively. The average difference in live weight gain of 2.9kg between the animals of treatment B and C was not significant. The EPG before treatment was not significantly different from the treatments (P=0.8665); but at weaning, the average EPG of the calves from treatment A was lower than for treatment B (P=0.0004) and C (P<0.0001). There was no significant difference in the mean EPG for the calves from treatment B and C.

Produtividade e eficiência de vacas Nelore em pastagem de Brachiaria decumbens Stapf nos Cerrados do Brasil Central

The productivity of Nelore cows on Brachiaria decumbens Stapf pasture was measured during four years, in a soil initially improved with limestone and fertilizer and with free access to a mineral mixture. Pregnancy rate (PR), calving rate (CR), weaning rate (WR), calves weaning body weight (BWC), productivity (P = kg of calf/cow) and efficiency (E= P/100 kg of cow) per cow, productivity per area (AP = calves kg/ha), as well as cow body weight and body condition before the calving season (CLW and CBC), at the middle of the mating season (MLW and MBC) and at weaning (WLW and WBC) were evaluated. Mean PR, CR and WR of 87.5, 81.7 and 77.2%, respectively, were observed. Mean LWC of 170 ± 21.89 kg and mean weaning age of 202 ± 16.48 days were observed. Significant effect of calving order (CO) on LWC was detected. Cows between CO3 and CO9 weaned the heaviest calves. Values of 130 kg and 32.19 kg were observed for P and E, respectively, with AP of 192 kg/ha. The CLW and CBC, MLW and MBC, and WLW and WBC were of 471 + 19.67 kg and 4.45 + 0.30; 405 + 21.48 kg and 3.32 + 0.44; 410 + 9.35 and 3.54 + 0.19, respectively. The correlation between body weight and body condition was of 0.66. Nelore cows maintained on a tropical pastures of Brachiaria decumbens Stapf, in an initial fertilized soil, had high reproduction level and productivity. Cows with medium calving order had higher productivity than primiparous and older cows.

Comparison between indirect enzyme-linked immunosorbent assays for Anaplasma marginale antibodies with recombinant major surface protein 5 and initial body antigens.

Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant major surface protein 5 (rMSP5) and initial body (IB) antigens from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. Both tests showed the same sensitivity (98.2%) and specificities (100% for rMSP5 and 93.8% for IB ELISA) which did not differ statistically. No cross-reactions were detected with Babesia bigemina antibodies, but 5 (rMSP5 ELISA) to 15% (IB ELISA) of cross-reactions were detected with B. bovis antibodies. However, such difference was not statistically significant. Prevalences of seropositive crossbred beef cattle raised extensively in Miranda county, state of Mato Grosso do Sul, Brazil, were 78.1% by rMSP5 ELISA and 79.7% by IB ELISA. In the analysis of sera from dairy calves naturally-infected with A. marginale, the dynamics of antibody production was very similar between both tests, with maternal antibodies reaching the lowest levels at 15-30 days, followed by an increase in the mean optical densities in both ELISAs, suggesting the development of active immunity against A. marginale. Results showed that all calves were seropositive by one-year old, characterizing a situation of enzootic stability. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.

Herdabilidade e correlações quanto a peso, perímetro escrotal e escores visuais à desmama, em bovinos Canchim

The objective of this study was to establish the best-fit model for the genetic evaluation of Canchim cattle and to estimate the genetic parameters for productive characters at weaning. The data used were related to: 12,103 animals, for weight (PD); 5,278, for scrotal circumference (PE), 8,343, for slaughter conformation visual score (CF); 9,111, for sheath and navel scores (UM), and 7,986, for hair coat score (PEL). Statistical models included fixed effects and random additive direct, maternal and maternal permanent environmental effects, in different combinations. The analyses were done by the derivative-free restricted maximum likelihood method. The complete model was the most suited for PD, PE, CF and UM, while the model with only additive direct and additive maternal random effects was the best for PEL. Direct heritability estimates were 0.17, 0.13, 0.20, 0.18, and 0.52 for PD, PE, CF, UM and PEL, respectively, which indicates that it is possible to obtain genetic progress through selection for these traits, mainly for PEL. The additive direct genetic correlations among traits varied from -0.16 to 0.61. The correlations between PD and PE and between PD and CF indicate that selection for PD should result in genetic gain in PE and CF