Rosdiana Natzir

Cloning and expression of MPT83 gene from Mycobacterium tuberculosis in E. coli BL21 as vaccine candidate of tuberculosis: A preliminary study

The appearance of Mycobacterium tuberculosis strains leading to drug resistance has caused new problems in TB treatment in various parts of the world and forces WHO to declare TB as a global emergency. With the increase of TB drug resistance, it is convinced that a more effective vaccine development will stop the epidemic of TB. Some M. tuberculosis antigens, one of which is MPT83, have been examined as TB vaccine candidate. MPT83 antigen, which is very immunogenic in lipoprotein micro bacteria, is identified as surface cell interrelated to antigen with cytometry circulation. Having TB resistance from BCG vaccine, MPT83 is considered TB vaccine candidate that can protect people against TB at adult age. The purpose of this research is to conduct amplification of MPT83 antigen cloning, and expression of its antigen on E. coli bacteria. From the result of the research, it is expected that raw material to produce TB vaccine as well as a high-quality antigen can be obtained. The band of DNA in PCR product is 660 bp, while the one in pGEMT-Easy-Mpt83 recombinant plasmid is 3678 bp. This is expressed in E. coli BL21 strain and produces 48 kDa protein as well as GST-MPT83 fusion protein.

A review: Worldwide medicinal plants for typhoid fever

Typhoid fever, an enteric disease caused by Salmonella typhi, plays an important role of global health matter, contributing to the economic encumbrance in most developed countries. Even more, antibiotic resistance has ensued in Salmonella entericainfection. These health problems have proposed a broad struggle towards more study for new source of antimicrobial products especially from medicinal plants due to more profitable. This study reviews medicinal plants related to the typhoid fever and their mechanisms of antimicrobial action. The investigation for this review were established via the databases PubMed, Google Scholar, online Science Direct, Science and Technology Index. Practically 26 medicinal plants from 23 families used for typhoid fever in worldwide countries were reported. Most of them are originated from African continent, Pakistan and India. Antisalmonella activity was mostly identified by in vitro (MIC and MBC). Generally, the chemical compounds contained in the plants are alkaloid, flavonoid, saponin, tannin, phenols, glycosides, steroid and terpenoid. Their mechanisms of actions are mediation of solute transport inhibition in membranes, affecting the phospholipid membranes of bacterial cell wall, inhibiting nucleic acid synthesis and inducing microbial cell membrane disruption. These results redound to the alternative and complementary medication of typhoid fever.

Expression of Foxp3 mRNA on Preeclampsia with Adaptation Theory

This study aims to determine the expression of mRNA Foxp3 on Preeclampsia and normal pregnant. The research method used is through the quantitative research method using case-control design with the sample size of 20 respondents consisting of 10 cases and 10 controls. The case samples were pregnant women with preeclampsia at the time of the study while control samples were normal pregnant women who met inclusion and exclusion criteria. The data was analyzed using Independent T-Test statistics.

Change of TGF-β1 Gene Expression and TGF-β1 Protein Level in Gingival Crevicular Fluid and Identification of Plaque Bacteria in a Patient with Recurrent Localized Gingival Enlargement before and after Gingivectomy

This case report highlights the change of TGF-β1 gene expressions and TGF-β1 protein level in gingival crevicular fluid (GCF) and identification of plaque bacteria in a patient with recurrent localized gingival enlargement before and after gingivectomy treatment. A 26-year-old woman came to AG Dental Care Clinic, South Sulawesi, Indonesia, in October 2015 with a chief complaint that her gingiva often bled spontaneously and she felt pain on her gingiva and felt less comfortable and no self-confidence with her anterior and posterior gingival condition on the right maxilla region which is slightly larger than normal. She often felt that her gingiva could bleed spontaneously when she was talking or remains silent though. The patient is disturbed by the malodor she felt. At that moment, the patient sought for gingivectomy treatment. Three years afterward, the patient came back with the same complaint. Gingival crevicular fluid has been taken from the gingival sulcus before and after gingivectomy. Clinical and GCF follow-up examination was performed one week and three weeks after gingivectomy, and successful results on biological, functional, and aesthetic parameters were observed.