Sandra Clemencia Pardo Carrasco

Severe combined immunodeficiency with disbalance and functional abnormalities in the T-lymphocyte subsets

Abstract The case of an infant female with severe infections and failure to thrive is reported. Immunological workup demonstrated a severe combined immunodeficiency with normal numbers of circulating T and B lymphocytes. Studies of T-cell subsets showed an absence of T 8 + cells, while the percentage of T 4 + exceeded that of T 3 + cells. Functional studies of the T 4 + subset demonstrated lack of helper activity. When cocultured with normal T cells, B cells from the patients produced in vitro subnormal amounts of IgM, but practically no quantities of IgG and IgA. A lymph node biopsy showed a preserved T-cell-dependent area. Possible pathogenetic mechanisms are discussed.

DNA fragmentation and membrane damage of bocachico Prochilodus magdalenae (Ostariophysi: Prochilodontidae) sperm following cryopreservation with dimethylsulfoxide and glucose

O curimba Prochilodus magdalenae, e uma especie nativa de agua doce da Colombia ameacada de extincao, sendo a mais capturada localmente e uma das mais importantes para a conservacao ex-situ (bancos de germoplasma). O objetivo deste estudo foi avaliar o efeito de tres concentracoes de dimetilsulfoxido (DMSO) (5%, 10%, 15%) e tres de glicose (305, 333, 361 mM) no diluente sobre a fragmentacao do ADN espermatico (F-DNA) (atraves de Halomax®, dispersao da cromatina) e danos em membrana (D-Me) (atraves da coloracao eosina-nigrosina). Depois de avaliar a qualidade espermatica por analise computacional da mobilidade, uma parte do semen dos machos foi diluida separadamente com tres partes do diluente e colocado dentro de canudos de 0.5 ml. O congelamento foi feito em tanque de vapores secos de nitrogenio liquido por 30 minutos e descongelado a 60oC por 8 segundos em banho de agua e avaliada a percentagem de celulas com F-DNA e D-Me. Os resultados demonstraram que a criopreservacao causa grande F-DNA (13.62 ± 1.6% ate 28.91 ± 3.25) e D-Me (24.27 ± 1.1% ate 58.33 ± 2.81%) quando comparada com o semen pre-congelamento (PFS) (6.71 ± 1.54% e 2.34 ± 0.5%, respectivamente para F-DNA e D-Me). Uma interacao significativa foi encontrada entre a concentracao de DMSO e glicose neste experimento. O uso dos diluentes 10% DMSO + 305 mM glicose + 12% gema de ovo de galinha e 10% DMSO + 333 mM glicose + 12% gema de ovo de galinha permitem obter a menor F-DNA e D-Me durante a criopreservacao do semen de curimba. Uma alta correlacao entre F-DNA e D-Me foi encontrada (r = 0.771).

Severe combined immunodeficiency with T lymphocytes retaining functional activity

Two cases of severe combined immunodeficiency (SCID) with normal numbers of T cells are reported. Studies of T-cell subsets showed an absence of TQ1+ lymphocytes and a very low percentage of CD4+ cells in Patient 2. Functional studies of T cells from this patient showed a normal suppressor activity. Patient 1 had normal percentages of T-cell subsets and his lymphocytes showed helper and suppressor activities but to a lesser degree than normal controls. Both cases stressed the heterogeneity of SCID in which T cells could be present and retain some of their functional activities.