Semra Kaya

Sensitivity and specificity of infrared thermography in detection of subclinical mastitis in dairy cows

The objectives of this experiment were to determine interrelationships among mastitis indicators and evaluate the subclinical mastitis detection ability of infrared thermography (IRT) in comparison with the California Mastitis Test (CMT). Somatic cell count (SCC), CMT, and udder skin surface temperature (USST) data were compiled from 62 Brown Swiss dairy cows (days in milk=117+/-51, milk yield=14.7+/-5.2 kg; mean +/- SD). The CORR, REG, and NLIN procedures of Statistical Analysis System (SAS Institute Inc., Cary, NC) were employed to attain interrelationships among mastitis indicators. The diagnostic merit of IRT as an indirect measure of subclinical mastitis was compared with CMT using the receiver operating characteristics curves. The udder skin surface temperature was positively correlated with the CMT score (r=0.86) and SCC (r=0.73). There was an exponential increase in SCC (SCC, x10(3) cells/mL=22.35 x e(1.31 x CMT score); R(2)=0.98) and a linear increase in USST (USST, degrees C=33.45+1.08 x CMT score; R(2)=0.75) as the CMT score increased. As SCC increased, USST increased logarithmically [USST, degrees C=28.72+0.49 x ln(SCC, x10(3) cells/mL); R(2)=0.72]. The USST for healthy quarters (SCC <or=400,000 cells/mL; n=94) was different from that for subclinical mastitic quarters (SCC >400,000 cells/mL; n=135) (mean +/- SE; 33.45+/-0.09 vs. 35.80+/-0.08 degrees C). The sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, positive predictive value, and negative predictive value were 95.6, 93.6, 14.97, 0.05, 95.0, and 93.6, respectively, for IRT and 88.9, 98.9, 83.56, 0.11, 99.2, and 86.1, respectively, for CMT. The area under the receiver operating characteristics curve for IRT and CMT was not different. In conclusion, as a noninvasive and quick tool, IRT can be employed for screening subclinical mastitis via measuring USST, with a high predictive diagnostic ability similar to CMT when microbiological culturing is unavailable. However, the reliability of IRT among cows with different characteristics and those living under various environmental conditions remains to be determined.

Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk.

The aim of this study was to investigate total antioxidant (TAC), and oxidant capacity (TOC) and nitric oxide (NO) levels in milk of cows with subclinical mastitis. Brown Swiss and Holstein breed cows were screened with California Mastitis Test (CMT) to determine mammary glands with subclinical mastitis. Moreover, somatic cell counts (SCC) were determined electronically in all milk samples. Mammary quarters were classified as healthy (n=25) or subclinical mastitis (n=35) based on CMT scores and somatic cell count (SCC: < or =200,000/ml or >200,000/ml) in milk. Nitric oxide, TOC and SCC levels were significantly higher (p<0.001, p<0.005 and p<0.001, respectively) in milk from mammary quarters with subclinical mastitis compared to those from healthy mammary quarters. In conclusion, subclinical mastitis results in higher NO concentrations, TOC and SCC, and NO and TOC were positively correlated with SCC. Moreover, alterations in NO levels and TOC in milk could be used as an alternative diagnostic tool to screen for subclinical mastitis.

Clinical use of deslorelin implants for the long-term contraception in prepubertal bitches: effects on epiphyseal closure, body development, and time to puberty.

Long-acting GnRH agonists have been used both for canine estrus induction and prevention. The objective of the study was to investigate the use of a deslorelin implant as a long-term and reversible contraceptive in prepubertal bitches with special regard to the time of epiphyseal closure. Thirteen healthy, crossbreed, medium-sized prepubertal female dogs were used in this study. An implant containing 9.4 mg (G1, n = 5) and 4.7 mg (G2, n = 4) deslorelin acetate (Suprelorin) or a placebo (sodium chloride 0.9%; G3, n = 4) was inserted subcutaneously in the interscapular region. Estrus was monitored once daily by physical and sexual behavioral changes. Body development, vaginal cytology, and serum progesterone and estradiol 17β concentration were monitored weekly for the first 5 weeks, and then every 3 weeks throughout the treatment period. Radiographic examinations were performed monthly to determine the epiphyseal closure. Half of the deslorelin-treated bitches (G1: n = 2 and G2: n = 2) came into estrus during the 83-week observation period. All animals in the control group showed estrus between the 39th and 64th weeks of observation. Time to puberty averaged 82.7 ± 8.9 and 61.9 ± 9.7 weeks in the deslorelin-treated (G1 and G2) and the control bitches, respectively (P < 0.02). Both deslorelin implants (9.4 and 4.7 mg) can be used efficiently for the long-term prevention of estrus in prepubertal bitches; however, epiphyseal closure is clearly delayed which was without any clinical effect in the present study.

Effect of double GnRH injections on reproductive parameters in Awassi ewes receiving long-term progesterone

ABSTRACT The aim of this study was to investigate the effect of single or double Gonadotropin releasing hormone (GnRH) injections at the time of mating and following on day 9 on pregnancy rates and litter size in ewes. Oestrus was synchronized in 130 adult ewes using intravaginal sponges containing 20 mg of fluorogestone acetate for 14 days. All ewes received 10 IU/kg of pregnant mare serum gonadotropin (PMSG) at the sponge withdrawal. Then animals were divided into three groups. Ewes in group 1 (G1; Control, n = 68) received 1 ml placebo injection. In group 2 (G2; n = 30), ewes in oestrus were treated with 1 ml GnRH (4 μg/ml) at the time of mating. In group 3 (G3; n = 32), ewes were injected 1 ml GnRH not only at the time of mating but also on day 9 post-mating. There was significantly higher in duration of oestrus G1 compared with the G2 and G3. Pregnancy rate was found to be higher in G3 (96.0%) than in G1 (72.7%) and G2 (83.3%). The litter size was significantly higher for G3 (1.26 ± 0.14) in comparison with the G1 (1.18 ± 0.07) and G2 (1.22 ± 0.10). In conclusion, Post-mating GnRH injection enhanced the pregnancy rate and litter size, because of its beneficial effects on embriyo viability by increasing luteal formations.

İneklerde Uterus Enfeksiyonlarında Tedavi Seçenekleri

ÖZET Uterus enfeksiyonları, doğum sırasında oluşan negatif basınç etkisiyle, doğuma yardım girişimleri sonucu veya doğumu takiben oluşan retentio secundinarum, prolapsus uteri ve metabolik hastalıklara bağlı olarak şekillenmektedir. Bunlara ek olara, genital organların muayenesi sırasında veya hatalı intrauterin tedavi-suni tohumlama uygulamalarına bağlı olarak da ortaya çıkabilmektedir. Uterus enfeksiyonları; hipotalamus-hipofiz aksında ve ovaryumda bozukluklara neden olur. Ayrıca, direkt olarak uterus üzerine etkileri de söz konusudur. Böylece enfeksiyonlar; dominant follikül gelişimini baskılar, lutenizian hormon salınımını ve ovülasyonu engeller, korpus luteumun daha küçük ve progesteron üretiminin daha az olmasına neden olur. Fertilitenin düşmesine veya infertiliteye ve süt veriminde azalmaya yol açması gibi ciddi ekonomik kayıplar oluşturmaktadır. Bu nedenle teşhis ve tedavi büyük bir öneme sahiptir. Uterus enfeksiyonlarının formlarına göre değişik tedavi yöntemleri kullanılmaktadır. Tedavide amaç, uterusun kasılmalarını artırarak temizlenmesini ve savunma mekanizmasını uyararak enfeksiyon etkenlerinin kalıcı hâle geçmesini engellemektir. Metrit tedavisinde parenteral antibiyotik tedavisi daha çok tercih edilmesine rağmen endometrit tedavisi çok tartışmalıdır. Antibakteriyel direnç oluşması, antibakteriyel ajanlar üzerine uterus çevresinin etkileri veya uterusun savunma sistemi üzerine tedavi ajanlarının etkilerine bağlı olmak üzere yapılan araştırmalarda çelişkili sonuçlar elde edilmektedir. Bu nedenle özellikle endometritli ineklerde tek bir tedavi yöntemini uygulamak çok zordur. Bu derlemede, ineklerde uterus enfeksiyonlarında kullanılan çeşitli tedavi yöntemleri ve bu yöntemlerin etkililiği, avantaj ve dezavantajları hakkında literatür bilgileri verilmiştir.

CLINICAL, BIOCHEMICAL AND HAEMODYNAMIC EFFECTS OF THE INTRATHECAL KETAMINE FOR OVARIOHYSTERECTOMY IN BITCHES

Abstract The purpose of the study was to evaluate the effects of intrathecal (IT) ketamine HCl anesthesia on clinical values and some haemodynamic and biochemical parameters in bitches. An IT ketamine (10 mg/kg) was administered to 30 bitches with a spinal needle (18-22 G) in the lumbosacral space. The haemodynamic parameters were monitored and some biochemical values were assessed (blood gase, oxygen-haemoglobin, and electrolyte levels). The length and depth of anaesthesia was determined with a pinprick test, touching to the ligamenta lata uteri and incision. Anaesthesia took effect in less than 1 min in all dogs and has lasted an average of 95.9 min. In spite of the fact that the dogs recovered completely from the effects of dissociative anaesthesia, the anaesthesia in the some extremities was observed to be continued for an average of 17 min longer. The use of IT ketamine HCl raised blood pressure and did not have a depressive effect on respiratory and cardiac functions. It was concluded that ketamine HCl could be an appropriate alternative for ovariohysterectomy operations in bitches when the quality of the anaesthesia and the prevention of bradycardia and hypotension are considered.