Serkan Ikiz

Presence and antibiotic susceptibility patterns of contagious mastitis agents (Staphylococcus aureus and Streptococcus agalactiae) isolated from milks of dairy cows with subclinical mastitis

Serkan İKİZ, Beren BAŞARAN, Enver Barış BİNGÖL*, Ömer ÇETİN, Güven KAŞIKÇI, Naciye Yakut ÖZGÜR, Melih UÇMAK, Özge YILMAZ, Mehmet Can GÜNDÜZ, Ahmet SABUNCU Department of Microbiology, Faculty of Veterinary Medicine, İstanbul University, Avcılar, İstanbul, Turkey Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, İstanbul University, Avcılar, İstanbul, Turkey Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, İstanbul University, Avcılar, İstanbul, Turkey

Spectral characterization and antibacterial effect of 2-methyl-6-(5-H-Me-Cl-NO 2-1H-benzimidazol-2-yl)-phenols and some transition metal complexes

Abstract2-Methyl-6-(5-H-methyl-chloro-nitro-1H-benzimidazol-2-yl)-phenols( HLx:x= 1-4)ligands and HL1 complexes with Fe(NO3)3, Cu(NO3)2, AgNO3, Zn(NO3)2 have been synthesized and characterized. The structures of the compounds were confirmed on the basis of elemental analysis, molar conductivity, magnetic moment, FT-IR, 1H-and 13C-NMR. Antibacterial activity of the free ligands, their hydrochloride salts and the complexes were evaluated using the disk diffusion method in dimethyl sulfoxide as well as the minimum inhibitory concentration dilution method, against nine bacteria. While HL1 ligand has not any activity, it’s Ag(I) complex show antibacterial effect toward almost to all the bacteria. Zn(II) complex has antibacterial effect on especially K. pneumoniae, S. epidermidis and S. aureus bacteria.

Recovery of dermatophytes in pet grooming tools from veterinary clinics and pet grooming salons

OBJECTIVES Fomites such as brushes and clippers are known to be potential risk factors for the transmission of dermatophytes between pet animals. The aim of this study was to investigate this risk by examining those grooming implements for the presence of any viable dermatophytes. METHODS For this purpose, samples from 235 pieces of grooming equipment and swab samples from the surface of the 14 grooming tables were taken from 41 veterinary clinics and 3 pet grooming salons. RESULTS Trichophyton tonsurans was isolated from two clipper blades from one veterinary clinic and Microsporum canis were isolated from three clipper blades and one toothbrush from another clinic. CLINICAL SIGNIFICANCE This study shows that current disinfection protocols and grooming procedures of the veterinary clinics and pet grooming salons appear to be adequate.

Synthesis and antinociceptive-antimicrobial activities of some new amide derivatives of 3,5-di/-and 1,3,5-trimethylpyrazoles

Some N-(3,5-di-/1,3,5-trimethylpyrazole-4-yl)-4-substitutedbenzamide derivatives were prepared as possible antiociceptive-antimicrobial agents. New amide derivatives (3–12) were synthesized by reacting 4-amino-3,5-di and 1,3,5-trimethylpyrazoles with 4-substitutedbenzoyl chlorides. Hotplate and tail-immersion tests were used for the determination of the antinociceptive activity. Morphine, was used as a standard test drug. All compounds were administered at a dose of 100 mg/kg ip and some of them had significant antinociceptive activity in both tests. Compound 10 (N-(1,3,5-trimethylpyrazole-4-yl)-4-bromobenzamide), was the most active one in both tests among the compounds. The antinociceptive activity of the compounds 10, 11 (N-(1,3,5-trimethylpyrazole-4-yl)-4-chlorobenzamide), and 12 (N-(1,3,5-trimethylpyrazole-4-yl)-4-fluorobenzamide), started at 30 minutes and continued up to 150 minutes in the hotplate test. Also compounds were tested for their in vitro antimicrobial activity, but exhibited weak antibacterial activity.

Bacterial species isolated from cats with lower urinary tract infection and their susceptibilities to cefovecin

BackgroundThe aim of this study was to determine the bacterial species recovered from 61 cats with lower urinary tract infection (LUTI), and their susceptibility to cefovecin in vitro.ResultsThe clinical signs and final clinical diagnosis for cats with confirmed LUTI were also reported. After physical examination of the cats, urine samples including ≥5-6 leucocytes in microscopic evaluation were cultured using bacteriological techniques. The isolates were identified by conventional microbiological methods and tested for in vitro susceptibility using the Kirby-Bauer disc diffusion method recommended by the Clinical Laboratory Standards Institute. Bacterial growth was observed in 16 of 61 urine samples. Antimicrobial susceptibility tests showed that 13 of 16 (81%) isolates were susceptible to cefovecin. The most frequently isolated bacterium from cats with signs of lower urinary tract infection, was Escherichia coli.ConclusionCefovecin was found to be effective in cats with LUTI. Because cefovecin is a new antimicrobial agent in veterinary medicine, there are only few studies about urine culture of cats with LUTI. It is the first study on in vitro activity of cefovecin against bacterial isolates from cats with lower urinary infections in Istanbul, Turkey.

The Evaluation of Brucella Spp. Isolation Rates in Ruminant Abortion Cases by Using Different Selective Media

Abstract The aim of this study is to evaluate the success of Brucella spp. isolation in ruminant abortion cases by using different selective media. To this end, 58 samples from ruminant abortion cases were utilized. 4 selective media; namely, Farrell Medium (FM), CITA Medium (CM), Modified Thayer Martin (MTM) and Jones & Morgan (JM) were preferred for isolation. In addition to these, one medium with antibiotics was used to extend the range of the results. Suspensions prepared from organ and fetal stomach contents were inoculated to media plates and incubated at 37C° for 5-8 days in 5-10% CO2 condition. Conventional biotyping method was used to identify Brucella isolates within the level of species and biovar. MTM (67.2%) and Farrell (65.5%) outperformed the other media with regards to isolation rate. However, regarding the inhibition ability against contaminant microrganisms, Farrell (86.2%) and CITA (72%) have the highest and second highest percentages respectively. The media’s inhibition ability was examined in the samples in which Brucella spp. isolation occurred to be able to investigate the correlations between isolation and inhibition. Lower isolation percentage was observed in the samples in which the media displayed the lowest inhibition ability against contaminants. In this context, using two different selective media with high inhibition ability against contaminants may be recommended to enhance the isolation rate. Moreover, the components stimulating the growth of Brucella strains might be added to the media to obtain better results.