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Featured researches published by Shila Mortensen.


Veterinary Research | 2009

Rapid and widely disseminated acute phase protein response after experimental bacterial infection of pigs.

Kerstin Skovgaard; Shila Mortensen; Mette Boye; Karin T. Poulsen; Fiona M. Campbell; P. David Eckersall; Peter M. H. Heegaard

The acute phase protein response is a well-described generalized early host response to tissue injury, inflammation and infection, observed as pronounced changes in the concentrations of a number of circulating serum proteins. The biological function of this response and its interplay with other parts of innate host defence reactions remain somewhat elusive. In order to gain new insight into this early host defence response in the context of bacterial infection we studied gene expression changes in peripheral lymphoid tissues as compared to hepatic expression changes, 14–18 h after lung infection in pigs. The lung infection was established with the pig specific respiratory pathogen Actinobacillus pleuropneumoniae. Quantitative real-time PCR based expression analysis were performed on samples from liver, tracheobronchial lymph node, tonsils, spleen and on blood leukocytes, supplemented with measurements of interleukin-6 and selected acute phase proteins in serum. C-reactive protein and serum amyloid A were clearly induced 14–18 h after infection. Extrahepatic expression of acute phase proteins was found to be dramatically altered as a result of the lung infection with an extrahepatic acute phase protein response occurring concomitantly with the hepatic response. This suggests that the acute phase protein response is a more disseminated systemic response than previously thought. The current study provides to our knowledge the first example of porcine extrahepatic expression and regulation of C-reactive protein, haptoglobin, fibrinogen, pig major acute phase protein, and transferrin in peripheral lymphoid tissues.


Innate Immunity | 2010

Hepatic gene expression changes in pigs experimentally infected with the lung pathogen Actinobacillus pleuropneumoniae as analysed with an innate immunity focused microarray.

Kerstin Skovgaard; Shila Mortensen; Mette Boye; Jakob Hedegaard; Peter M. H. Heegaard

Knowledge on gene expression in the liver during respiratory infections is limited although it is well-established that this organ is an important site of synthesis of several systemic innate immune components as response to infections. In the present study, the early transcriptional hepatic response of genes associated with innate immune responses was studied in pigs 14—18 h after intranasal inoculation with Actinobacillus pleuropneumoniae, using innate immune focused microarrays and quantitative real-time PCR (qPCR). The microarray analysis of liver tissue established that 51 genes were differentially expressed. A large group of these genes encoded proteins involved in the acute phase response, including serum amyloid A, C-reactive protein, fibrinogen, haptoglobin and tumor necrosis factor-α the expression of which were all found to be up-regulated and glutathione S-transferase, transthyretin, transferrin and albumin which were down-regulated. Additional genes associated with innate immune responses were investigated using qPCR; genes encoding interleukin-(IL-)1, IL-6, IL-8, lipopolysaccharide binding protein, lactotransferrin, and PigMAP were up-regulated and interferon 1α, α1-acid glycoprotein, mannan-binding lectin A, surfactant protein D, and surfactant protein A1 were down-regulated in the liver of infected animals. Down-regulation of α1-acid glycoprotein during infection has not been described previously in any species. These results confirm that the liver plays an important role in initiating and orchestrating the innate immune response to A. pleuropneumoniae infection.


Acta Veterinaria Scandinavica | 2007

Molecular characterisation of the early response in pigs to experimental infection with Actinobacillus pleuropneumoniae using cDNA microarrays

Jakob Hedegaard; Kerstin Skovgaard; Shila Mortensen; Peter Sørensen; Tim Kåre Jensen; Henrik Hornshøj; Christian Bendixen; Peter M. H. Heegaard

BackgroundThe bacterium Actinobacillus pleuropneumoniae is responsible for porcine pleuropneumonia, a widespread, highly contagious and often fatal respiratory disease of pigs. The general porcine innate immune response after A. pleuropneumoniae infection is still not clarified. The objective of this study was hence to characterise the transcriptional response, measured by using cDNA microarrays, in pigs 24 hours after experimental inoculation with A. pleuropneumoniae.MethodsMicroarray analyses were conducted to reveal genes being differentially expressed in inflamed versus non-inflamed lung tissue sampled from inoculated animals as well as in liver and tracheobronchial lymph node tissue sampled from three inoculated animals versus two non-inoculated animals. The lung samples were studied using a porcine cDNA microarray with 5375 unique PCR products while liver tissue and tracheobronchial lymph node tissue were hybridised to an expanded version of the porcine microarray with 26879 unique PCR products.ResultsA total of 357 genes differed significantly in expression between infected and non-infected lung tissue, 713 genes differed in expression in liver tissue from infected versus non-infected animals and 130 genes differed in expression in tracheobronchial lymph node tissue from infected versus non-infected animals. Among these genes, several have previously been described to be part of a general host response to infections encoding immune response related proteins. In inflamed lung tissue, genes encoding immune activating proteins and other pro-inflammatory mediators of the innate immune response were found to be up-regulated. Genes encoding different acute phase reactants were found to be differentially expressed in the liver.ConclusionThe obtained results are largely in accordance with previous studies of the mammalian immune response. Furthermore, a number of differentially expressed genes have not previously been associated with infection or are presently unidentified. Determination of their specific roles during infection may lead to a better understanding of innate immunity in pigs. Although additional work including more animals is clearly needed to elucidate host response to porcine pleuropneumonia, the results presented in this study demonstrate three subsets of genes consistently expressed at different levels depending upon infection status.


Innate Immunity | 2011

Transcriptional profiling at different sites in lungs of pigs during acute bacterial respiratory infection.

Shila Mortensen; Kerstin Skovgaard; Jakob Hedegaard; Christian Bendixen; Peter M. H. Heegaard

The local transcriptional response was studied in different locations of lungs from pigs experimentally infected with the respiratory pathogen Actinobacillus pleuropneumoniae serotype 5B, using porcine cDNA microarrays. This infection gives rise to well-demarcated infection loci in the lung, characterized by necrotic and haemorrhagic lesions. Lung tissue was sampled from necrotic areas, from visually unaffected areas and from areas bordering on necrotic areas. Expression pattern of these areas from infected pigs was compared to healthy lung tissue from un-infected pigs. Transcription of selected genes important in the innate defence response were further analysed by quantitative real-time reverse-transcriptase PCR. A clear correlation was observed between the number of differentially expressed genes as well as the magnitude of their induction and the sampling location in the infected lung, with the highest number of differentially expressed genes, and the most highly induced genes found in necrotic areas. Interestingly, a group of differentially regulated genes was represented in all three areas, comprising genes encoding cytokines, acute phase proteins, and factors related to regulation of apoptosis and the complement system. Interferon-γ was downregulated in both necrotic and bordering areas. Evidence of neutrophil recruitment was seen by the up-regulation of chemotactic factors for neutrophils. In conclusion, we found subsets of genes expressed at different levels in the three selected areas of the infected lung as compared to the control group. Thus it is demonstrated that an infection with clearly defined infected loci leads to a rapid disseminated intra-organ response in neighbouring seemingly unaffected tissue areas of the infected organ. Within the lung, we found a clear division of induced genes as, in unaffected areas a large part of differently expressed genes were involved in systemic reactions to infections, while differently expressed genes in necrotic areas were mainly concerned with homeostasis regulation.


Scientific Reports | 2016

Late regulation of immune genes and microRNAs in circulating leukocytes in a pig model of influenza A (H1N2) infection

Louise Brogaard; Peter M. H. Heegaard; Lars E. Larsen; Shila Mortensen; Michael Schlegel; Ralf Dürrwald; Kerstin Skovgaard

MicroRNAs (miRNAs) are a class of short regulatory RNA molecules which are implicated in modulating gene expression. Levels of circulating, cell-associated miRNAs in response to influenza A virus (IAV) infection has received limited attention so far. To further understand the temporal dynamics and biological implications of miRNA regulation in circulating leukocytes, we collected blood samples before and after (1, 3, and 14 days) IAV challenge of pigs. Differential expression of miRNAs and innate immune factor mRNA transcripts was analysed using RT-qPCR. A total of 20 miRNAs were regulated after IAV challenge, with the highest number of regulated miRNAs seen on day 14 after infection at which time the infection was cleared. Targets of the regulated miRNAs included genes involved in apoptosis and cell cycle regulation. Significant regulation of both miRNAs and mRNA transcripts at 14 days after challenge points to a protracted effect of IAV infection, potentially affecting the host’s ability to respond to secondary infections. In conclusion, experimental IAV infection of pigs demonstrated the dynamic nature of miRNA and mRNA regulation in circulating leukocytes during and after infection, and revealed the need for further investigation of the potential immunosuppressing effect of miRNA and innate immune signaling after IAV infection.


Veterinary Immunology and Immunopathology | 2007

Validation of putative reference genes for qRT-PCR normalization in tissues and blood from pigs infected with Actinobacillus pleuropneumoniae

Kerstin Skovgaard; Shila Mortensen; Karin T. Poulsen; Øystein Angen; Peter M. H. Heegaard


7th international qPCR & NGS Event Symposium & Industrial Exhibition & Application Workshops | 2016

High-throughput Gene Expression Analysis In Pigs As Model For Respiratory Infections

Kerstin Skovgaard; Louise Brogaard; Kirstine Klitgaard Schou; Lars Erik Larsen; Shila Mortensen; Ralf Dürrwald; M. Schengel; Peter M. H. Heegaard


Conferences and Workshops of COST Action BM1308 | 2014

High-throughput gene expression analysis in pigs as model for respiratory infections

Kerstin Skovgaard; Louise Brogaard; Kirstine Klitgaard Schou; Lars Erik Larsen; Shila Mortensen; Ralf Dürrwald; M. Schengel; Peter M. H. Heegaard


The European Colloquium on Acute Phase Proteins | 2010

Local and disseminated acute phase response during bacterial respiratory infection in pigs

Kerstin Skovgaard; Shila Mortensen; Peter M. H. Heegaard


Veterinary Immunology and Immunopathology | 2009

Microarray analysis of systemic pig innate immune responses after experimental infection with Actinobacillus pleuropneumoniae

Kerstin Skovgaard; Shila Mortensen; Karin T. Poulsen; Gregers Jungersen; Peter M. H. Heegaard

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Peter M. H. Heegaard

Technical University of Denmark

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Kerstin Skovgaard

Technical University of Denmark

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Karin T. Poulsen

Technical University of Denmark

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Louise Brogaard

National Veterinary Institute

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Ralf Dürrwald

Free University of Berlin

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Lars Erik Larsen

Technical University of Denmark

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Mette Boye

Technical University of Denmark

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Gregers Jungersen

Technical University of Denmark

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