Soo Hyeon Kim

Wound healing effect of electrospun silk fibroin nanomatrix in burn-model.

Silk fibroin has recently become an important biomaterial for tissue engineering application. In this study, silk fibroin nanomatrix was fabricated by electrospinning and evaluated as wound dressing material in a burn rat model. The wound size reduction, histological examination, and the quantification of transforming growth factor TGF-β1 and interleukin IL-1α, 6, and 10 were measured to evaluate the healing effects. The silk fibroin nanomatrix treatment exhibited effective performance in decreasing the wound size and epithelialization. Histological finding also revealed that the deposition of collagen in the dermis was organized by covering the wound area in the silk fibroin nanomatrix treated group. The expression level of pro-inflammatory cytokine (IL-1α) was significantly reduced in the injured skin following the silk fibroin nanomatrix treatment compared to the medical gauze (control) at 7 days after burn. Also, the expression level of TGF-β1 in the wound treated with silk fibroin nanomatrix peaked 21-days post-treatment whereas expression level of TGF-β1 was highest at day 7 in the gauze treated group. In conclusion, this data demonstrates that silk fibroin nanomatrix enhances the burn wound healing, suggesting it is a good candidate for burn wound treatment.

Fabrication of duck’s feet collagen–silk hybrid biomaterial for tissue engineering

Collagen constituting the extracellular matrix has been widely used as biocompatible material for human use. In this study, we have selected ducks feet for extracting collagen. A simple method not utilizing harsh chemical had been employed to extract collagen from ducks feet. We fabricated ducks feet collagen/silk hybrid scaffold for the purpose of modifying the degradation rate of ducks feet collagen. This study suggests that extracted collagen from ducks feet is biocompatible and resembles collagen extracted from porcine which is commercially used. Ducks feet collagen is also economically feasible and it could therefore be a good candidate as a tissue engineering material. Further, addition of silk to fabricate a ducks feet collagen/silk hybrid scaffold could enhance the biostability of ducks feet collagen scaffold. Ducks feet collagen/silk scaffold increased the cell viability compared to silk alone. Animal studies also showed that ducks feet collagen/silk scaffold was more biocompatible than silk alone and more biostable than ducks feet or porcine collagen alone. Additionally, the results revealed that ducks feet collagen/silk hybrid scaffold had high porosity, cell infiltration and proliferation. We suggest that ducks feet collagen/silk hybrid scaffold could be used as a dermal substitution for full thickness skin defects.

A comparative mechanical and biocompatibility study of poly(ε-caprolactone), hybrid poly(ε-caprolactone)–silk, and silk nanofibers by colloidal electrospinning technique for tissue engineering:

Poly(ε-caprolactone) is an established polymer used in the fabrication of scaffolds for tissue engineering applications. Poly(ε-caprolactone)’s intrinsic hydrophobicity and toxicity, however, is greater than other natural polymers which limits its applicability. In this study, these problems were addressed by the modification of poly(ε-caprolactone) nanofibers with nanoparticles made from natural polymers, such as silk fibroin. Silk fibroin nanoparticles were prepared by desolvation and blended with poly(ε-caprolactone) to form a colloidal solution capable of forming nanofibers by electrospinning. Fabricated silk fibroin nanoparticles and three different nanofibers were characterized by transmission electron microscopy, variable pressure field emission scanning electron microscope, contact angle, Fourier transform infrared spectroscopy, thermogravimetric analysis, as well as an evaluation of their mechanical properties. The hybrid nanofibers incorporated with silk nanoparticles improved water absorbability compared to pure poly(ε-caprolactone) nanofibers and had much better mechanical properties than the silk fibroin nanofibers. The cytotoxicity and cell attachment tests were carried by culturing NIH 3T3 fibroblasts with the nanofibers. The hybrid nanofibers exhibited better cell viability and cell attachment than the pure poly(ε-caprolactone) nanofibers. Furthermore, the silk fibroin nanoparticles improved the water contact angle and enhanced cell interaction compared to the unmodified poly(ε-caprolactone). Based on these results, the modification of poly(ε-caprolactone) nanofibers with silk nanoparticles is a promising strategy for the improvement of poly(ε-caprolactone)-based nanofibers for future tissue engineering applications.

Fabrication of 3D porous SF/β‐TCP hybrid scaffolds for bone tissue reconstruction

Bio-ceramic is a biomaterial actively studied in the field of bone tissue engineering. But, only certain ceramic materials can resolve the corrosion problem and possess the biological affinity of conventional metal biomaterials. Therefore, the recent development of composites of hybrid composites and polymers has been widely studied. In this study, we aimed to select the best scaffold of silk fibroin and β-TCP hybrid for bone tissue engineering. We fabricated three groups of scaffold such as SF (silk fibroin scaffold), GS (silk fibroin/small granule size of β-TCP scaffold) and GM (silk fibroin/medium granule size of β-TCP scaffold), and we compared the characteristics of each group. During characterization of the scaffold, we used scanning electron microscopy (SEM) and a Fourier transform infrared spectroscopy (FTIR) for structural analysis. We compared the physiological properties of the scaffold regarding the swelling ratio, water uptake and porosity. To evaluate the mechanical properties, we examined the compressive strength of the scaffold. During in vitro testing, we evaluated cell attachment and cell proliferation (CCK-8). Finally, we confirmed in vivo new bone regeneration from the implanted scaffolds using histological staining and micro-CT. From these evaluations, the fabricated scaffold demonstrated high porosity with good inter-pore connectivity, showed good biocompatibility and high compressive strength and modulus. In particular, the present study indicates that the GM scaffold using β-TCP accelerates new bone regeneration of implanted scaffolds. Accordingly, our scaffold is expected to act a useful application in the field of bone tissue engineering.

Novel fabrication method of the peritoneal dialysis filter using silk fibroin with urease fixation system

During the last decade, there has been a great advance in the kidney dialysis system by wearable artificial kidney (WAK) system for end-stage renal disease patients. Uremic solute removal and water regeneration system are the most prerequisite for WAK to work properly. In this study, we designed a filtering membrane system by using immobilized urease silk fibroin filter and evaluated its comparative effectiveness with a PVDF filtering system in peritoneal dialysate regeneration system by urea removal efficacy. We evaluated this membranes characteristic and performances by conducting SEM-EDX analyze, water-binding abilities and porosity test, removal abilities of urea, cytotoxicity assay and enzyme activity assay. Under the condition for optimization of urease, the percentage removal of urea was about 40% and 60% in 50 mg/dL urea solution by urease immobilized PVDF and silk fibroin scaffolds, respectively. The batch experimental result showed that immobilized filter removed more than 50% of urea in 50 mg/dL urea solution. In addition silk fibroin with urease filter removed 90 percent of urea in the peritoneal dialysate after 24 h filtration. We suggest that silk fibroin with urease fixation filter can be used more effectively for peritoneal dialysate regeneration system, which have hydrophilic property and prolonged enzyme activity.

Silk fibroin based hydrogel for regeneration of burn induced wounds

The present study deals with fabrication of hydrogel composed of silk fibroin, calcium alginate and carboxymethyl cellulose. A simple mixing of calcium alginate and carboxymethyl cellulose and silk fibroin was adopted to fabricate new silk fibroin based hydrogel (SFH) type. The adhesive strength, water content and cytotoxicity of fabricated hydrogel was comparatively investigated against standard control as medical gauze (C) and Purilon Gel® (PG). The results from the adhesive strength indicated that presented hydrogels had least adhesive strength but had good cell viability than the C and PG. The cell toxicity results after 1 day of culturing NIH 3T3 fibroblasts indicated that prepared hydrogels comparably promoted cellular growth. The in vivo experiments using Sprague-Dawley were done by creating a second-degree burn on the back of rats to study the effect of healing process. The gross examination investigations at 1, 3, 7, 14 and 21 days indicated a progressive effect induced by the SFH. The histological examinations using Hematoxylin and eosin (H&E) and Masson’s trichrome (MT) staining after 3, 7, 14 and 21 days indicated the complete collagen deposition and dermic formation using the presented SFH. Our results suggest that SFH can be considered as promising new candidate matrices for burn wound regeneration.

Application of a Collagen Patch Derived from Duck Feet in Acute Tympanic Membrane Perforation

We investigated the utility of the duck-feet collagen extraction patching procedure in the traumatic tympanic membrane (TM) perforation via a comparison with spontaneous healing or paper patch. Fifty-six ears of adult male Sprague-Dawley rats, each weighing in the range of 250 to 300 g, were used for the animal studies. Sixteen rats had one-side ear in the control group and the opposite-side ear in the treated groups. The remaining twelve rats had a one-side ear with the duck-feet collagen patch and the opposite-side ear with a paper patch. The proliferating cell nuclear antigen (PCNA) expression cells were calculated among the 200 basal cells, and the expression percentage was identified as the labeling index. The healing of the perforation in the duck-feet collagen patch group was confirmed to be more rapid compared to the healing of the other groups. PCNA staining was observed in the migrating portion of PCNA enhanced cell to collagen scaffold in Post operative day (POD) 7 of collagen patch group. Thus, the adhesive effect of the duck-feet collagen patch to perforated margin was better than that of the paper patch. After completing the healing process, the collagen patch shrank and detached from the tympanic membrane (POD 14). In this study, we confirmed that the use of a duck-feet collagen patch had the advantage of early healing, inducing natural TM contour, and disappearing ability after the patch effect is complete.

Novel transparent collagen film patch derived from duck’s feet for tympanic membrane perforation

Abstract To increase healing rate of tympanic membrane (TM) perforations, patching procedure has been commonly conducted. Biocompatible, biodegradable patching materials which is not limited across cultures is needed. The authors evaluated the effectiveness of novel transparent duck’s feet collagen film (DCF) patch in acute traumatic TM perforation. This procedure was compared with spontaneous healing and paper patching. Cell proliferation features were observed in paper and DCF patches. Forty-eight TMs of 24 rats were used for animal experiment, perforations were made on each TMs, and divided into three groups according to treatment modality. Sixteen were spontaneously healed, 16 were paper patched and 16 were DCF patched. The gross and histological healing results were analyzed. Both paper and DCF patch showed no cytotoxicity, but cell proliferations were more active in DCF than paper in early stage. In animal study, the healing of TM perforations were completed within 14 days in all three groups, but found to be faster in DCF patch group than paper patch or spontaneous healing group. The DCF patches were transparent and size of DCF patches were gradually decreased, so there were no need to remove the DCF patches to check the wound status or after the completion of healing. According to this result, authors concluded that DCF patch is transparent, biocompatible and biodegradable material, and can induce fast healing in acute traumatic TM perforations.